Protease Inhibitor Cocktail, 50X G6521 from Promega

Product Specs
ItemProtease Inhibitor Cocktail, 50X
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6521
Size1ml
Molecular Weight~114kDa
Applicationspurification
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Prickle and Ror modulate Dishevelled-Vangl interaction to regulate non-canonical Wnt signaling during convergent extension in XenopuseLifeApril 30, 2026Hwa-seon Seo, Deli Yu, Ivan K Popov, Jiahui Tao, Allyson R Angermeier, Fei Yang, Sylvie Marchetto, Jean-Paul Borg, Bingdong Sha, Jeffrey D Axelrod, et al.7.5), 150 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% Triton-X100, and 1 x protease inhibitor (Promega #G6521). Western blot detection of proteins was carried out with anti-GFP antibody (Santa Cruz Biotechnology

(2) Technical and biological sources of noise confound multiplexed enhancer AAV screeningNat CommunApril 28, 2026Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, et al.Homogenization Buffer containing 0.1 mM DTT (Promega P1171), 1x Protease Inhibitor Cocktail (Promega G6521), 0.2 U/µL RNasin Plus RNase Inhibitor (Promega N2615), 0.1% Triton X-100 dissolved in 0.22 μm filtered

(3) Integrative Structural Modeling of Intrinsically Disordered Regions in a Human HDAC2 Chromatin Remodeling ComplexbioRxivMarch 27, 2026Jules Nde, Cassandra G. Kempf, Rosalyn C. Zimmermann, Joseph Cesare, Ying Zhang, Jerry L. Workman, Laurence Florens, Michael P. WashburnMammalian Lysis Buffer (G938A), FuGENE® HD Transfection Reagent (E2311), and Protease Inhibitor Cocktail (G6521) were purchased from Promega. Clone FHC02563 containing the HDAC1 open reading frame was purchased from

(4) Profiling the pancreatic cancer secretome with metabolic glycoengineeringJournal of Biological ChemistryMarch 1, 2026Kris Dammen-Brower, Stanley Zhu, Christian Agatemor, Safiya Aafreen, Vrinda Dharharma, Christopher T. Saeui, Hui Li, Jian Song, Matthew J. Buettner, Keith R. Kwagala, et al.extracted from cell lysate following resuspension in a 1:50 ratio of protease inhibitor cocktail (Promega, G6521) in radioimmunoprecipitation assay (RIPA) lysis buffer (MilliporeSigma, R0278-50ML) and placed in −20

(5) Differential Effects of Apigenin on Normal and Squamous Oral Epithelial Cells Reveal Redox–Autophagy Signaling Vulnerabilities in OSCCInternational Journal of Molecular SciencesFebruary 24, 2026Bianca Voicu Balasea, Miruna-Silvia Stan, Miruna Dinescu, Marina Imre, Radu Radulescu, Ana Cernega, Monica Musteanu, Alexandra Ripszky, Silviu Mirel PituruSignaling Lysis Buffer supplemented with Protease Inhibitor Cocktail (Promega, Madison, WI, USA; Cat. No. G6521). The filtered lysates were stored at −80 °C in 1.5 mL Eppendorf tubes for subsequent analyses. Total Protein Kit, Micro

(6) Cholinergic signaling modulates intestinal pathophysiology in a Drosophila model of cystic fibrosisPLOS GeneticsFebruary 17, 2026Elizabeth A. Lane, Afroditi Petsakou, Ying Liu, Weihang Chen, Mujeeb Qadiri, Yanhui Hu, Norbert Perrimon25mM KCl, 5mM MgCl, 0.1% Triton-X, 0.5% RNasin Plus (Promega, N2615), 50x protease inhibitor (Promega G6521), 0.1mM DTT]. Using 1ml dounce (Wheaton 357538), nuclei were released by 20 loose pestle strokes and

(7) Structural Mechanisms of SAMD9 Autoinhibition and Pathogenic DysregulationbioRxivFebruary 3, 2026Zongjun Mou, Fushun Zhang, Marisol Morales, Bibekananda Sahoo, Xinghong Dai, Yan Xiangmammalian lysis buffer (Promega G9381) supplemented with 200 μl Protease Inhibitor Cocktail (Promega G6521), 8 μl Benzonase (Millipore 70664–3), and 50 μl of 1 M MgCl2. Lysates were diluted 1:3 with HaloTag

(8) The RNA exosome maintains cellular RNA homeostasis by controlling transcript abundance in the brainCell ReportsJanuary 27, 2026Lauryn A. Higginson, Xingjun Wang, Kevin He, Maggie Torstrick, Minhoo Kim, H.R. Sagara Wijeratne, Avery M. Runnebohm, Bérénice A. Benayoun, Adam MacLean, Guillaume F. Chanfreau, et al.T9284-100ML RNasin® Plus Ribonuclease Inhibitor,Promega,Cat# N2611 "Protease Inhibitor Cocktail, 50X",Promega,Cat#G6521 DL-Dithiothreitol solution,Millipore Sigma,Cat# 43816-10ML "Albumin, Bovine Serum, Fraction V, Fatty

(9) ASPPs multimerize protein phosphatase 1PLoS GenetOctober 16, 2025Derek T. Wei, Kayleigh N. Morrison, Gwendolyn M. Beacham, Erika Beyrent, Cyrus A. Habas, Ying Zhang, Laurence Florens, Gunther Hollopeterthawed in 300 μL Mammalian Lysis Buffer (PAG9381, Promega) with 6 μL 50x Protease Inhibitor Cocktail (G6521, Promega) and sheared using a 25 gauge needle. JFX549 (gifted from Luke Lavis, Janelia Research Campus)

(10) Dysregulated Protein s -Nitrosylation Promotes Nitrosative Stress and Disease Progression in Heart Failure With Preserved Ejection FractionCirculation ResearchOctober 10, 2025Zhen Li, Kyle B. LaPenna, Natalie D. Gehred, Xiaoman Yu, W.H. Wilson Tang, Jake E. Doiron, Huijing Xia, Jingshu Chen, Ian H. Driver, Frank B. Sachse, et al.7.5, 25 mmol/L KCl, 5 mmol/L MgCl2, 45 μmol/L actinomycin D, supplemented with 1X protease inhibitor (G6521, Promega), 0.4 U/μL RNasin ribonuclease inhibitor (N2515, Promega), 0.2 U/μL SuperaseIn (AM2694, Thermo

(11) Low Dose GLP-1 Therapy Attenuates Pathological Cardiac and Hepatic Remodelling in HFpEF Independent of Weight LossbioRxivOctober 2, 2025Mahmoud H. Elbatreek, Zhen Li, Xiaoman Yu, Natalie D. Gehred, Tatiana Gromova, Jingshu Chen, Naoto Muraoka, Martin Jensen, Vinay Kartha, Chris Carrico, et al.Tris-Hcl pH 7.5, 25mM KCl, 5mM MgCl2, 45uM Actinomycin D, supplemented with 1X protease inhibitor (G6521, Promega), 0.4U/uL RNasin Ribonuclease Inhibitor (N2515, Promega), 0.2U/ul SuperaseIn (AM2694, ThermoFisher).

(12) Hydrogen Sulfide Deficiency and Therapeutic Targeting in Cardiometabolic HFpEF: Evidence for Synergistic Benefit With GLP-1/Glucagon AgonismJACC. Basic to translational scienceOctober 1, 2025Doiron JE, Elbatreek MH, Xia H, Yu X, Gehred ND, Gromova T, Chen J, Driver IH, Muraoka N, Jensen M, et al.7.5, 25 mmol/L KCl, 5 mmol/L MgCl2, 45 μmol/L Actinomycin D, supplemented with 1× protease inhibitor (G6521, Promega), 0.4 U/uL RNasin Ribonuclease Inhibitor (N2515, Promega), 0.2 U/ul SuperaseIn (AM2694, ThermoFisher).

(13) A comprehensive method of isolating proteins from serum, cerebrospinal fluid, and hippocampal neurons in rats for proteomic profiling using the LC-MS/MS platformBiochemistry and Biophysics ReportsSeptember 1, 2025Pratibha Sharma, Rajinder K. Dhamijapre-sterilized stainless forceps, scissor, and razor (Z225622, Merck, Germany), PMSF Inhibitor Cocktail (G6521, Promega, United States), Trypsin gold, MS grade (Promega, United States), Corning tubes (15 mL, CLS430052,

(14) NAC couples protein synthesis with nascent polypeptide myristoylation on the ribosomeThe EMBO JournalAugust 26, 2025Sara Zdancewicz, Emir Maldosevic, Kinga Malezyna, Ahmad JomaapH 7.4, 100 mM KCl, 5 mM MgCl2, 0.5% IGEPAL, 1 mM DTT, 0.5× protease inhibitor cocktail (Promega REF G6521), 4 units/mL RNase inhibitor (ThermoFisher REF EO0381), and 100 µg/mL cycloheximide). The lysate was

(15) Interleukin-10 augments human endogenous retroviral E1B variant of cd5 in aged T cellsBlood ResAugust 11, 2025Bharat Singh, Smita Kumari, Amit Kumar Kureel, Arunim Shah, Shobhita Katiyar, Chandra Prakash Chaturvedi, Kulwant Singh, Ambak Kumar Raiagain solubilized in 300 µl RIPA buffer. RIPA buffer was added with 1 × protease inhibitor cocktail (G6521, Promega Biotech) and 10 mM phenylmethylsulfonyl fluoride before use. Chromatin shredding was performed

(16) Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naïve chromosomal locusNucleusAugust 1, 2025Sweta Sikder, Songjoon Baek, Yamini Dalal, Ganesan Arunkumarchromatin was extracted overnight using a low-salt buffer with 1× Protease Inhibitor Cocktail (catalog no. G6521, Promega, USA) at 4°C. The supernatant was incubated overnight at 4°C with appropriate antibodies. The

(17) Label-Free Proteomic Profiling of the dvls2 (CL2006) Caenorhabditis elegans Alzheimer's Disease (AD) Model Reveals Conserved Molecular Signatures Shared With the Human AD BrainJ NeurochemJuly 13, 2025Iverson Conrado Bezerra, Emily Raphaely Souza Dos Santos, Katarine G. Aurista do Nascimento, Artur José da Silva, Josivan Barbosa de Farias, Maria Luiza de Lima Vitorino, Roberto Afonso da Silva, José Luiz de Lima Filho, Priscila Gubertrecovered. During extraction, a protease inhibitor cocktail (Promega, Madison, Wisconsin, USA, cat. no. G6521) was added using the protein ratio of 1:50 w/w to the mixture. Following the extraction, the protein

(18) Cholinergic Signaling Modulates Intestinal Pathophysiology in a Drosophila Model of Cystic FibrosisbioRxivJuly 5, 2025Elizabeth Lane, Afroditi Petsakou, Ying Liu, Weihang Chen, Mujeeb Qadiri, Yanhui Hu, Norbert Perrimon25mM KCl, 5mM MgCl, 0.1% Triton-X, 0.5% RNasin Plus (Promega, N2615), 50x protease inhibitor (Promega G6521), 0.1mM DTT]. Using 1ml dounce (Wheaton 357538), nuclei were released by 20 loose pestle strokes and

(19) In vivo pulse-chase in Caenorhabditis elegans reveals intestinal histone turnover changes upon starvationJournal of Biological ChemistryJuly 1, 2025Christopher Borchers, Kara Osburn, Hyun Cheol Roh, Scott T. AokiHepes pH 7.5, 10 mM KCl, 1.5 mM MgCl2, and 0.1% Triton X-100) containing protease inhibitors (Promega, #G6521). Samples were cross-linked in 1.1% paraformaldehyde (Thermo Fisher Scientific, #50-980-487) for 4 min

(20) ASCL1 protein domains with distinct functions in neuronal differentiation and subtype specificationDevelopmental BiologyJuly 1, 2025Yuji Nakada, Madison J. Martinez, Jane E. JohnsonMgCl2, 100 mM KCl, 1 mM DTT, 0.2 % NP-40, 1 mM PMSF, and protease inhibitor cocktail (Promega Cat #G6521). After centrifugation at 20,000×g for 30 min, the supernatant was incubated with guinea pig anti-ASCL1

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Protease Inhibitor Cocktail, 50X

Protease Inhibitor Cocktail, 50X from Promega

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Protease Inhibitor Cocktail, 50X from Promega

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