Active Heparanase/HPSE Protein 7570-GH-005 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemActive Heparanase/HPSE Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $588.00Supplier Page View Company Product Page
Catalog Number7570-GH-005
Size5 ug
Molecule NameHeparanase/HPSE
SpeciesHuman
Source/Expression SystemCHO-derived Recombinant Human Active Heparanase/HPSE Protein, CF
FormatSupplied as a 0.2 µm filtered solution in Tris, NaCl and E64
Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
ApplicationsEnzyme Activity
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening
NCBI Full Gene Nameheparanase
NCBI Gene AliasesHPA, HPA1, HPR1, HPSE1, HSE1

Specifications/Features

Available conjugates: Sizes Available: 5 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Netrin-1 inhibits the attachment and internalization of Hepatitis B virus for hepatocyte infectionPLoS PathogDecember 17, 2025Ying Wang, Kazuhisa Murai, Atsuya Ishida, Narumi Kawasaki, Kazuyuki Kuroki, Ying-Yi Li, Yuga Sato, Yutaro Miura, Kureha Takara, Lianghao Kong, et al.anti-DDDDK-tag mAb-Alexa Fluor 594 (M185-A59). Heparanase treatment Recombinant Human Active Heparanase (7570-GH-005; R&D Systems, Minneapolis, MN) at a final concentration of 0.5 µg/mL was added to the cells followed

(2) Matrix metalloproteinase-9 mediates endothelial glycocalyx degradation and correlates with severity of hemorrhagic fever with renal syndromeiScienceSeptember 19, 2025Jacquet C, Gustafsson R, Patel AK, Hansson M, Rankin G, Bano F, Byström JW, Blomberg A, Rasmuson J, Satchell S, et al.the endothelial barrier. Enzymes and recombinant proteins Recombinant human heparanase (rhHPSE; #7570-GH-005, R&D Systems, Sweden) was diluted in rhHPSE assay buffer comprising 20 mM Tris-HCl and 4 mM CaCl2 (pH

(3) Could Hydrophobicity of Sulfated Pseudo-Trisaccharides Derived from Repurposing Aminoglycoside Tobramycin Modulate the Enzymatic Activity of Heparanase?Journal of medicinal chemistryJune 26, 2025Hotor M, Wakpal J, Effah SY, Alom NE, Walker AR, Nguyen HM.33.8, 33.2, 27.6. TR-FRET Assay. Recombinant human active heparanase/HPSE protein, CF (Catalog #: 7570-GH-005, R&D Systems), tris-buffer (contains 20 mM TrisHCl, 0.15 M NaCl, and 0.1% CHAPS, pH 7.5), biotin-heparan

(4) A Screening Approach to Assess the Impact of Various Commercial Sources of Crude Marine λ-Carrageenan on the Production of Oligosaccharides with Anti-heparanase and Anti-migratory ActivitiesMarine drugsMay 11, 2023Manseur C, Groult H, Porta M, Bodet PE, Mersni-Achour R, Petit R, Ali-Moussa S, Musnier B, Le Cerf D, Varacavoudin T, et al.

(5) Heparanase-2 protein and peptides have a protective effect on experimental glomerulonephritis and diabetic nephropathyFrontiers in PharmacologyApril 27, 2023Baranca Buijsers, Marjolein Garsen, Mark de Graaf, Marinka Bakker-van Bebber, Chunming Guo, Xue Li, Johan van der Vlagwas optimized by the use of recombinant active human HPSE1 (Bio-techne, Abingdon, United Kingdom, Cat#7570-GH-005). The in-house developed activity assay was performed as described previously (Buijsers et al., 2020).

(6) Glycosaminoglycans and fucoidan have a protective effect on experimental glomerulonephritisFrontiers in molecular biosciencesJanuary 1, 2023Buijsers B, Maciej-Hulme M, Jacobs M, Bebber MB, de Graaf M, Salmenov R, Parr N, Rabelink TJ, Nijenhuis T, van der Vlag J.developed HPSE activity assay, which was validated by the use of recombinant active human HPSE (Cat#7570-GH-005, Bio-techne), and performed as described previously (Buijsers et al., 2020a). Briefly, Nunc maxisorp

(7) Chemoenzymatic synthesis of sulfur-linked sugar polymers as heparanase inhibitorsNature communicationsDecember 2, 2022He P, Zhang X, Xia K, Green DE, Baytas S, Xu Y, Pham T, Liu J, Zhang F, Almond A, et al.

(8) Increased Heparanase Levels in Urine during Acute Puumala Orthohantavirus Infection Are Associated with Disease SeverityVirusesFebruary 22, 2022Cabrera LE, Schmotz C, Saleem MA, Lehtonen S, Vapalahti O, Vaheri A, Mäkelä S, Mustonen J, Strandin T.cultures, was compared to a standard curve of recombinant human HPSE (Bio–techne; Abingdon, UK, Cat#7570–GH–005). Urine concentrations measured were then normalized by dividing the values by creatinine (heparanase:creatinine

(9) A Marine λ-Oligocarrageenan Inhibits Migratory and Invasive Ability of MDA-MB-231 Human Breast Cancer Cells through Actions on Heparanase Metabolism and MMP-14/MMP-2 AxisMarine drugsSeptember 28, 2021Cousin R, Groult H, Manseur C, Ferru-Clément R, Gani M, Havret R, Toucheteau C, Prunier G, Colin B, Morel F, et al.

(10) COVID-19 generates hyaluronan fragments that directly induce endothelial barrier dysfunctionJCI insightSeptember 8, 2021Queisser KA, Mellema RA, Middleton EA, Portier I, Manne BK, Denorme F, Beswick EJ, Rondina MT, Campbell RA, Petrey AC.

(11) A GPC2 antibody-drug conjugate is efficacious against neuroblastoma and small-cell lung cancer via binding a conformational epitopeCell reports. MedicineJuly 20, 2021Raman S, Buongervino SN, Lane MV, Zhelev DV, Zhu Z, Cui H, Martinez B, Martinez D, Wang Y, Upton K, et al.and NB-1643 cell lines were treated with 150 ng/mL recombinant human active heparanase (R&D Systems, 7570-GH-005) for 2 or 18 hours at which point cells were analyzed by western blot and stained with the D3-GPC2-IgG1-PE

(12) Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?BiomoleculesJanuary 13, 2021Garcia J, McCarthy HS, Kuiper JH, Melrose J, Roberts S.

(13) Ischemic stroke disrupts the endothelial glycocalyx through activation of proHPSE via acrolein exposureThe Journal of biological chemistryDecember 25, 2020Ko K, Suzuki T, Ishikawa R, Hattori N, Ito R, Umehara K, Furihata T, Dohmae N, Linhardt RJ, Igarashi K, et al.

(14) Increased Plasma Heparanase Activity in COVID-19 PatientsFrontiers in ImmunologyOctober 6, 2020Baranca Buijsers et al.was optimized by the use of recombinant active human HPSE (Bio-techne, Abingdon, United Kingdom, Cat#7570-GH-005). In detail, Nunc maxisorp ﬂat bottom 96 plates (Thermo scientiﬁc, Breda, Netherlands) were coated with

(15) Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic micePloS oneJanuary 1, 2020Njau F, Shushakova N, Schenk H, Wulfmeyer VC, Bollin R, Menne J, Haller H.

(16) Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signallingScientific reportsSeptember 19, 2019Kiyan Y, Tkachuk S, Kurselis K, Shushakova N, Stahl K, Dawodu D, Kiyan R, Chichkov B, Haller H.

(17) A Robust, One-step FRET Assay for Human HeparanaseBIO-PROTOCOLSeptember 5, 2019Jyothi C Sistla, Umesh R Desai(DMEM) growth media (Invitrogen, USA) 29. (Optional) Heparanase protein (R&D systems, catalog number: 7570-GH-005) 30. Antibiotic-antimycotic Solution (AA) (Fisher Scientific, catalog number: 15-240-062), antibiotic spectrum:

(18) Imaging specific cellular glycan structures using glycosyltransferases via click chemistryGlycobiologyFebruary 1, 2018Wu ZL, Person AD, Anderson M, Burroughs B, Tatge T, Khatri K, Zou Y, Wang L, Geders T, Zaia J, et al.

(19) Production of heparin and λ-carrageenan anti-heparanase derivatives using a combination of physicochemical depolymerization and glycol splittingCarbohydrate polymersJune 15, 2017Poupard N, Groult H, Bodin J, Bridiau N, Bordenave-Juchereau S, Sannier F, Piot JM, Fruitier-Arnaudin I, Maugard T.

(20) Loss of VEGFB and its signaling in the diabetic heart is associated with increased cell death signalingAmerican journal of physiology. Heart and circulatory physiologyJune 1, 2017Lal N, Chiu AP, Wang F, Zhang D, Jia J, Wan A, Vlodavsky I, Hussein B, Rodrigues B.heparanase (Hep-L) was prepared as previously described (47), recombinant human active heparanase (7570-GH-005) was bought from R&D Systems, and heparin (1,000 U/ml Hepalean) was purchased from Organon. For Western blots

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Recombinant Human Active Heparanase/HPSE Protein, CF

Active Heparanase/HPSE Protein

Active Heparanase/HPSE Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (23)

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Active Heparanase/HPSE Protein from R&D Systems, a Bio-Techne Brand

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