ProTEV Plus V6102 from Promega

Product Specs
ItemProTEV Plus
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6102
Size8,000u
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Direct Optical Quantification of Chain Collapse, Reduced Dielectric, and Water Release Driving Protein Phase SeparationbioRxivNovember 10, 2025Ethan A. Perets, Jacob A. Spies, Justin H. Cheong, Lixue Shi, DeeAnn K. Asamoto, Alex S. Holehouse, Judy E. Kim, Wei Min, Jens Neu, Elsa C. Y. Yanwith 5 mL lysis buffer per 1 mL beads. TEV protease solution (20 uL ProTEV Plus, Promega, Cat. No. V6101, in 980 μL lysis buffer) was applied to 1 mL of GST-TEV-DDX4[1-245]-bound beads. Beads were incubated

(2) Soluble CSF1R promotes microglial activation and amyloid clearance in alzheimer's diseaseJ NeuroinflammationOctober 28, 2025Lianshuai Zhang, Kai Chen, Ruicheng Zeng, Guojun Bu, Xiao-Fen ChenResin (YEASEN, 36405ES25). The bound proteins were eluted and incubated with TEV protease (Promega, V6101), which contains an N-terminal His-tag, to remove the Fc domain. Following cleavage, the TEV protease

(3) Wheat tandem kinase RWT4 directly binds a fungal effector to activate defenseNature GeneticsMay 1, 2025Yi-Chang Sung, Yinghui Li, Zoe Bernasconi, Suji Baik, Soichiro Asuke, Beat Keller, Tzion Fahima, Gitta CoakerTris-HCl pH 8.0, 150 mM NaCl, 0.5 mM EDTA, 1 mM DTT) for TEV protease cleavage (ProTEV Plus, Promega, V6101). Samples were adjusted to a final volume of 10 ml with 100 U TEV protease and incubated at 4 °C overnight.

(4) SIRT7 Is a Lysine Deacylase with a Preference for Depropionylation and DemyristoylationInternational Journal of Molecular SciencesMarch 28, 2025Mohammad Golam Kibria, Tatsuya Yoshizawa, Tianli Zhang, Katsuhiko Ono, Tomoya Mizumoto, Yoshifumi Sato, Tomohiro Sawa, Kazuya YamagataPromega) overnight at 4 °C. The resin was washed with Halo purification buffer, and ProTEV Plus protease (#V6101; Promega) was employed to separate the recombinant sirtuin proteins from the Halo tag. 4.3. In Vitro

(5) Regulation of human interferon signaling by transposon exonizationCellDecember 26, 2024Giulia Irene Maria Pasquesi, Holly Allen, Atma Ivancevic, Arturo Barbachano-Guerrero, Olivia Joyner, Kejun Guo, David M. Simpson, Keala Gapin, Isabella Horton, Lily L. Nguyen, et al.protein tagged with the HaloTag and its protein partners, we used the ProTEV Plus enzyme (Promega; #V6101) to cleave the HaloTag from the bait protein following the protocol provided with the HaloTag Mammalian

(6) A rapid cell-free expression and screening platform for antibody discoveryNature communicationsJuly 3, 2023Hunt AC, Vögeli B, Hassan AO, Guerrero L, Kightlinger W, Yoesep DJ, Krüger A, DeWinter M, Diamond MS, Karim AS, et al.according to manufacturer instructions. His tags were removed via cleavage by ProTEV Plus (Promega, V6102). Before cleavage, 10% v/v glycerol was added to the protein. ProTEV Plus was added to a concentration

(7) FAM193A is a positive regulator of p53 activityCell reportsMarch 28, 2023Szwarc MM, Guarnieri AL, Joshi M, Duc HN, Laird MC, Pandey A, Khanal S, Dohm E, Bui AK, Sullivan KD, et al.Carfilzomib (PR-171),Selleck Chemicals,Cat#S2853 PreScission Protease,Cytiva,Cat#27-0843-01 ProTEV Plus,Promega,Cat#V6101 Thiazolyl Blue Tetrazolium Bromide,Sigma-Aldrich,Cat#M5655 cOmplete Mini,Roche,Cat#11836153001 Aprotinin,Gold Biotechnology

(8) TES-1/Tes and ZYX-1/Zyxin protect junctional actin networks under tension during epidermal morphogenesis in the C. elegans embryoCurrent BiologyDecember 1, 2022Allison M. Lynch, Yuyun Zhu, Bethany G. Lucas, Jonathan D. Winkelman, Keliya Bai, Sterling C.T. Martin, Samuel Block, Mark M. Slabodnick, Anjon Audhya, Bob Goldstein, et al.Alexa Fluor 488 Phalloidin,Invitrogen,Cat#A12379 Alexa Fluor 660 Phalloidin,Invitrogen,Cat#A22285 ProTEV Plus,Promega,Cat#V6102 polymerized chicken F-actin,"Cytoskeleton, Inc",Cat#AS99-B T7 Megascript kit,ThermoFisher,Cat#AM1334 T3

(9) Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exitBiology OpenNovember 1, 2022Marjan Abbasi, Alexander Julner, Yan Ting Lim, Tianyun Zhao, Radoslaw Mikolaj Sobota, Victoria MenÃ©ndez-Benitoperformed as previously described (24) and the SPBs were eluted with 5 μg proTEV plus protease (Promega, V6101) in TEV buffer overnight at 4°C. Trichloroacetic acid (TCA) was added to a 10% concentration, proteins

(10) Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signalingeLifeOctober 27, 2022Hwang WY, Kostiuk V, González DP, Lusk CP, Khokha MK.drug,Protease Inhibitor Cocktail mix,Millipore Sigma,P8340-5ML, Chemical compound and drug,ProTEV Plus,Promega,V6101, Chemical compound and drug,NEBExpress Ni-NTA Magnetic Beads,NEB,S1423S, Chemical compound and drug,Isopropyl

(11) Xist nucleates local protein gradients to propagate silencing across the X chromosomeCellDecember 9, 2021Markaki Y, Gan Chong J, Wang Y, Jacobson EC, Luong C, Tan SYX, Jachowicz JW, Strehle M, Maestrini D, Banerjee AK, et al.,Thermo Fisher Scientific,Cat #: EF0654 Protease Inhibitor Cocktail,Promega,Cat #: G6521 ProTEV Plus,Promega,Cat #: V6101 Vectashield,Vector Labs,Cat #: H-1000 DAPI,Thermo Fisher Scientific,Cat #: D1306 SpCas9 2NLS Nuclease

(12) UTX condensation underlies its tumour-suppressive activityNatureSeptember 1, 2021Shi B, Li W, Song Y, Wang Z, Ju R, Ulman A, Hu J, Palomba F, Zhao Y, Le JP, et al.fusion with MBP-tag. The MBP-tag was cleaved off the fusion proteins by ProTEV Plus (Promega, Cat# V6102). The second method was used for all other data in this paper. In this method, purified fluorescent

(13) The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst bindingCell reportsMay 25, 2021Kuramoto K, Kim YJ, Hong JH, He C.,Santa Cruz Biotechnology,Cat#sc-2003 IgG Sepharose 6 Fast Flow,Cytiva,Cat#17-0969-01 ProTEV Plus,Promega,Cat#V6101 SBI-0206965,ApexBio,Cat#A8715; CAS: 1884220-36-3 DNase I,Millipore Sigma,Cat#D5025; CAS: 9003-98-9 AICAR,Cayman Chemical

(14) Channel nuclear pore complex subunits are required for transposon silencing in DrosophilaeLifeApril 15, 2021Munafò M, Lawless VR, Passera A, MacMillan S, Bornelöv S, Haussmann IU, Soller M, Hannon GJ, Czech B.of 1X ProTEV Buffer, 1 mM DTT, and RNasin Plus (1:50) and 25 units of ProTEV Plus Protease (Promega V6101) and incubated 2 hr at 30°C, shaking at 1300 rpm. The supernatant containing the eluted protein and

(15) Cell-free reconstitution of multi-condensate assembliesMethods in EnzymologyJanuary 17, 2021Andrea Putnam, Geraldine Seydouxof the MBP fusion is driven by an IPTG-inducible promoter. TEV protease can be purchased (Promega #V6101) or purified as a 6× His-tag fusion (Addgene: pRK793). We recommend purifying TEV protease following

(16) Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesisNature cell biologyAugust 1, 2020Li W, Hu J, Shi B, Palomba F, Digman MA, Gratton E, Jiang H.phase separation assays, the MBP-tag was cleaved off the fusion proteins by ProTEV Plus (Promega, Cat# V6102). Cleavage efficiency was typically ~70–90% at 30 min at room temperature, and showed modest increase

(17) Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron MicroscopyCell Chemical BiologyOctober 1, 2019Daniela Boassa et al.,Life Technologies,Cat# 17504044 GlutaMax,Life Technologies,Cat# 35050061 ProTEV Plus protease,Promega,Cat# V6101 DRAQ5,Cell Signaling Tech.,Cat# 4084 Paraformaldehyde,Electron Microscopy Science,Cat# 19202 Glutaraldehyde,Ted

(18) MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis FailureMolecular cellAugust 22, 2019Wu Z, Tantray I, Lim J, Chen S, Li Y, Davis Z, Sitron C, Dong J, Gispert S, Auburger G, et al.protein (30μl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1× ProTEV digestion buffer (50 mM HEPES pH 7.0,0.5 mM EDTA). The reaction was performed

(19) Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machineryGenes & DevelopmentJuly 1, 2019Marzia Munafò, Vera Manelli, Federica A. Falconio, Ashley Sawle, Emma Kneuss, Evelyn L. Eastwood, Jun Wen Eugene Seah, Benjamin Czech, Gregory J. Hannon100 µL of 1× ProTEV buffer, 1 mM DTT, RNasin Plus (1:50), and 25 U of ProTEV Plus protease (Promega, V6101) and incubated for 2 h at 30°C. Fifteen microliters of Proteinase K in 300 µL of PK/SDS buffer (100

(20) SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/OsterixNature communicationsJuly 19, 2018Fukuda M, Yoshizawa T, Karim MF, Sobuz SU, Korogi W, Kobayasi D, Okanishi H, Tasaki M, Ono K, Sawa T, et al.the resulting E. coli lysate using HaloLink resin (Promega, # G1914). ProTev Plus protease (Promega, #V6101) was used for separation of the SIRT7 protein from Halo-tag. In vitro deacetylation and depropionylation

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ProTEV Plus

ProTEV Plus from Promega

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ProTEV Plus from Promega

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