Fig 1: Reduction of Npnt mRNA expression by OSM. MC3T3-E1 cells were treated with 100 ng/ml of OSM, LIF, IL-6, or IL-11 for 24 h. Total cellular RNA was extracted, and mRNA levels for Npnt and Gapdh were examined by real-time PCR analysis. Results are shown as the mean ± SD from 3 samples as compared to the level at 0 ng/ml (without cytokine treatment). ∗∗P < 0.01, ∗P < 0.05, Student’s t test relative to the level without cytokine treatment.