Fig 1: (A) Immunofluorescence staining of TUNEL in lung tissue (x200). (A1) Mean fluorescence intensity of TUNEL (n = 6 per group). (B) Western blot analysis of NOX4, p‐Nrf2, Nrf2, CAT‐1, HO‐1, and β‐actin protein in lung tissue. (B1‐B4) Relative expression of NOX4, HO‐1, CAT‐1, and p‐Nrf2/Nrf2 (n = 3 per group). (C) Western blot analysis of Bax, Bcl‐2, Fas, FasL, Cleaved caspase‐3, caspase‐3, and β‐actin protein in lung tissue. (C1‐C4) Relative expression of Bax/Bcl‐2, Fas, FasL, and Cleaved caspase‐3 (n = 3 per group). These data are expressed as mean ± SD, # p < .05, ## p < .01, compared with the CON group, *p < .05, **p < .01 compared with the BLM group. Bax, Bcl‐2‐associated X protein; Bcl‐2, B cell lymphoma/leukemia‐2; CAT‐1, catalase‐1; HO‐1, Heme Oxygenase‐1; NOX4, NADPH Oxidase 4.
Fig 2: (A−C) Levels of TGF‐β1 and E‐cadherin in lung tissue and IL‐6 in BALF (n = 6 per group). (E−F) Levels of T‐SOD, MDA, and GSH‐Px in mice lung tissue (n = 6 per group). (G−L) Real‐time qPCR analysis of Vimentin, HO‐1, Bcl‐2, Bax, NOX4, and CAT‐1 (n = 3 per group). These data are expressed as mean ± SD, # p < .05, ## p < .01, compared with the CON group, *p < .05, **p < .01 compared with the BLM group. BALF, bronchoalveolar lavage fluid; Bax, Bcl‐2‐associated X protein; Bcl‐2, B cell lymphoma/leukemia‐2; CAT‐1, catalase‐1; HO‐1, Heme Oxygenase‐1; NOX4, NADPH Oxidase 4. GSH‐Px, glutathione peroxidase; MDA, malondialdehyde; TGF‐β1, transforming growth factor beta‐1; T‐SOD, total superoxide dismutase.
Supplier Page from Abcam for Recombinant Human CAT1 protein (GST tag N-Terminus)