Fig 1: Validation of eight independent prognostic cDEGs. Quantitative real-time PCR analysis was performed to determine the mRNA expression levels of ADH4, AHNAK2, MMAA, and DMBT1 in tumor tissue and adjacent tissues (A), and in recurrent tissues and non-recurrent tissues (B). Western blot analysis was utilized to measure the protein levels of ADH4, AHNAK2, MMAA, and DMBT1 in tumor tissue and adjacent tissues (C), and in recurrent tissues and non-recurrent tissues (D). Data were expressed as mean ± SD of three independent repeats. **p < 0.01, ***p < 0.001, compared with adjacent or non-recurrent tissues. A, adjacent tissues; T, tumor tissues; N, non-recurrent; R, recurrent.