M-MLV Reverse Transcriptase, RNase H Minus M5301 from Promega

Product Specs
ItemM-MLV Reverse Transcriptase, RNase H Minus
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM5301
Size10,000u

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Atg2 coordinates microbial metabolite signaling and epigenetic remodeling to maintain intestinal lipid homeostasis in DrosophilaMicrobiomeMarch 27, 2026Ping Wang, Xinran Li, Jiangong Zhang, Jiewei Wang, Li Hua Jinextracted using TRIzol Reagent. The RNA was reverse-transcribed via M-MLV reverse transcriptase (Promega M5301). SYBR GreenER (Invitrogen 11,762,500) and rp49 normalization were used for qPCR (primers in Table S2

(2) Loss of meningeal lymphatic endothelial cells results in formation of a newly forming vascular network with scavenging functionDevelopmentFebruary 15, 2026Katharina Uphoff, Andreas van Impel, Lars Muhl, Martin Stehling, Christer Betsholtz, Stefan Schulte-Merkerwere performed as described in the manufacturer's instructions [M-MLV RT (H–), Point Mutant (Promega, M5301); SYBR™ Green Universal Master Mix (Applied Biosystems, 4309155)] and normalized against the expression

(3) An Alternative DNA Endonuclease Activity is Associated with the LINE-1 ORF2-encoded ProteinbioRxivJanuary 23, 2026Mitsuhiro Nakamura, Huira C. Kopera, Mark Dowling, Orsolya Barabas, John V. Moransimilar results. Complementation reverse transcription-PCR (RT-PCR) assay MMLV RT (RNase H-) (Promega M5301) and Induro RT (NEB M0681S) were diluted to 10 U/μL in RT Buffer (50 mM Tris-HCl pH 7.5, 50 mM KCl,

(4) NEAT and tidy: a novel RT-PCR method for the independent quantification of the NEAT1_1 isoformBiology Methods and ProtocolsOctober 11, 2025Nadine E Smith, Janni Petersen, Michael Z MichaelmM,Promega,U1511,"Waltham, MA, USA" 100 U/µl M-MLV RNase H minus reverse transcriptase,4 U/µl,Promega,M5301,"Madison, WI, USA" Master mix details for cDNA generation.

(5) 9-cis-Retinoic Acid Improves Disease Modelling in iPSC-Derived Liver OrganoidsCellsJune 26, 2025Mina Kazemzadeh Dastjerd, Vincent Merens, Ayla Smout, Rebeca De Wolf, Christophe Chesné, Catherine Verfaillie, Stefaan Verhulst, Leo A. van GrunsvenReliaPrep RNA Cell Miniprep System,Promega,Cat# Z6012 "M-MLV Reverse Transcriptase, RNase H Minus and Point Mutant",Promega,Cat# M5301 GoTaq qPCR Master Mix with BRYT Green Dye,Promega,Cat# A6002 P450-Glo CYP3A4 activity assay,Promega,Cat#

(6) Putative G‑Quadruplex Structures in Dysregulated Long Non-coding RNA of Ovarian Cancer and Their Binding Interactions with Human Serum AlbuminACS OmegaJune 24, 2025Deepshikha Singh, Chinmayee Shukla, Bhaskar Dattafollowed by cooling to room temperature. Four U/μL per reaction reverse transcriptase (Catalog no. M5301, Promega Biotech India Pvt. Ltd., New Delhi) was added with a buffer of 3 mM MgCl2, 10 mM Tris-Cl pH

(7) Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seqNat CommunFebruary 10, 2025Cheng Ding, Guoting Chen, Shiping Luan, Runxin Gao, Yudong Fan, Ying Zhang, Xiaoting Wang, Guoliang Li, Mohamed F. Foda, Jiapei Yan, et al.transcription solution (1× MMLV buffer, 0.5 mM dNTP, 8U/μl MMLV (RNase H-), 1U/μl RiboLock RNase) (Promega; M5301), and then incubated at 42 °C for 1 h. The isolated nuclei were washed three times with 500 μl 1× wash

(8) Rspo3-mediated metabolic liver zonation regulates systemic glucose metabolism and body mass in micePLoS biologyJanuary 1, 2025Uno K, Uchino T, Suzuki T, Sayama Y, Edo N, Uno-Eder K, Morita K, Ishikawa T, Koizumi M, Honda H, et al.(#N2111, Promega Corporation, Madison, Wisconsin, USA) and M-MLV-Reverse-Transcriptase-RNase-H-Minus (#M5301, Promega Corporation). Quantitative RT-PCR was performed by Taqman analysis (Thermo Fisher Scientific,

(9) Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expressionCell ReportsMarch 1, 2024Marisa Connell, Yonggang Xie, Xiaobing Deng, Rui Chen, Sijun ZhuK,Sigma Aldrich,#124568 DNase I,New England Biolabs,#M0303 MMLV H- Reverse transcriptase,Promega,#M5301 DreamTaq Hot Start Green PCR Master Mix,ThermoFisher Scientific,#K9021 EcoRI,ThermoFisher Scientific,#ER0271 XhoI,ThermoFisher Scientific

(10) Aquaporin 1 overexpression may enhance glioma tumorigenesis by interacting with the transcriptional regulation networks of Foxo4, Maz, and E2F familiesChin Neurosurg JDecember 6, 2023Ying Guan, Jinhua Han, Die Chen, Yuefu Zhan, Jianqiang Chenagarose gels. RNA was reverse-transcribed into cDNA using the M-MLV Reverse Transcriptase (Promega, Lot# M5301). RNA sequencing and analysis The library construction and Illumina sequencing were performed at WuXi

(11) A conserved long-distance telomeric silencing mechanism suppresses mTOR signaling in aging human fibroblastsScience advancesAugust 19, 2022Jäger K, Mensch J, Grimmig ME, Neuner B, Gorzelniak K, Türkmen S, Demuth I, Hartmann A, Hartmann C, Wittig F, et al.following the manufacturer’s instructions [M-MLV Reverse Transcriptase, RNase (H−), Promega, catalog no. M5301]. One microgram of mRNA was used for the reverse transcriptase reaction. qPCR was carried out using

(12) In vivo discovery of RNA proximal proteins via proximity-dependent biotinylationRNA biologyDecember 1, 2021Lin X, Fonseca MAS, Breunig JJ, Corona RI, Lawrenson K.Scientific, Catalogue number: 10,296,028). RT-qPCR M-MLV reverse transcriptase (Promega, Catalogue number: M5301) and random hexamers (Promega, Catalogue number: C1181) were used for reverse transcription of RNA extracted

(13) Single-cell transcriptomics identifies gene expression networks driving differentiation and tumorigenesis in the human fallopian tubeCell reportsApril 13, 2021Dinh HQ, Lin X, Abbasi F, Nameki R, Haro M, Olingy CE, Chang H, Hernandez L, Gayther SA, Wright KN, et al.,Thermo Fisher Scientific,31–985-070 Lipofectamine RNAiMAX,Thermo Fisher Scientific,13–778-150 M-MLV reverse transcriptase RNase H,Promega,M5301 Random primers,Promega,C1181 Taqman Universal Master Mix with UNG,Thermo Fisher Scientific,4440044 HPRT1

(14) Enhanced Autolysosomal Function Ameliorates the Inflammatory Response Mediated by the NLRP3 Inflammasome in Alzheimer’s DiseaseFrontiers in Aging NeuroscienceFebruary 23, 2021Wen Zhou, Deng Xiao, Yueyang Zhao, Botao Tan, Zhimin Long, Lehua Yu, Guiqiong Heto extract total RNA from each group of cells. Random hexamer primers and MMLV reverse transcriptase (m5301, Promega, USA) were used to reverse transcribe cDNA. SGExcel FastSYBR Mixture (B532954, Sangon Biotech,

(15) Dynamic changes of autophagic flux induced by Abeta in the brain of postmortem Alzheimer's disease patients, animal models and cell modelsAgingJune 13, 2020Long Z, Chen J, Zhao Y, Zhou W, Yao Q, Wang Y, He G.Reverse transcription was then performed using random hexamer primer and MMLV Reverse Transcriptase (M5301, Promega). Quantitative PCR (qPCR) for the quantitation of gene transcripts was performed with 2×HotStart

(16) miR-15b reduces amyloid-β accumulation in SH-SY5Y cell line through targetting NF-κB signaling and BACE1Bioscience ReportsDecember 21, 2018Juan Li, Haitao Wanginstructions. Reverse transcription was then performed using random hexamer primer and MMLV Reverse Transcriptase (M5301; Promega). Quantitative PCR (qPCR) for the quantitation of gene transcripts was performed with 2× HotStart

(17) Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper proteinNucleic Acids ResearchNovember 16, 2018C Joaquín Cáceres et al.5′fluorescently labeled (D2 or D4 WellRED, Sigma Aldrich) and M-MLV RNAse H− reverse transcriptase (#M5301; Promega). RNAs were denatured for 3 min at 95°C with 1 μl of DMSO and cooled in ice for 3 min. Three

(18) Expression of tissue factor in human cervical carcinoma tissueExperimental and Therapeutic MedicineSeptember 11, 2018Xitong Zhao, Chu Cheng, Jinhai Gou, Tao Yi, Yanping Qian, Xue Du, Xia Zhaokit (RR066A; both Takara Bio, Inc., Otsu, Japan), the M-MLV Reverse Transcriptase RNase H Minus kit (M5301; Promega Corporation, Madison, WI, USA) and the BCA protein concentration determination kit (P0009;

(19) A tricyclic antidepressant, amoxapine, reduces amyloid-β generation through multiple serotonin receptor 6-mediated targetsScientific ReportsDecember 1, 2017Xiaohang Li, Qinying Wang, Tingting Hu, Ying Wang, Jian Zhao, Jing Lu, Gang Peiaccording to the manufacturer’s instructions. Random hexamer primers and MMLV Reverse Transcriptase (M5301; Promega) were used for reverse transcription. All gene transcripts were quantified by quantitative real-time PCR

(20) Estrogen stimulates osteoprotegerin expression via the suppression of miR-145 expression in MG-63 cellsMolecular medicine reportsApril 1, 2017Jia J, Zhou H, Zeng X, Feng S.added to the M-MLV 5X Reaction Buffer, dNTPs, RNasin (Promega Corporation), M-MLV RT enzyme (cat. no. M5301; Promega Corporation), and nuclease-free water to final volume of 25 µl, then was incubated for 1 h

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M-MLV Reverse Transcriptase, RNase H Minus

M-MLV Reverse Transcriptase, RNase H Minus from Promega

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M-MLV Reverse Transcriptase, RNase H Minus from Promega

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