DAPI from BioLegend

Product Specs
ItemDAPI
CompanyBioLegend
Price $101.00Supplier Page View Company Product Page
Catalog Number422801
Quantity10 mg
ApplicationsICFC, ICC
FormatLyophilized
TargetDAPI

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Simultaneous gene engineering of T cells with multiple neoantigen-specific T cell receptors for adoptive cell transferMol Ther AdvSeptember 10, 2026Melinda Magna, Lior Levy, Krisztian Csomos, Aaron J. Dinerman, Abraham A. Hakim, Noam Levin, Sanghyun P. Kim, Nikolaos Zacharakis, Neilesh B. Parikh, Brian D. Bui, et al.anti-mouse TCR-β FITC (BD #553171) propidium iodide (#P1304MP, Thermo Fisher), or DAPI (BioLegend, #422801). Phenotyping panel: live/dead stain (eBioscience fixable viability dye eFluor 780, #65-0865-14), CD3-BUV395

(2) Rapid discovery of monoclonal antibodies via high-throughput single BCR affinity sequencingNucleic Acids ResAugust 20, 2026Mengzhu Hu, Qiuyu Lian, Xiaonan Cui, Xiao Chen, Xue Dong, Mengge Huang, Guangchao Wang, Yan Hu, Hao Zhang, Jiacan Su, et al.were washed twice after 30 min of incubation on ice. DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate, 422801, Biolegend) was used to label dead cells. DAPI−/B220 PE+/antigen AF647+ cells were sorted with a CytoFLEX

(3) Propionic Acid Remodels Mitochondrial Metabolism in SH-SY5Y CellsBiology (Basel)August 18, 2026Caitlyn Mahony, Erin Buchanan, Colleen O'Ryan90 min at 4 °C. Lastly, samples were counterstained with 250 nM Dapi (BioLegend, San Diego, CA, USA, 422801) for 10 min, washed and mounted on 26 × 76 mm × 1.2 mm glass microscope slides using 7 μL of Antifade

(4) P4HA3-mediated hydroxylation of SLC7A11 drives tyrosine kinase inhibitor resistance by suppressing ferroptosis in clear cell renal cell carcinomaRedox BiolAugust 14, 2026Yueming Chen, Guixin Zhang, Ruixin Yang, Yifan Li, Haozhuo Song, Zeping Chen, Hao Cai, Qingquan Li, Chen Fang, Jingyi Huang, et al.antibodies for 1 h at room temperature in the dark. Nuclei were counterstained with DAPI (Biolegend, #422801). Fluorescence images were acquired using a confocal laser scanning microscope. 2.18 Gene expression

(5) Genome-wide CRISPR screening identifies cellular factors controlling nonviral genome editing efficiencyNat CommunAugust 13, 2026Shivani Saxena, Meha Kabra, Amr A. Abdeen, Diana M. Tabima, Divya Sinha, Piper A. Rawding, Min Zhu, Ruosen Xie, Tanvi Kulkarni, Giovanni M. Hanstad, et al.488–conjugated donkey anti-mouse (Invitrogen, A21202, 1:500) secondary antibodies were used. DAPI (Biolegend, 422801, 1:1000) labeled the nucleus. A confocal microscope (Nikon C2 Instruments) was used to capture z-stack

(6) Lysosomal dysfunction drives a transcriptional and epigenetic signature characteristic of disease-associated microglia in neurodegenerative diseasesImmunityAugust 11, 2026Christopher D. Balak, Johannes C.M. Schlachetzki, Addison J. Lana, Elizabeth West, Christine Hong, Jordan DuGal, Yi Zhou, Benjamin Li, Nathanael J. Spann, Payam Saisan, et al.centrifuged at 800xg for 5 minutes at 4°C in a swinging bucket centrifuge. DAPI (1:1,000; BioLegend 422801) was used to label nuclei. Samples were strained through a 70 μm mesh strainer, washed once more, resuspended

(7) Temporal changes in metabolism guide oligodendrocyte precursor cell dynamics in aging and multiple sclerosisNeuronAugust 7, 2026Tess Dierckx, Sarah E. Wilson, Rebecca Buchanan, Themistoklis M. Tsarouchas, Yuhsiang Cheng, Maria Sacconi Nunez, Kelly Strickland, Dena Kumsa, Yohan S. S. Auguste, Sam Vanherle, et al.Afterwards, the cells were detached with 0.05% Trypsin-EDTA (Thermofisher, 25300054), DAPI (Biolegend, 422801) was added to determine cell viability, and MitoSox MFI was assessed in the DAPI-MitoSox+ cell population

(8) Protocol for generation of a 3D humanized lung-tumor-on-a-chip model of immune-hot and immune-cold microenvironmentsSTAR ProtocJuly 23, 2026Sabrina Brito Añez, Sanne van Kesteren, Peter Carmeliet, Anna Rita Cantelmo, Patricia Yagüe, Jeffrey Kroon,Cell Signaling Technology,8953 Mouse anti-human CD31 Alexa Fluor 647 1:200,BD Pharmingen,561654 DAPI 1:5000,BioLegend,422801 Hoechst 1:500,Thermo Fischer Scientific,62249 ,, Biological samples,Biological samples,Biological

(9) Emergency myelopoiesis in murine arthritis results in cell-intrinsic changes in dendritic cell development and functioniScienceJuly 17, 2026Lin Tze Tung, Roselyn R. Jiang, Bianca Pozzebon, Mathieu Mancini, Lucie Droin, Mitra Yousefi, Joo Eun (June) Kim, Marwah Mousa, Yue Liang, Connie M. Krawczyk, et al.Scientific,SB-4401-75 Viability Dye eFluor 506,ThermoFisher Scientific,65-0866-14 DAPI,Biolegend,422801 Fc blocker TruStain FcX,Biolegend,101320 Proliferation Dye eFluor 450,ThermoFisher Scientific,65-0842-85 Lipopolysaccharide,Millipore-Sigma,"LPS25,

(10) miR-205-5p promotes endothelial dysfunction and senescence in pulmonary fibrosisJCI InsightJuly 8, 2026Giuseppe Muscato, Benjamin B. Roos, Sharonda Harris, Xiaoyu Tracy Cai, Gina Civettini, Enrico Sciacca, Ahmed A. Raslan, Alessandra Castaldi, Sharon Elliot, Marilyn K. Glassberg, et al.(Thermo Fisher Scientific, 1:500 dilution, RRID:AB_2534069) and counterstained with DAPI (Biolegend, 422801, 1:1,000 dilution), which was added for 2 hours at room temperature. All antibodies were commercially

(11) Eliminating PD‐L1 on Dendritic Cell Extracellular Vesicles for Immunotherapy Potentiates Immune‐Mediated Tumour Rejection in MiceJournal of Extracellular VesiclesJuly 1, 2026Loes Teeuwen, Loïc Steiner, Chantal Reinhardt, Annemarijn Offens, Jesse E. Kuipers, Daniel Martínez‐Martínez, Jules Mazouin, Benedict J. Chambers, Gözde Güçlüler Akpinar, Susanne Gabrielssonco‐cultures were resuspended in 200 µL AnnexinV binding buffer or PBS, respectively, containing 3 µM DAPI (422801, BioLegend). Isolated cells from spleens, tumours and TDLNs were stained and incubated for 30 min at

(12) TROP2 targeting reveals therapy-driven cell state dynamics in colorectal cancerNatureJuly 1, 2026Nuria Vaquero-Siguero, Nikolaos Georgakopoulos, Maria C. Puschhof, Ioannis Chiotakakos, Jasmin Meier, Sigrid K. Fey, Gabriele Diamante, Manuel Mastel, Aitana Guiseris-Martinez, Guillaume Belthier, et al.FACS buffer (PBS with 1% fetal calf serum) and resuspended in 200-µl FACS buffer with DAPI (BioLegend; 422801) or Zombie NIR (BioLegend; 423105), which were used as death exclusion markers. The samples were analysed

(13) Bispecific antibody engineered extracellular vesicles redirect T cells to prevent postoperative epidural fibrosisNature CommunicationsJune 27, 2026Shanwei Ye, Qian Xu, Yong Xu, Hui Lu, Zhenfeng Liu, Jianfeng Guo, Yichuan Li, Wei Wang, Jun Ran, Xiaohua Zhu, et al.positivity. Following staining, cells were resuspended in FACS buffer containing DAPI (Biolegend, catalog 422801) prior to flow cytometry analysis. Luciferase assays Primary mouse T cells were purified from spleens

(14) CD177 Deficiency Defines a Stable Subtype of Human Neutrophil Granulocytes with Tumor Promoting ActivityAdvanced ScienceJune 22, 2026Marcel Jung, Alexander Beer, Susmita Ghosh, Ekaterina Pylaeva, Belal Alshaar, Tobias Tertel, Nils Bastian Leimkühler, Thomas Schroeder, Janine Gronewold, Nina Hagemann, et al.was carried out at RT for 1 h, followed by washing with PBS. DAPI (1:200 in PBS) (BioLegend; Cat. # 422801) staining was performed with an incubation time of 30 min. Fluorescence images were acquired with an

(15) Enhanced efficacy of gastric cancer treatment by a combination of NK cells and chemotherapyiScienceJune 19, 2026Jingjing Yue, Jie Ma, Yangyang Li, Hongbo Dong, Yongge He, Minhua Chen, Fang Fang, Weihua XiaoC-5138 DNASE I,Sigma,Cat# C-5025 "D-Luciferin,Potassium Salt",GOLDBIO,Cat# 115144-35-9 DAPI,Biolegend,Cat# 422801 Mouse serum,Future,Cat# F001008 "1,1′-dioctadecyl-3,3,3′,3′-tetramethy lindocarbocyanine perchlorate",Thermo

(16) An ITGA11 expressing subpopulation as predictor for the donor-specific osteogenic capacity of stromal cellsBone ResJune 15, 2026Ali Jasim Mohammad Jamil, Kaja Madsen, Souad Daamouch, Birgitte Villadsen, Chao Ma, Shakespeare Jeromdesella, Mikkel Ørnfeldt Nørgård, Emilie Grupe, Maria Bruun Jakobsson, Yuting Wang, et al.fluorophores except one, for FACS were used as negative controls. Dead cells were excluded using 1 μg/mL DAPI (422801, BioLegend). Due to a high number of utilized fluorophores, compensation was required to correct for

(17) Topical delivery of a PPARγ agonist enables adipogenic repair of irradiated skinMolecular Therapy AdvancesJune 11, 2026Ashley Siegel, Samerender Hanumantharao, Pratik Koirala, Viren Sehgal, Chaoqi He, Menfang Wu, Soheila Ali Akbari Ghavimi, Ziyu Chen, Zachary Williams, Xi Xu, et al.(Invitrogen #A32731, 1:500; AAT Bioquest #16837, 1:300), and counterstained with DAPI (BioLegend, #422801, 1:500). Images were acquired at 40× using an Olympus Fluoview confocal microscope with standardized

(18) Ultrastructural diversity and subcellular organization of nigral Lewy pathology in Parkinson’s diseaseNature CommunicationsJune 8, 2026Amanda J. Lewis, Lukas van den Heuvel, Marta Di Fabrizio, Kaliya Bandelier, Daria Proniakova, Domenic Burger, Notash Shafiei, Kazadi Ekundayo, Selene Offringa, Evelien Huisman, et al.Molecular Probes A32787 - 1/400, chicken 647 Molecular Probes A32933 - 1/400) and DAPI (Biolegend #422801 - 1/800 dilution) to label cell nuclei after incubation at room-temperature for 30 mins. 3D fluorescent

(19) CCL19⁺ fibroblasts define a proliferative niche in chronic lymphocytic leukemiabioRxivMay 30, 2026Antonio P. Ferreira, Shumei Wang, Larysa Poluben, Dominique Morais, Joshua Brandstadter, Eric Perkey, Steven Sotirakos, Mai Drew, Li Pan, Madeleine E. Lemieux, et al.min with Fc Blocking Reagent (Biolegend, cat. #422302) followed by DAPI (Biolegend, 1 μg/mL, cat. #422801) in FACS buffer (phosphate buffered saline [PBS] containing 2% fetal bovine serum). Cells were subsequently

(20) Limited Immune-Mediated Efficacy of Anti-PD-L1/VEGF in EGFR-TKI-Naïve Egfr-Mutant Lung Cancer with Non-Inflamed Tumor MicroenvironmentCurrent OncologyMay 27, 2026Atsuko Hirabae, Tadahiro Kuribayashi, Shuta Tomida, Sachi Okawa, Takamasa Nakasuka, Kazuya Nishii, Jun Nishimura, Go Makimoto, Kiichiro Ninomiya, Hisao Higo, et al.(FITC, GK1.5, or PE/Cy7, GK1.5) and CD8α (APC/Cy7, 53-6.7) from BioLegend (San Diego, CA, USA). DAPI (#422801) or the Zombie NIR Fixable Viability Kit (#423105) purchased from BioLegend were used for dead cell

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(1)

3/28/2023Great DAPI for Immunocytochemistry Umut TankI use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really low. So, it will last forever.
(1)

7/12/2022DAPI Nuclear Stain Matthew RatsepI use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.

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DAPI from BioLegend

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