DAPI from BioLegend

Product Specs
ItemDAPI
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number422801
Quantity10 mg
ApplicationsICFC, ICC
FormatLyophilized
TargetDAPI

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Simultaneous gene engineering of T cells with multiple neoantigen-specific T cell receptors for adoptive cell transferMol Ther AdvSeptember 10, 2026Melinda Magna, Lior Levy, Krisztian Csomos, Aaron J. Dinerman, Abraham A. Hakim, Noam Levin, Sanghyun P. Kim, Nikolaos Zacharakis, Neilesh B. Parikh, Brian D. Bui, et al.anti-mouse TCR-β FITC (BD #553171) propidium iodide (#P1304MP, Thermo Fisher), or DAPI (BioLegend, #422801). Phenotyping panel: live/dead stain (eBioscience fixable viability dye eFluor 780, #65-0865-14), CD3-BUV395

(2) Protocol for generation of a 3D humanized lung-tumor-on-a-chip model of immune-hot and immune-cold microenvironmentsSTAR ProtocJuly 23, 2026Sabrina Brito Añez, Sanne van Kesteren, Peter Carmeliet, Anna Rita Cantelmo, Patricia Yagüe, Jeffrey Kroon,Cell Signaling Technology,8953 Mouse anti-human CD31 Alexa Fluor 647 1:200,BD Pharmingen,561654 DAPI 1:5000,BioLegend,422801 Hoechst 1:500,Thermo Fischer Scientific,62249 ,, Biological samples,Biological samples,Biological

(3) Emergency myelopoiesis in murine arthritis results in cell-intrinsic changes in dendritic cell development and functioniScienceJuly 17, 2026Lin Tze Tung, Roselyn R. Jiang, Bianca Pozzebon, Mathieu Mancini, Lucie Droin, Mitra Yousefi, Joo Eun (June) Kim, Marwah Mousa, Yue Liang, Connie M. Krawczyk, et al.Scientific,SB-4401-75 Viability Dye eFluor 506,ThermoFisher Scientific,65-0866-14 DAPI,Biolegend,422801 Fc blocker TruStain FcX,Biolegend,101320 Proliferation Dye eFluor 450,ThermoFisher Scientific,65-0842-85 Lipopolysaccharide,Millipore-Sigma,"LPS25,

(4) Eliminating PD‐L1 on Dendritic Cell Extracellular Vesicles for Immunotherapy Potentiates Immune‐Mediated Tumour Rejection in MiceJournal of Extracellular VesiclesJuly 1, 2026Loes Teeuwen, Loïc Steiner, Chantal Reinhardt, Annemarijn Offens, Jesse E. Kuipers, Daniel Martínez‐Martínez, Jules Mazouin, Benedict J. Chambers, Gözde Güçlüler Akpinar, Susanne Gabrielssonco‐cultures were resuspended in 200 µL AnnexinV binding buffer or PBS, respectively, containing 3 µM DAPI (422801, BioLegend). Isolated cells from spleens, tumours and TDLNs were stained and incubated for 30 min at

(5) CD177 Deficiency Defines a Stable Subtype of Human Neutrophil Granulocytes with Tumor Promoting ActivityAdvanced ScienceJune 22, 2026Marcel Jung, Alexander Beer, Susmita Ghosh, Ekaterina Pylaeva, Belal Alshaar, Tobias Tertel, Nils Bastian Leimkühler, Thomas Schroeder, Janine Gronewold, Nina Hagemann, et al.was carried out at RT for 1 h, followed by washing with PBS. DAPI (1:200 in PBS) (BioLegend; Cat. # 422801) staining was performed with an incubation time of 30 min. Fluorescence images were acquired with an

(6) Enhanced efficacy of gastric cancer treatment by a combination of NK cells and chemotherapyiScienceJune 19, 2026Jingjing Yue, Jie Ma, Yangyang Li, Hongbo Dong, Yongge He, Minhua Chen, Fang Fang, Weihua XiaoC-5138 DNASE I,Sigma,Cat# C-5025 "D-Luciferin,Potassium Salt",GOLDBIO,Cat# 115144-35-9 DAPI,Biolegend,Cat# 422801 Mouse serum,Future,Cat# F001008 "1,1′-dioctadecyl-3,3,3′,3′-tetramethy lindocarbocyanine perchlorate",Thermo

(7) An ITGA11 expressing subpopulation as predictor for the donor-specific osteogenic capacity of stromal cellsBone ResJune 15, 2026Ali Jasim Mohammad Jamil, Kaja Madsen, Souad Daamouch, Birgitte Villadsen, Chao Ma, Shakespeare Jeromdesella, Mikkel Ørnfeldt Nørgård, Emilie Grupe, Maria Bruun Jakobsson, Yuting Wang, et al.fluorophores except one, for FACS were used as negative controls. Dead cells were excluded using 1 μg/mL DAPI (422801, BioLegend). Due to a high number of utilized fluorophores, compensation was required to correct for

(8) Topical delivery of a PPARγ agonist enables adipogenic repair of irradiated skinMolecular Therapy AdvancesJune 11, 2026Ashley Siegel, Samerender Hanumantharao, Pratik Koirala, Viren Sehgal, Chaoqi He, Menfang Wu, Soheila Ali Akbari Ghavimi, Ziyu Chen, Zachary Williams, Xi Xu, et al.(Invitrogen #A32731, 1:500; AAT Bioquest #16837, 1:300), and counterstained with DAPI (BioLegend, #422801, 1:500). Images were acquired at 40× using an Olympus Fluoview confocal microscope with standardized

(9) Ultrastructural diversity and subcellular organization of nigral Lewy pathology in Parkinson’s diseaseNature CommunicationsJune 8, 2026Amanda J. Lewis, Lukas van den Heuvel, Marta Di Fabrizio, Kaliya Bandelier, Daria Proniakova, Domenic Burger, Notash Shafiei, Kazadi Ekundayo, Selene Offringa, Evelien Huisman, et al.Molecular Probes A32787 - 1/400, chicken 647 Molecular Probes A32933 - 1/400) and DAPI (Biolegend #422801 - 1/800 dilution) to label cell nuclei after incubation at room-temperature for 30 mins. 3D fluorescent

(10) CCL19⁺ fibroblasts define a proliferative niche in chronic lymphocytic leukemiabioRxivMay 30, 2026Antonio P. Ferreira, Shumei Wang, Larysa Poluben, Dominique Morais, Joshua Brandstadter, Eric Perkey, Steven Sotirakos, Mai Drew, Li Pan, Madeleine E. Lemieux, et al.min with Fc Blocking Reagent (Biolegend, cat. #422302) followed by DAPI (Biolegend, 1 μg/mL, cat. #422801) in FACS buffer (phosphate buffered saline [PBS] containing 2% fetal bovine serum). Cells were subsequently

(11) Limited Immune-Mediated Efficacy of Anti-PD-L1/VEGF in EGFR-TKI-Naïve Egfr-Mutant Lung Cancer with Non-Inflamed Tumor MicroenvironmentCurrent OncologyMay 27, 2026Atsuko Hirabae, Tadahiro Kuribayashi, Shuta Tomida, Sachi Okawa, Takamasa Nakasuka, Kazuya Nishii, Jun Nishimura, Go Makimoto, Kiichiro Ninomiya, Hisao Higo, et al.(FITC, GK1.5, or PE/Cy7, GK1.5) and CD8α (APC/Cy7, 53-6.7) from BioLegend (San Diego, CA, USA). DAPI (#422801) or the Zombie NIR Fixable Viability Kit (#423105) purchased from BioLegend were used for dead cell

(12) Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr ‐mutated lung cancerMolecular OncologyMay 21, 2026Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, et al.monoclonal antibodies against surface markers for 30 min at 4 °C in the FACS staining buffer. DAPI (cat. no. 422801; BioLegend) was used to assess cell viability. The samples were acquired using a MACSQuant flow cytometer

(13) Dual Role of LBH589 in Triple‐Negative Breast Cancer: Inhibition of Tumor Growth and Enhancement of Antitumor ImmunityCancer Rep (Hoboken)May 21, 2026Liang Anjing, Yuan Rong, Xiang Su, Cheng Liang, Hou Jue, Chen Zhustained for T cells and NK cells, including the following antibodies: Pacific Blue‐mouse‐DAPI (Cat. no. 422801, BioLegend, USA), APC‐mouse‐CD3 (Cat. no. 100236, BioLegend, USA), FITC‐mouse‐CD4 (Cat. no. 100406,

(14) Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodelingSignal Transduct Target TherMay 19, 2026Huimin Li, Yabin Liu, Jiachen Cui, Ziyu Fu, Yiyang Xu, Sizhe Yang, Kai Zhu, Yuhao Xue, Yi Zhang, Yiheng Zhao, et al.(Biolegend, 366620), anti-CD45-APC-cy7 (Biolegend, 368515), and nuclear fluorescent DAPI (Biolegend, 422801). Stained BMOs were embedded in double-layer 0.5% low-melting agarose (Yeasen, 10214ES08), dehydrated

(15) Taxane chemotherapy promotes response to TIM-3 checkpoint blockade via STING-mediated ER stress and HMGB1 secretionCell Rep MedMay 19, 2026Alexis Onimus, Daiana Celias, Shiun Chang, Joshua Davis, Jay Mandula, Jie Li, Alycia Gardner, Kay Hänggi, Olabisi Osunmakinde, Hatem Soliman, et al.Albumin,FisherScientific,Cat# BP1600-100 "DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate)",Biolegend,Cat# 422801 DMEM high glucose (4.5g/L),Thermo Fisher,Cat# 11965118 DMSO,FisherScientific,Cat# BP231-100 DMXAA,Invivogen,Cat#

(16) ABHD17C‐Mediated S‐Depalmitoylation of BCL6B Enhances CD24 Transcription to Resist Macrophage Phagocytosis in Pancreatic CancerAdvanced ScienceMay 19, 2026Yalu Zhang, Di Cui, Fanzheng Meng, Xu Zhu, Liang Zhu, Liang Wu, Haifeng Hu, Jizhou Wang, Hanhui Yao, Lianxin Liuwith FCM buffer, and resuspended in buffer containing 1 µg/mL 4’,6‐diamidino‐2‐phenylindole (DAPI; 422801, BioLegend) to exclude dead cells. Data were acquired on an LSRFortessa flow cytometer (BD Biosciences).

(17) Proteostasis sustains T cell differentiation potential and tumor-infiltrating lymphocyte functionCellMay 14, 2026Nicole E. Scharping, Xuezhen Ge, Maria Inês Matias, Fulin Jiang, Allison Cafferata, Maximilian Heeg, Alexander Monell, Giovanni Galletti, Kitty P. Cheung, Angelica Rock, et al.purified protein,UC Berkeley MacroLab,NA "DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate)",Biolegend,Cat#422801 Recombinant human IL2 protein,CETUS,NA Mouse IL-15 Recombinant Protein,PeproTech,Cat# 210-15-100UG Recombinant

(18) MECOM promotes leukemia progression and inhibits mast cell differentiation through functional competition with GATA2LeukemiaMay 5, 2026Kohei Iida, Mayuko Nakanishi, Jakushin Nakahara, Shuhei Asada, Tomoya Isobe, Tomohiro Yabushita, Tsuyoshi Fukushima, Yosuke Tanaka, Manabu Ozawa, Yasuhiro Yamada, et al.(Thermo Fisher, A11030) as the secondary antibody. Cell nuclei were stained with DAPI (BioLegend, catalog 422801). Fluorescence images were analyzed on an EVOS imaging system (Invitrogen). Luciferase assay 293 T cells

(19) Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infectionNature immunologyMay 1, 2026Liu W, Ping J, Deng L, Wang K, Xu J, Peng N, Yang M, Tang H, Liu Z, Wang W, et al.containing annexin V (5 ng ml−1) and either propidium iodide (100 ng ml−1, P4170, Sigma) or DAPI (40 nM, 422801, BioLegend). The samples were then analyzed using either a LSR Fortessa or a Symphony A5 flow cytometer

(20) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.Millipore Sigma, # P4170), 7-AAD (1 μg ml–1, Millipore Sigma, #A9400) or DAPI (0.5 μg ml–1, BioLegend, #422801) was added as a nuclear counterstain. For live cell staining without cell permeabilization, after cell

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(1)

3/28/2023Great DAPI for Immunocytochemistry Umut TankI use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really low. So, it will last forever.
(1)

7/12/2022DAPI Nuclear Stain Matthew RatsepI use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.

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DAPI from BioLegend

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