DAPI from BioLegend

Product Specs
ItemDAPI
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number422801
Quantity10 mg
ApplicationsICFC, ICC
FormatLyophilized
TargetDAPI

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Enhanced efficacy of gastric cancer treatment by a combination of NK cells and chemotherapyiScienceJune 19, 2026Jingjing Yue, Jie Ma, Yangyang Li, Hongbo Dong, Yongge He, Minhua Chen, Fang Fang, Weihua XiaoC-5138 DNASE I,Sigma,Cat# C-5025 "D-Luciferin,Potassium Salt",GOLDBIO,Cat# 115144-35-9 DAPI,Biolegend,Cat# 422801 Mouse serum,Future,Cat# F001008 "1,1′-dioctadecyl-3,3,3′,3′-tetramethy lindocarbocyanine perchlorate",Thermo

(2) Topical delivery of a PPARγ agonist enables adipogenic repair of irradiated skinMolecular Therapy AdvancesJune 11, 2026Ashley Siegel, Samerender Hanumantharao, Pratik Koirala, Viren Sehgal, Chaoqi He, Menfang Wu, Soheila Ali Akbari Ghavimi, Ziyu Chen, Zachary Williams, Xi Xu, et al.(Invitrogen #A32731, 1:500; AAT Bioquest #16837, 1:300), and counterstained with DAPI (BioLegend, #422801, 1:500). Images were acquired at 40× using an Olympus Fluoview confocal microscope with standardized

(3) Taxane chemotherapy promotes response to TIM-3 checkpoint blockade via STING-mediated ER stress and HMGB1 secretionCell Rep MedMay 19, 2026Alexis Onimus, Daiana Celias, Shiun Chang, Joshua Davis, Jay Mandula, Jie Li, Alycia Gardner, Kay Hänggi, Olabisi Osunmakinde, Hatem Soliman, et al.Albumin,FisherScientific,Cat# BP1600-100 "DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate)",Biolegend,Cat# 422801 DMEM high glucose (4.5g/L),Thermo Fisher,Cat# 11965118 DMSO,FisherScientific,Cat# BP231-100 DMXAA,Invivogen,Cat#

(4) Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodelingSignal Transduct Target TherMay 19, 2026Huimin Li, Yabin Liu, Jiachen Cui, Ziyu Fu, Yiyang Xu, Sizhe Yang, Kai Zhu, Yuhao Xue, Yi Zhang, Yiheng Zhao, et al.(Biolegend, 366620), anti-CD45-APC-cy7 (Biolegend, 368515), and nuclear fluorescent DAPI (Biolegend, 422801). Stained BMOs were embedded in double-layer 0.5% low-melting agarose (Yeasen, 10214ES08), dehydrated

(5) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.Millipore Sigma, # P4170), 7-AAD (1 μg ml–1, Millipore Sigma, #A9400) or DAPI (0.5 μg ml–1, BioLegend, #422801) was added as a nuclear counterstain. For live cell staining without cell permeabilization, after cell

(6) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.Millipore Sigma, # P4170), 7-AAD (1 μg ml–1, Millipore Sigma, #A9400) or DAPI (0.5 μg ml–1, BioLegend, #422801) was added as a nuclear counterstain. For live cell staining without cell permeabilization, after cell

(7) Foundation cell segmentation models performance on live microscopy and spatial-omics databioRxivApril 22, 2026Yang Miao, Nick Surguladze, Josh Lerner, Koravit Poysungnoen, Ky Ariano, Yuexi Li, Yining Zhu, Kyra Van Batavia, Jodie Jepson, Josie Van De Klashorst, et al.dark. Subsequently, nuclei were stained with DAPI at a concentration of 16.7 μg/mL (BioLegend, cat# 422801). Fluorescence images were acquired using the Squid microscope27 (Cephla Inc.) with a 20x/0.8NA objective

(8) Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral NeuropathyCellsApril 19, 2026Sybil C. L. Hrstka, Maya Jahnke, Kylie Meng-Lin, Sarah Lindorfer, Henry Noma, Ronald F. Hrstka, Nathan P. Staffincubation for 2 h followed the next day, cells were counterstained with DAPI (BioLegend, Catalog No. 422801, San Diego, CA) and mounted with Prolong Gold Antifade Mountant (InvitrogenTM, Catalog No. P36962, Carlsbad,

(9) Creatine uptake promotes dendritic cell activation and enhances antitumor immunityiScienceApril 17, 2026Elliot Kang, James Elsten-Brown, Yu-Chen Wang, Ashley Lam, Elise Sanchez, Renee Wen, Tiffany Wang, Jennifer Chiang, Quentin Scarborough, Yan-Ruide Li, et al.cell culture,Sigma-Aldrich,CAT#M3148 Normocin,Invivogen,CAT#ant-nr-2 "4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI)",BioLegend,CAT#422801 Fixable Viability Dye eFluor506,affymetrix eBioscience,CAT#65-0866-14 Cell Fixation/Permeabilization

(10) Comprehensive Analysis of Neutrophil Immunomodulatory Properties and FcR Dynamics in HIV-1 Infection and TherapyiScienceApril 17, 2026Soledad Marsile-Medun, Manon Souchard, Daouda Abba Abba Moussa, Valérie Lorin, Hugo Mouquet, Elisa Reynaud, Rayane Dibsy, Edouard Tuaillon, Delphine Muriaux, Giang Ngo, et al.eBiosciences #17-1729-42). The viability staining was made by resuspending the cells with 1mL DAPI (Biolegend #422801) diluted at 3μM with an incubation of 15 minutes at room temperature. After the washing of fluorescent

(11) Pelargonium graveolens Essential Oil Suppresses Proliferation and Migration and Modulates Mesenchymal-Associated Cellular Functions in Human Endometriotic CellsCellsApril 15, 2026Elif Karakoç, Sezai Berkand Koçak, Kevser Kişifli Köş, Hülya Kayhan, Eda Erdem Şahinkesen, Cemil Can Eylem, Ferda Topal Çelikkan, Emirhan Nemutlu, Pergin Atillapurchased from Abcam (Cambridge, UK). Anti-CD90 (328102), anti-CD73 (344002), anti-CD105 (800501), DAPI (422801), and Annexin V-FITC/Propidium Iodide (PI) apoptosis detection kit (640914) were from Biolegend (San

(12) L-RNA aptamer-based CXCL12 inhibition combined with radiotherapy and bevacizumab in newly-diagnosed glioblastoma: expansion of the phase I/II GLORIA trialNat CommunApril 8, 2026Frank A. Giordano, Julian P. Layer, Roberta Turiello, Lea L. Friker, Oriol Mirallas, Barbara E. F. Pregler, Anna-Laura Potthoff, Thomas Zeyen, Johannes Weller, Elena Sperk, et al.0.02% w/v NaN3 in ddH2O." Plate buffer,"H2 buffer containing DAPI nuclear stain (1:300, Biolegend, 422801) and 0.5 mg/ml sheared salmon sperm DNA. Fluorescent oligonucleotide stock solution (Biomers): 100 µM

(13) A 3D microfluidic model of exchange between perfused blood and lymphatic microvascular networksLab on a ChipApril 7, 2026Delaney Gray-Scherr, Terry Ching, Katherine Beran, Sarah C. Adams, Emily Davis, Abraham C. I. van Steen, Trisha Raman, Wilson W. Wong, Jeroen Eyckmans, Christopher S. ChenFluor Plus 555 (Invitrogen, A32732). Nuclear and cytoskeletal counterstaining used DAPI (BioLegend, 422801), Alexa Fluor Plus 647 Phalloidin (Thermo Fisher, A30107), UEA I DyLight 649 (Vector Laboratories, DL-1068-1),

(14) LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasisiScienceMarch 20, 2026Ke-En Tan, Zuag Paj Her, Cheng Zhang, Kok Siong Yeo, Taylor Weiskittel, Cassie Howe, Shyang Hong Tan, Katherine M. Johnson, Gina L. Razidlo, Thomas E. Rubino, et al.CO2 at 37°C for 4-6 h and fixed with 4% paraformaldehyde. Nuclei were stained with DAPI (Biolegend, #422801). Coverslips were imaged with Nikon Ti2-E widefield fluorescent microscope. Areas of gelatin degradation

(15) FGFR signaling establishes spatial gradients of secretory cell identities along the airway proximal-distal axisNat CommunMarch 18, 2026Alexandros Sountoulidis, Jonas Theelke, Andreas Liontos, Alexandra B. Firsova, Orane Eliot, Janine Koepke, Pamela Millar-Büchner, Louise Mannerås-Holm, Åsa Björklund, Athanasios Fysikopoulos, et al.at RT for 1 h in the dark. The nuclei were counterstained with a DAPI solution 0.5 µg/µl (Biolegend, 422801) in PBS 1X Triton-X100 0.1%, and for mounting we used the ProLong Gold Antifade Reagent (Thermo-Fischer Scientific

(16) O-GlcNAcylation regulates PPAR-driven metabolic programming in intestinal stem cellsbioRxivMarch 16, 2026Thomas Hartley Hartley McDermott, Dominic R Saiz, Yesenia Barrera Barrera Millan, Ngoc Bao Phuong Phuong Ho, Matthew Torel, Eric Uher, Caleb Aboagye, Fiona Farnsworth, Gourab Lahiri, Venkataramana Thiriveedi, et al.through a 70μm mesh cells were incubated with EpCAM-APC (Bioscience, 17–5791-81) and DAPI (BioLegend, 422801) was used to exclude dead cells from analysis. ISCs were isolated as Lgr5-eGFPhiEpCAM+ cells and TA

(17) Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infectionNature ImmunologyMarch 4, 2026Weixiang Liu, Jieming Ping, Lishan Deng, Ke Wang, Jinnuo Xu, Ningxin Peng, Mengyun Yang, Hesen Tang, Zhengjie Liu, Wenliang Wang, et al.containing annexin V (5 ng ml−1) and either propidium iodide (100 ng ml−1, P4170, Sigma) or DAPI (40 nM, 422801, BioLegend). The samples were then analyzed using either a LSR Fortessa or a Symphony A5 flow cytometer

(18) Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilienceJournal of Clinical InvestigationMarch 2, 2026Myung Jin Yang, Seok Kang, Seon Pyo Hong, Hokyung Jin, Jin-Hui Yoon, Cheolhwa Jin, Chae Min Yuk, Lydia Getachew Gebeyehu, Junho Jung, Sung-Hwan Yoon, et al.the DC medium for 24 h. The purity of differentiated DC was assessed by flow cytometry using DAPI (422801, BioLegend), anti-mouse CD45-BV711 (103147, BioLegend), anti-mouse CD11c-PE (117308, BioLegend), and

(19) Stepwise assembly of α-hemolysin from intermediates to the mature pore in native erythrocytesJournal of Cell BiologyMarch 2, 2026Arnab Chatterjee, Anupam Roy, Partho Pratim Das, Debajyoti Chakraborty, Bartika Ghoshal, Siddharth Jhunjhunwala, Somnath Duttapresence of lipophilic membrane dye Nile red (Cat#72485; Sigma-Aldrich) and DNA staining dye DAPI (Cat#422801; BioLegend). An excitation laser source at 405 and 540 nm was used for DAPI and Nile red fluorescence imaging

(20) TGF ‐β Inhibitor Potentiates Osimertinib‐Induced Anti‐Tumor Immunity in Egfr ‐Mutant Lung CancerCancer ScienceFebruary 27, 2026Tadahiro Kuribayashi, Jun Nishimura, Sachi Okawa, Masataka Taoka, Shunta Mori, Takaaki Tanaka, Tomoka Nishimura, Ayako Morita, Naofumi Hara, Kiichiro Ninomiya, et al.BSA (Sigma‐Aldrich; 10735078001) in PBS and incubated with mAbs for 30 min at 4°C. DAPI (Biolegend; 422801) was used to assess cell viability. For CD107a analysis, freshly isolated tumor‐infiltrating lymphocytes

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(1)

3/28/2023Great DAPI for Immunocytochemistry Umut TankI use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really low. So, it will last forever.
(1)

7/12/2022DAPI Nuclear Stain Matthew RatsepI use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.

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DAPI from BioLegend

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