Antarctic Thermolabile UDG M0372S from New England Biolabs

Product Specs
ItemAntarctic Thermolabile UDG
CompanyNew England Biolabs
Price $83.00 Inquire
Catalog NumberM0372S
Size100 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Detection of five viruses commonly implicated with bovine respiratory disease using loop-mediated isothermal amplificationVeterinary QuarterlyDecember 31, 2026Josiah Levi Davidson, Murali Kannan Maruthumuthu, Mohamed Kamel, Suraj Mohan, Ana Pascual-Garrigos, Andres Dextre, Ruth Eunice Centeno-Delphia, Jacquelyn P. Boerman, Deepti Pillai, Jennifer Koziol, et al.adding to 3.5 µL of 100 µM dUTP (Fisher Scientific R0133) and 5.0 uL of Antarctic Thermolabile UDG (NEB M0372) diluted 1:10 in nuclease free water to a single 1.25 mL tube of 2x WarmStart Mastermix (NEB M1800)

(2) ThermiQuant™ AquaStream: A portable instrument for quantitative colorimetric isothermal nucleic acid amplification reactions in paper and tube formatsPLoS OneMay 15, 2026Bibek Raut, Gopal Palla, Jiangshan Wang, Skyler Campbell, Virendra Kumar, Kyungyeon Ra, Mohit S. Verma(100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich,

(3) Mark-Release-Recapture of Packed and Shipped Aedes aegypti with Wolbachia: Implications for Conducting Remote Incompatible Insect Technique ProgramsThe American Journal of Tropical Medicine and HygieneMarch 18, 2025Johanna R. Ohm, Amy Lynd, Austin McGowan, Angel Cupid, Vernessa Bellot, James Q. Le, Evdoxia Kakani, Josh Livni, Jacob E. Crawford, Bradley J. Whiteprimer were each 16 µM in 10X stock), along with 0.5 µL Antarctic Thermolabile UDG (New England BioLabs, M0372) and 0.18 uL deoxyuridine triphosphate (Thermo Fisher, FERR0133) to prevent carryover contamination.

(4) Sample-to-answer detection of miRNA from whole blood using thermally responsive alkane partitionsBiosensors and BioelectronicsMarch 1, 2025Evan H. Benke, David J. Boegner, Alejandra M. Bogusch, Ian M. WhiteSplintR Ligase (NEB#M0375), Bst 2.0 WarmStart DNA Plolymerase (NEB#M0538), Antarctic Thermolabile UDG (NEB#M0372), LAMP Fluorescent Dye (NEB#B1700), dNTP Solution Set (NEB#N0446), and dUTP solution (NEB#N0459). All

(5) Combined forces of hydrostatic pressure and actin polymerization drive endothelial tip cell migration and sprouting angiogenesisElifeFebruary 20, 2025Igor Kondrychyn, Liqun He, Haymar Wint, Christer Betsholtz, Li-Kun PhngM3003L), 1 µl cDNA (twofold dilution), 0.2 µl antarctic thermolabile UDG (New England BioLabs, Cat# M0372L), and 250 nM each primer to a final volume of 10 µl. Reactions were run in 384-well plates (Applied

(6) Combined forces of hydrostatic pressure and actin polymerization drive endothelial tip cell migration and sprouting angiogenesisElifeFebruary 20, 2025Igor Kondrychyn, Liqun He, Haymar Wint, Christer Betsholtz, Li-Kun PhngM3003L), 1 µl cDNA (twofold dilution), 0.2 µl antarctic thermolabile UDG (New England BioLabs, Cat# M0372L), and 250 nM each primer to a final volume of 10 µl. Reactions were run in 384-well plates (Applied

(7) A drop dispenser for simplifying on-farm detection of foodborne pathogensPLOS ONEDecember 31, 2024Mohsen Ranjbaran, Simerdeep Kaur, Jiangshan Wang, Bibek Raut, Mohit S. Verma(100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst2.0 DNA Polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red solution (25

(8) Digital droplet RT‐LAMP increases speed of SARS‐CoV‐2 viral RNA detectionSmart MedJune 5, 2024Yuan Yuan, Perry Ellis, Ye Tao, Dimitri A. Bikos, Emma K. Loveday, Mallory M. Thomas, James N. Wilking, Connie B. Chang, Fangfu Ye, David A. WeitzMix (NEB M1700S), 0.175 μL 100 mM dUTP (NEB N0459S), 0.5 μL 1000 U/mL Antarctic Thermolabile UDG (NEB M0372L), 2.5 μL 2 M Guanidine HCl (Promega H5381), 2.325 μL of 5 mM TCEP (EMD Millipore 51805), 0.5 μL of 25

(9) Digital droplet RT‐LAMP increases speed of SARS‐CoV‐2 viral RNA detectionSmart MedicineJune 5, 2024Yuan Yuan, Perry Ellis, Ye Tao, Dimitri A. Bikos, Emma K. Loveday, Mallory M. Thomas, James N. Wilking, Connie B. Chang, Fangfu Ye, David A. WeitzMix (NEB M1700S), 0.175 μL 100 mM dUTP (NEB N0459S), 0.5 μL 1000 U/mL Antarctic Thermolabile UDG (NEB M0372L), 2.5 μL 2 M Guanidine HCl (Promega H5381), 2.325 μL of 5 mM TCEP (EMD Millipore 51805), 0.5 μL of 25

(10) Vivid COVID-19 LAMP is an ultrasensitive, quadruplexed test using LNA-modified primers and a zinc ion and 5-Br-PAPS colorimetric detection systemCommunications BiologyMarch 2, 2023Adrián Szobi, Katarína Buranovská, Nina Vojtaššáková, Daniel Lovíšek, Halil Önder Özbaşak, Sandra Szeibeczederová, Liudmyla Kapustian, Zuzana Hudáčová, Viera Kováčová, Diana Drobná, et al.WarmStart® DNA polymerase (M0538), WarmStart® RTx Reverse Transcriptase (M0380), Antarctic Thermolabile UDG (M0372), WarmStart® Master Mix (M1800) and Extreme Thermostable Single-Stranded DNA Binding Protein (ET SSB;

(11) DddA homolog search and engineering expand sequence compatibility of mitochondrial base editingNature CommunicationsFebruary 16, 2023Li Mi, Ming Shi, Yu-Xuan Li, Gang Xie, Xichen Rao, Damu Wu, Aimin Cheng, Mengxiao Niu, Fengli Xu, Ying Yu, et al.incubated for 1 h at 37 °C, followed by the addition of 5 μL of UDG reaction solution (New England Biolabs, M0372S; 0.02 U/μL UDG in 1×UDG buffer) and an additional 30 min incubation at 37 °C. Cleavage of abasic sites

(12) Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnosticsScientific ReportsDecember 11, 2022Bruna Winkert Raddatz, Edson Yu Sin Kim, Louise Matiê Imamura, Gisleine Jarenko Steil, Erika Bergamo Santiago, Santiago Pedro Timm Soares, Victor Henrique Alves Ribeiro, Bernardo Montesanti Machado de Almeida, Sergio Renato Rogal, Marcus Vinícius Mazega Figueredomethods Reagents and equipment Bst 2.0 WarmStart® DNA Polymerase (M0538), Antarctic Thermolabile UDG (M0372), WarmStart® RTx Reverse Transcriptase (M0380), as well as dNTPs (N0446S and N0459S) were acquired from

(13) Single-copy detection of somatic variants from solid and liquid biopsyScientific ReportsDecember 1, 2021Ana-Luisa Silva, Paulina Klaudyna Powalowska, Magdalena Stolarek, Eleanor Ruth Gray, Rebecca Natalie Palmer, Bram Herman, Cameron Alexander Frayling, Barnaby William BalmforthU Hot Start Polymerase (F555, Thermo Fisher Scientific), 0.5 µL Antarctic Thermolabile UDG (1 U/µL, M0372, New England Biolabs) and 2–20 ng DNA template in a 50 µL reaction. PCR amplification of EGFR exons

(14) Field-deployable, rapid diagnostic testing of saliva for SARS-CoV-2Scientific ReportsDecember 1, 2021Shan Wei et al.(Figure S1A) with the addition of 0.14 µM dUTP and 0.0002 unit/µL Antarctic Thermolabile UDG (NEB, M0372S). Primer set V5 was designed to target a central portion of the SARS-CoV-2 genome and was able to detect

(15) Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extractionScientific ReportsDecember 1, 2021Shan Wei, Esther Kohl, Alexandre Djandji, Stephanie Morgan, Susan Whittier, Mahesh Mansukhani, Eldad Hod, Mary D’Alton, Yousin Suh, Zev Williamsprepared by adding 3.5 µL 100 mM dUTP (Thermo Scientific, R0133), 0.5 µL Antarctic Thermolabile UDG (NEB, M0372S), and 0.25 µL 5 mM SYTO 9 (Invitrogen, S34854) to 1250 µL WarmStart Colorimetric LAMP 2X Master Mix

(16) Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2MethodsXNovember 20, 2021Jiangshan Wang, Andres Dextre, Ana Pascual-Garrigos, Josiah Levi Davidson, Murali K. Maruthamuthu, Darby McChesney, Jordan Seville, Mohit S. VermaUSA), Deoxyuridine Triphosphate (dUTP) (FERR0133, Thermo Scientific, USA), Antarctic Thermolabile UDG (M0372S, New England Biolabs, USA), Polysorbate 20 (278632500, Thermo Scientific Chemicals, USA), Betaine (B0300-5VL,

(17) Synthesis of Libraries and Multi-Site Mutagenesis using a PCR derived, dU-containing TemplateSynthetic BiologyJanuary 5, 2021Gretchen Meinke, Nahide Dalda, Benjamin S Brigham, Andrew Bohmwithout prior treatment with Antarctic Thermolabile Uracil DNA glycosylase (UDG) (New England Biolabs, M0372S). For this test, a series 10-fold dilutions of the ssDNA is used as the template: none, 1:10, 1:100,

(18) Integrated digital error suppression for improved detection of circulating tumor DNANature BiotechnologyMay 1, 2016Aaron M. Newman et al.healthy adult donor using the following products: (i) uracil DNA-glycosylase (UDG; NEB catalog number M0372S), which leaves an abasic site in place of uracil (a cytosine oxidation product), thereby preventing

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Antarctic Thermolabile UDG

Antarctic Thermolabile UDG from New England Biolabs

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Antarctic Thermolabile UDG from New England Biolabs

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