Elastase V1891 from Promega

Product Specs
ItemElastase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV1891
Quantity5mg
TypeEnzyme

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) The CombE-IDMS Alternate Potency Method for H5N1 and H5N8 Cell-Based VaccinesVaccines (Basel)December 1, 2023Matthew P. Donohue, Zhijun Cao, Thomas Bowen, Robert Dickinson, Ying Zhang, Jiang Qianfor two hours with chymotrypsin (Promega, cat. V1061, Madison, WI, USA) and elastase (Promega, cat. V1891) at ratio of 1:1:2, chymotrypsin:elastase:HA, the same ratio used previously on seasonal quadrivalent

(2) Chemical Proteomic Approach for In-Depth Glycosylation Profiling of Plasma Carcinoembryonic Antigen in Cancer PatientsMolecular & Cellular ProteomicsNovember 1, 2023Jin Chen, Lijun Yang, Chang Li, Luobin Zhang, Weina Gao, Ruilian Xu, Ruijun TianFor multienzymatic digestion, chymotrypsin (catalog no.: V1061; Promega) or elastase (catalog no.: V1891; Promega) was further added according to the instruction of manufacturer. Finally, the peptides were

(3) Mechanisms for Alternaria alternata Function in the Skin During Induction of Peanut Allergy in Neonatal Mice With Skin Barrier MutationsFrontiers in AllergySeptember 6, 2021Lauren M. Buelow, Akihiko Hoji, Kiet Tat, Lindsay M. Schroeder-Carter, Daniela J. Carroll, Joan M. Cook-Millsskin punches were minced and suspended in 5 mL DMEM solution containing 0.5 mg/mL Elastase (Catalog # V1891, Promega), 2.0 mg/mL Collagenase Type XI, and 10 μL/mL DNAse I 37°C with agitation. After 1.5 h, samples

(4) Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cellsCell reportsMay 25, 2021Parker R, Partridge T, Wormald C, Kawahara R, Stalls V, Aggelakopoulou M, Parker J, Powell Doherty R, Ariosa Morejon Y, Lee E, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, Trypsin,Promega,V5111 Elastase,Promega,V1891 PNGaseF,New England Biolabs,P0704S LIVE/DEAD Fixable Aqua Dead Cell Stain Kit,Thermo Fisher Scientific,L34957 ,, Deposited

(5) The Immunomodulatory Metabolite Itaconate Modifies NLRP3 and Inhibits Inflammasome ActivationCell metabolismSeptember 1, 2020Hooftman A, Angiari S, Hester S, Corcoran SE, Runtsch MC, Ling C, Ruzek MC, Slivka PF, McGettrick AF, Banahan K, et al.Coomassie Blue R-250,Cayman,Cat# 14500 Disuccinimidyl Suberate,ThermoFisher,Cat# 21655 Elastase,Promega,Cat# V1891 FuGENE HD Transfection Reagent,Promega,Cat# E2311 Itaconic Acid,Sigma,Cat# I29204 Lipofectamine 2000,ThermoFisher,Cat#

(6) Human Influenza Virus Hemagglutinins Contain Conserved Oligomannose N-Linked Glycans Allowing Potent Neutralization by LectinsCell host & microbeMay 13, 2020Thompson AJ, Cao L, Ma Y, Wang X, Diedrich JK, Kikuchi C, Willis S, Worth C, McBride R, Yates JR 3rd, et al.BP172-5 Iodoacetamide,MilliporeSigma,Cat# I1149 Arg-C (protease),Promega,Cat# V1881 Elastase,Promega,Cat# V1891 Subtilisin,MilliporeSigma,Cat# P5380 Trypsin,Promega,Cat# V5111 Chymotrypsin,Promega,Cat# V1061 Endo H

(7) Genetically encoded protein sulfation in mammalian cellsNature chemical biologyApril 1, 2020Italia JS, Peeler JC, Hillenbrand CM, Latour C, Weerapana E, Chatterjee A.solvent was removed using a SpeedVac for 5 min. Sequencing grade trypsin (V5111) and neutrophil elastase (V1891) was purchased from Promega. Sample was resuspended in either trypsin (for 60 site) or trypsin+elastase

(8) Global site-specific analysis of glycoprotein N-glycan processingNature ProtocolsJune 1, 2018Liwei Cao et al.BP152-10)Calcium chloride dihydrate (CaCl2·2H2O; MilliporeSigma, cat. no. C3306)Elastase (Promega, cat. no. V1891)Subtilisin (MilliporeSigma, cat. no. P5380)Chymotrypsin (Promega, cat. no. V1061)Endo H (New England Biolabs

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Elastase

Elastase from Promega

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Elastase from Promega

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