Annexin V-FITC 130-097-928 from Miltenyi Biotec

(1) NK-A 17E-233I: a novel competitive inhibitor of human dihydroorotate dehydrogenase (DHODH) for cancer therapyJ Exp Clin Cancer ResOctober 17, 2025Mohammed Moustapha Anwar, Salvador Meseguer, Néstor García-Rodríguez, Ewa Krupinska, Céleste Sele, Aida Rodríguez-Jiménez, Suraj Verma, Samna Sagadevan, Javier Ramon, Ramon Martí, et al.distilled water, 5.5 mg CaCl2, 164 mg NaCl, and 2 mL of 100 mM HEPES. A 1:1 mixture of Annexin V (#130–097-928; Miltenyi Biotec) and propidium iodide (PI, 1 mg/mL) was added to all tubes and incubated in the dark

(2) Migration of human T cells can be differentially directed by electric fields depending on the extracellular microenvironmentiScienceMay 17, 2024Karen Ende, Fabião Santos, Judith Guasch, Ralf Kemkemerproteins" ,, Bovine Collagen I (5 mg/mL),Viscofan,Cat# 500060635 Annexin V - FITC,Miltenyi Biotec,Cat# 130-097-928 Dynabeads human T-Activator CD3/CD28,Gibco,Cat# 11131D Human FN (fibronectin) (1 mg/mL),Gibco,Cat#

(3) Repurposing the Bis-Biguanide Alexidine in Combination with Tyrosine Kinase Inhibitors to Eliminate Leukemic Stem/Progenitor Cells in Chronic Myeloid LeukemiaCancersFebruary 3, 2023Muselli F, Mourgues L, Rochet N, Nebout M, Guerci A, Verhoeyen E, Krug A, Legros L, Peyron JF, Mary D.cells were washed with PBS/2% FCS, and then stained in FITC–annexin-V-containing buffer (Miltenyi, 130-097-928, Bergisch Gladbach, Germany) and DAPI (Sigma, D9542-5MG, St. Louis, MO, USA) according to the manufacturer’s

(4) Reprogramming monocyte-derived macrophages through caspase inhibitionOncoImmunologyMarch 7, 2022Paul Chaintreuil et al.specific buffer (10 mM HEPES, 150 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2) with AnnexinV-FITC (Miltenyi, 130–097-928) and DAPI (Sigma-Aldrich, D9542). Fluorescence was measured with a MACSQuant® Analyzer (Miltenyi, Paris,

(5) Armored BCMA CAR T Cells Eliminate Multiple Myeloma and Are Resistant to the Suppressive Effects of TGF-βFrontiers in ImmunologyFebruary 9, 2022Leah M. Alabanza, Ying Xiong, Bang Vu, Brian Webster, Darong Wu, Peirong Hu, Zhongyu Zhu, Boro Dropulic, Pradyot Dash, Dina SchneiderT cells were stained with antibody to CD107a, or annexin V-FITC binding reagent (Miltenyi Biotec, Cat# 130-097-928). All antibodies were from Miltenyi Biotec (Auburn CA), unless otherwise noted. After staining, cells

(6) Reprogramming monocyte-derived macrophages through caspase inhibitionOncoimmunologyDecember 30, 2021Paul Chaintreuil, Lucie Laplane, Florian Esnault, Victoria Ghesquier, Coline Savy, Nathan Furstoss, Marie-Laure Arcangeli, Thomas Cluzeau, Guillaume Robert, Nathalie Droin, et al.specific buffer (10 mM HEPES, 150 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2) with AnnexinV-FITC (Miltenyi, 130–097-928) and DAPI (Sigma-Aldrich, D9542). Fluorescence was measured with a MACSQuant® Analyzer (Miltenyi, Paris,

(7) Ascorbic Acid Inhibits Liver Cancer Growth and Metastasis in vitro and in vivo, Independent of Stemness Gene RegulationFrontiers in PharmacologyAugust 24, 2021Jingjing Wan, Juan Zhou, Lu Fu, Yubin Li, Huawu Zeng, Xike Xu, Chao Lv, Huizi Jin106 single cells were resuspended and mixed with 10 μl Annexin V-fluorescein isothiocyanate (FITC, 130-097-928, Miltenyi Biotec), incubated in darkness for 15 min, washed with 1 ml 1× Annexin V Binding Buffer and

(8) Combination of PKCδ Inhibition with Conventional TKI Treatment to Target CML ModelsCancersApril 2, 2021Muselli F, Mourgues L, Morcos R, Rochet N, Nebout M, Guerci-Bresler A, Faller DV, William RM, Mhaidly R, Verhoeyen E, et al.saline (PBS) with 2% FCS, and then stained in a buffer containing FITC Annexin V (Miltenyi Biotec, 130-097-928) and DAPI (Sigma, D9542-5MG) for 15 min at room temperature (◦ C) according to the manufacturer’s protocol.

(9) Use of p53‐Silenced Endothelial Progenitor Cells to Treat Ischemia in Diabetic Peripheral Vascular DiseaseJournal of the American Heart AssociationApril 5, 2017Nabanita Kundu, Cleyton C. Domingues, Cyril Chou, Neeki Ahmadi, Sara Houston, D. Joseph Jerry, Sabyasachi Senexposure were stained with propidium iodide (Miltenyi Biotec, 130-093-233) and annexin V (Miltenyi Biotec, 130-097-928) along with suggested isotype control using Miltenyi Biotec standard protocols. Both human EPCs (CD34+)

(10) Platelet dysfunction in hypercholesterolemia mice, two Alzheimer's disease mouse models and in human patients with Alzheimer's diseaseBiogerontologyAugust 1, 2015Plagg B, Marksteiner J, Kniewallner KM, Humpel C.study apoptosis, 2 µl of platelets were incubated with 5 µl Annexin V-FITC (Milteny Biotec, Order no.: 130-097-928) in 100 µl Annexin V Binding Buffer (Miltenyi Biotec, 20x Stock Solution) at RT for 30 min in dark.

(11) Annexin V binds to positively selected B cellsJournal of immunology (Baltimore, Md. : 1950)January 1, 2001Dillon SR, Constantinescu A, Schlissel MS.

(12) A receptor for phosphatidylserine-specific clearance of apoptotic cellsNatureMay 4, 2000Fadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RA, Henson PM.

(13) Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assayCytometryAugust 1, 1999Demo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, et al.

(14) Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin VBloodFebruary 1, 1996Kuypers FA, Lewis RA, Hua M, Schott MA, Discher D, Ernst JD, Lubin BH.

(15) Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and AblThe Journal of experimental medicineNovember 1, 1995Martin SJ, Reutelingsperger CP, McGahon AJ, Rader JA, van Schie RC, LaFace DM, Green DR.

(16) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosisBloodSeptember 1, 1994Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH.

(17) Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophagesJournal of immunology (Baltimore, Md. : 1950)April 1, 1992Fadok VA, Voelker DR, Campbell PA, Cohen JJ, Bratton DL, Henson PM.

(18) Transbilayer movement of phospholipids in red cell and platelet membranesBiochimica et biophysica actaNovember 13, 1991Schroit AJ, Zwaal RF.

(19) Binding of annexin V/placental anticoagulant protein I to platelets. Evidence for phosphatidylserine exposure in the procoagulant response of activated plateletsThe Journal of biological chemistryOctober 15, 1990Thiagarajan P, Tait JF.

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Annexin V-FITC from Miltenyi Biotec

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Annexin V-FITC from Miltenyi Biotec

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