Cyto-Last™ Buffer from BioLegend

Product Specs
ItemCyto-Last™ Buffer
CompanyBioLegend
Price $77.00Supplier Page View Company Product Page
Catalog Number422501
Quantity100 mL
ApplicationsICFC - Quality tested

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou Jiang(10x),BioLegend,Cat# 421002 Fixation Buffer,BioLegend,Cat# 420801 Cyto-Last™ Buffer,BioLegend,Cat# 422501 Cell Staining Buffer,BioLegend,Cat# 420201 Opal Polaris 7-Color Manual IHC Kit,AKOYA Biosciences,Cat#

(2) Daytime SHP2 inhibitor dosing, when immune cell numbers are elevated, shrinks neurofibromasLife Science AllianceDecember 1, 2025Niousha Ahmari, Kwangmin Choi, Jianqiang Wu, Tilat A Rizvi, Mark Jackson, Leah J Kershner, Mi-Ok Kim, Xiyuan Zhang, Eva Dombi, Jack Shern, et al.and resuspended in 350 μl in PBS for immediate analysis, or stored in 500 μl Cyto-Last Buffer (Cat #422501; BioLegend), and then, cells were analyzed on a Cytek Aurora flow cytometer with a 5-laser configuration

(3) Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosisCell ReportsMarch 25, 2025Samantha M. Golomb, Ian H. Guldner, Emilija Aleksandrovic, Shaneann R. Fross, Xiyu Liu, Lu Diao, Karena Liang, Jinxuan Wu, Qingfei Wang, Jacqueline A. Lopez, et al.True-Nuclear Transcription Factor Buffer Set (BioLegend, 424401) and resuspended in CytoLast (BioLegend, 422501) for overnight storage in the dark at 4C. The following morning, cells were permeabilized using the

(4) Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosisNature communicationsNovember 16, 2023Parveen S, Shen J, Lun S, Zhao L, Alt J, Koleske B, Leone RD, Rais R, Powell JD, Murphy JR, et al.BioLegend) following the manufacturer’s protocol. The stained cells were stored in Cyto-Last™ Buffer (Cat: 422501; BioLegend) at 4 °C till the data acquisition. The data were acquired on BD LSRFortessa™ Cell Analyzer

(5) Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse modelsSTAR protocolsMarch 17, 2023Chen A, Chao Y, Zou H, McCarl LH, Kohanbash G, Hu B.True-Nuclear™ Transcription Factor Buffer Set,BioLegend,Cat #424401 Fixation buffer,BioLegend,Cat #420801 Cyto-Last™ Buffer,BioLegend,Cat #422501 "Paraformaldehyde, 32% w/v aq. soln., methanol free",Fisher Scientific,Cat# AA47377-9M Ketamine,Covetrus,Cat#

(6) T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancerOncoimmunologyJanuary 1, 2021O'Connor RA, Chauhan V, Mathieson L, Titmarsh H, Koppensteiner L, Young I, Tagliavini G, Dorward DA, Prost S, Dhaliwal K, et al.deluxe Human IL-6 ELISA,Biolegend,Biolegend Cat # 430504 Cyto-last buffer,Biolegend,Biolegend Cat # 422501 One comp eBeads,Thermo Fisher,Thermor Fisher Cat # 01–1111-42 Human IL-27 DuoSet ELISA,RnD systems,RnD

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Cyto-Last™ Buffer from BioLegend

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Cyto-Last™ Buffer from BioLegend

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