APC Annexin V 640920 from BioLegend

Product Specs
ItemAPC Annexin V
CompanyBioLegend
Price $220.00Supplier Page View Company Product Page
Catalog Number640920
Size100 tests
ApplicationsFC - Quality tested
Conjugate/TagAPC
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Questions and Answers

Can this APC Annexin V be used to assess apoptosis in T lymphocytes?

Yes, the APC Annexin V has been utilized in studies to assess apoptosis in T lymphocytes. For instance, in one study, cells were stained with APC-conjugated Annexin V and analyzed by flow cytometry to evaluate apoptosis in activated T cells ().

Is the APC Annexin V effective for detecting apoptosis in human cells?

The APC Annexin V has been shown to work effectively in human cells, as evidenced by its use in various studies involving human cell lines. For example, it was employed in an apoptosis assay involving human cervical cancer cells and T-lymphocytes, demonstrating its applicability in human cell models ().

What is the recommended method for using APC Annexin V in flow cytometry?

The recommended method involves staining cells with APC Annexin V in conjunction with an Annexin V Binding Buffer, followed by analysis using flow cytometry. This approach allows for the accurate detection of apoptotic cells, as demonstrated in multiple studies (; ).

Are there specific conditions or treatments that enhance the effectiveness of APC Annexin V in apoptosis assays?

Yes, the effectiveness of APC Annexin V can be enhanced by specific treatments that induce apoptosis, such as the use of staurosporine or anti-Fas antibodies. These treatments have been shown to increase the detection of apoptotic cells when using Annexin V staining in flow cytometry assays (; ).

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Company Profile

Website: biolegend.com

(1) Translation factor eIF4G2 directs CD8+ T cell lineage commitment by selectively enabling the IL-7 receptor responseiScienceApril 17, 2026Jialong Cui, Xinhui Zhang, Yang Yang, Lidong Shan, Yang Li, Long Jiang, Wei Xie, Tengchuan Jin, Xueting Lang100X",Beyotime,Cat# C0222 DMEM (High Glucose) Medium,Biochannel,Cat# BC-M-005 "Annexin V, APC",BioLegend,Cat# 640920 TRIzol Reagent,Invitrogen,Cat# AM9738 Trypan Blue solution,Sigma,Cat# 72-57-1 ,, Critical commercial

(2) Fufang Huangbo Formula mitigates myeloproliferative neoplasms by activating p53/p21 signaling axis and inhibiting STAT3 and NF-κB signaling pathwaysPharm Sci AdvApril 16, 2026Mingjie Liu, Yanxia Li, Chengxue Qin, Lingling Wang, Meiqi Guo, Yi Wang, Qian Zhou, Zhida Shi, Weimin Hao, Yuan Li, et al.analysis, the cells were cultured with FHF for 24 h, followed by 15 min staining with Annexin V (Biolegend, 640920), and added propidium iodide (PI, 50 μg/mL), with subsequent flow cytometry analysis. For cell cycle,

(3) Chemoradiotherapy-Integrated Tumor Cell-Derived Microparticles Mediate Tumor Eradication in Malignant Pleural EffusionJ Extracell VesiclesApril 7, 2026Minqi Zhou, Lingyi Kong, Weidong Zhong, Yijun Wang, Wenqian Yuan, Xixi Liu, Jiacheng Wang, Mengyao Su, Yuze Zheng, Zishan Feng, et al.treatment with CR‐MPs, RT‐MPs, or chemical agents, cells were harvested, stained with Annexin V/7‐AAD (640920/640934, Biolegend) according to the manufacturer's protocol, and incubated at 4°C for 30 min before

(4) Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor GrowthACS NanoMarch 31, 2026Patricia Ines Back, Shadan Modaresahmadi, Jalpa Patel, Indhumathy Subramaniyan, Vindhya Edpuganti, Md. Rakibul Islam, Seonjuti Chowdhury, Adam J. Carr, Qisheng Zhang, William C. Putnam, et al.(catalog no. 422201, BioLegend). Approximately 1 × 106 cells were stained with APC Annexin V (Cat. #640920, BioLegend) and propidium iodide (Cat. #421301, BioLegend). Apoptosis and necrosis controls were used

(5) MHC class I on target cells regulates CD4+ T cell-mediated immunityNature ImmunologyMarch 24, 2026Emma Lauder, Mahnoor Gondal, Meng-Chih Wu, Akira Yamamoto, Laure Maneix, Dongchang Zhao, Yaping Sun, Marcin Cieslik, Arul M. Chinnaiyan, Pavan ReddyBiolegend, 118208), anti-CD326–BV711 (G8.8, 1:200; Biolegend, 118233), anti-annexin V–APC (1:20; Biolegend, 640920), anti-H2kb–BV421 (AF6-88.5, 1:100; Biolegend, 116525), anti-IA/IE–APC (M5/114.15.2, 1:100; Biolegend,

(6) Fine tuning of TCR signaling via CD8αβ and PD-1 and the fate of autoreactive thymocytes during negative selectionImmunohorizonsMarch 10, 2026Martha C Zúñiga, Sangwon Hyun, Jacob Du, Shahar Dubiner, Alicia Freedman-Goretsky, Yitzhar Goretsky, Anita Pothukuchi, Nicholas A Y Fong, Alexander Berg, Caitlin Davis, et al.CD5,BioLegend,100644/100UG AF488 anti-mouse IgG,A11017,Invitrogen 7-AAD,420403,BioLegend APC Annexin V,640920,BioLegend Biotin anti-mouse CD45.2,109803,BioLegend Biotin anti-mouse CD5,100603,BioLegend PE/DazzleTM

(7) PD-1 protects expanding human T cells from premature restimulation-induced cell death by modulating TCR and CD28 signalingCell Death & DiseaseFebruary 26, 2026Katherine P. Lee, Sara Elster, Benjamin Epstein, Camille M. Lake, Andrew L. Snowpolypropylene tubes with 2 mL FACS buffer. Cells were then stained with 1.5 μl of Annexin V APC (Biolegend, #640920) in 200 μL of Annexin V Binding Buffer (Biolegend, #422201) for 15 min at room temperature (RT) in the

(8) Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelinationiScienceFebruary 20, 2026Weixing Yan, Dong Xiang, Li Du, Di Zhu, Qi Jia, Yuting Liu, Siyu Wang, Li Liu, Haihao Guan, Yelin Zhao, et al.with antibodies against microglia markers (CD45loCx3cr1+CD11b+) and APC-Annexin V (BioLegend, Cat: 640920) diluted in 1 × Annexin-V Binding Buffer for 30 min, followed by washing with 1 × Annexin-V Binding Buffer

(9) Avoidance of related donors in CAEBV with germline immune variants: long-termoutcome of matched unrelated donor HSCT - a case reportAnnals of HematologyFebruary 19, 2026Xinran Wang, Jia Gu, Ning An, Qiuxia Yu, Yuhan Bao, Yang Gao, Jianlin Hu, Hui Luo, Chunrui Lieffector-to-target (E: T) ratio for 4 h. Target cell apoptosis was assessed using Annexin V-APC (BioLegend, Cat# 640920) and propidium iodide (Sigma, Cat# P4170) staining. During flow cytometric analysis, K562 target cells

(10) Synergistic mechanical and therapeutic modulation of engineered tumor cell-derived microparticles for enhanced cancer treatmentCell Reports MedicineFebruary 17, 2026Xiaojuan Zhang, Ke Zhang, Yinmei Zhu, Shiyi Xu, Shiyu Li, Haojie Liu, Weilin Lv, Zixiang Xie, Yizhou Huang, Xin Li, et al.GB12135 FITC-anti-human CD45,Biolegend,Cat# 304006; RRID: AB_314394 APC-anti-human Annexin V,Biolegend,Cat# 640920 Anti-SOX2 antibody,Servicebio,Cat# GB15249 Anti-flotillin-1 antibody,ABclonal,Cat# A3023; RRID: AB_2863023 ,, Biological

(11) Proteomics landscape of early T-cell precursor acute lymphoblastic leukemia reveals deficient oxidative phosphorylation signaturesCell Reports MedicineFebruary 17, 2026Miaomiao Liu, Tianyi Chen, Xiangjie Lin, Bowen Wu, Fujie Shi, Junya Liu, Yi Chen, Tao Zeng, Shangyu Hou, Qingfei Pan, et al.type or cell line individually. Apoptosis was assessed based on Annexin V-APC (Biolegend, USA, Cat#640920)/DAPI (Biolegend, USA,Cat#422801) staining on CytoFLEX LX (Beckman). Cytochrome c oxidase assay Cytochrome

(12) LMO2 Regulates Epithelial-Mesenchymal Plasticity of Mammary Epithelial CellsJournal of Mammary Gland Biology and NeoplasiaFebruary 9, 2026Veronica Haro Acosta, Andrew Olander, Isobel J. Fetter, Maria A. Juarez, Shaheen S. Sikandar(Invitrogen: BDB556454) at a concentration of 1 mL per 1 × 10⁶ cells. APC-Annexin V antibody (BioLegend: 640920) was added at 5 µL per 100 µL of 1× binding buffer. Cells were incubated with the antibody for 15 min,

(13) Recombinant Dabie bandavirus as a bivalent vaccine platform inducing protective immunity against intracellular pathogens and cancerMolecular TherapyFebruary 4, 2026Hyo-Jin Ro, Yebeen Lee, Kyeongseok Jeon, Yujin Kim, Seung Ho Baek, Green Kim, Joowan Kim, Jun-Gu Kang, Na-Yoon Jang, Si-Hyeon Lee, et al.WT virus at an MOI of 1 and incubated for 24 or 48 h. Cells were stained with Annexin V (BioLegend #640920) in Annexin V binding buffer (140 mM NaCl and 2.5 mM CaCl2) for 15 min at RT in the dark, followed by

(14) Glutamine-driven upregulation of NPDC1 promotes colorectal cancer progression through PI3K/AKT signalingJournal of Translational MedicineJanuary 24, 2026Qing Qin, Dapeng Zhang, Yusai Xie, Fan Zhang, Yulan Huang, Renchao Deng, Minli Yang, Lili Zhang, Houjie Liang, Rongchen Shieach well (1 × 10⁶/ml) and processed according to the instructions of the Annexin V/PI staining kit (#640920, #422201, BioLegend), followed by analysis via flow cytometry (BD FACSVerse C6, USA) and FlowJo 10.8.1

(15) The Taurine-Slc6a6 Axis Promotes Breast Cancer Progression by Alleviating Oxidative Stress and Accelerating Cell Cycle ProgressionCellsJanuary 22, 2026Pei Cai, Xiaoqin Liu, Meizhen Lin, Shaofang Xie, Lei Yuan, Zuobao Lin, Yue Zhang, Shang Caisoftware V10.8.1. 2.16. Annexin V Test Primary tumor cells were stained with APC-annexin V (BioLegend, 640920, 1:200) according to the manufacturer’s instructions. Approximately 5 × 105 cells were seeded in 6-well

(16) Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftmentNature CommunicationsJanuary 10, 2026Julian Boutin, Sabrina Fayet, Victor Marin, Camille Bergès, Maude Riandière, Jérôme Toutain, Isabelle Lamrissi-Garcia, Chloé Thibault, David Cappellen, Sandrine Dabernat, et al.manufacturer’s instructions. Apoptosis was detected by staining the cells with anti-human APC Annexin V (#640920 Biolegend, San Diego, CA, USA) and propidium Iodide (#596552, Dutscher) solution followed by flow cytometry analysis

(17) Telomerase mRNA therapy protects human skin against radiation-induced DNA damageMolecular TherapyJanuary 7, 2026Shuang Li, David F. Chang, Karem A. Court, Thi Kim Cuc Nguyen, Vrutant V. Shah, Elisa Morales, Jack Carrier, Anjana Tiwari, Andrew T. Ludlow, Kristopher W. Brannan, et al.Annexin V binding buffer (BioLegend, catalog no. 422201), and incubated with APC Annexin V (BioLegend, catalog no. 640920) for 15 min at room temperature in the dark. The samples were then analyzed using an LSR II or Fortessa

(18) Demethylzeylasteral alleviates myeloid leukemia through PERK/eIF2α/ATF4/CHOP-mediated cell apoptosisTranslational Cancer ResearchDecember 31, 2025Xue Li, Xianchao He, Leyan Zhang, Zhigang Li, Yuanyuan LiuCell Signaling Technology, Danvers, USA). For flow cytometry, the antibodies used including anti-annexinV-APC (#640920, Biolegend, San Diego, USA), 4',6-diamidino-2-phenylindole (DAPI; #C1002, Beyotime), anti-Ki67-PE (#151210,

(19) MYC inhibition by Omomyc causes DNA damage and overcomes PARPi resistance in breast cancerCell ReportsDecember 23, 2025Fabio Giuntini, Íñigo González-Larreategui, Andrea Herencia-Ropero, Silvia Casacuberta-Serra, Mariano F. Zacarías-Fluck, Magdalena Arnal, Flaminia Pedretti, Sandra Martínez-Martín, Hugo Thabussot, Virginia Castillo Cano, et al.anti-rabbit secondary antibody,Invitrogen,Cat# A-11011; RRID:AB_143157 Annexin V-APC,Biolegend,Cat# 640920 ,, Biological samples,Biological samples,Biological samples ,, FFPE HR-altered metastatic breast cancer

(20) Dynamic release of extracellular particles after opening of the blood-brain barrier predicts glioblastoma susceptibility to paclitaxelNat CommunDecember 16, 2025Mark W. Youngblood, Abha Kumari, Yoon-Tae Kang, Andrew Gould, Karl Habashy, Mateo Gomez, Harika Lingamarla, Trevor Morey, Li Chen, Harrshavasan Congivaram, et al.to detach cells. Apoptotic cells were then stained using APC-conjugated Annexin-V (Biolegend Cat. #640920) and Propidium Iodide (PI, Invitrogen Cat. # 00-6990-50) following the manufacturer’s protocol. Cell

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(1)

8/6/2018Annexin V APC for Determining Apoptosis in Cells Suzanne TomchuckWe often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the... Read Review
(1)

2/24/2017Staining T Cells With Annexin V APC Adam GuessThis reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other ... Read Review

APC Annexin V from BioLegend

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APC Annexin V from BioLegend

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