Fig 1: EPDR1 expression in aged human skin. (A) Immunostaining of EPDR1 in human skin dermis. (B) RT-qPCR analysis of gene expression using total RNA extracted from human skin dermis. GAPDH was used as a reference gene. (C) Western blot analysis using proteins extracted from human skin dermis. * p < 0.05. All assays were conducted in experimental triplicate.
Fig 2: EPDR1 expression in aged dermal fibroblasts. (A) Immunostaining of p16ink4a and EPDR1 in young (proliferating) and senescent cells. Merge is a combined fluorescence image of DAPI, p16ink4a, and EPDR1. Bar = 20 µm. (B) RT-qPCR analysis of p16ink4a and EPDR1 gene expression. GAPDH was used as a reference gene. (C) Western blot analysis of EPDR1 expression in young and senescent cells. GAPDH was used as a loading control. (D) ELISA of EPDR1 protein levels in culture medium of senescent cells. (E) Cell viability after recombinant EPDR1 treatment. (F) RT-qPCR analysis of changes in gene expression following the administration of recombinant EPDR1. GAPDH was used as a reference gene. (G) Western blot analysis of protein expression after recombinant EPDR1 treatment. The expression levels of each protein were normalized to the expression level of GAPDH. (H) RT-qPCR analysis of changes in gene expression after EPDR1 knockdown. GAPDH was used as a reference gene. (I) Western blot analysis of protein expression after EPDR1 knockdown. The expression levels of each protein were normalized to the expression level of GAPDH. * p < 0.05. All assays were conducted in experimental triplicate.
Supplier Page from Abcam for Recombinant Human EPDR1 protein