Fig 1: Coactivation of the Hippo and mTORC1 pathways in cancer.a, b Immunohistochemical staining using antibodies against YAP1 (a) and phospho-p70S6K1 (b) in human breast cancer tissues. Upper panel: representative images (scale bars, 100 µm); lower panel: statistical analysis of immunohistochemical staining (***P < 0.001). The results are the mean ± s.e.m. of n = 3 independent experiments. P values were determined by one-way ANOVA. c Pearson’s correlation analysis comparing staining density between YAP1 and phospho-p70S6K1, n = 20. d, e Immunohistochemical staining using antibodies against YAP1 (d) and phospho-p70S6K1 (e) in human cholangiocarcinoma tissues. Upper panel: representative images (scale bars, 400 µm); lower panel: statistical analysis of immunohistochemical staining (***P < 0.001). The results are the mean ± s.e.m. of n = 3 independent experiments. P values were determined by one-way ANOVA. f Pearson’s correlation analysis comparing staining density between YAP1 and phospho-p70S6K1, n = 20.