Asp-N, Sequencing Grade V1621 from Promega

Product Specs
ItemAsp-N, Sequencing Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV1621
Size2microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cryo-EM structures of light chain fibrils from abdominal fat biopsies of multiple myeloma patientsNature CommunicationsFebruary 24, 2026Yuxuan Yao, Shun Yao, Yamei Xu, Qinyue Zhao, Xingyu Xiong, Kaien Liu, Yu Liu, Zhizhi Wang, Wenqing Xu, Cong Liu, et al.sequencing grade modified trypsin (Promega, V5111), chymotrypsin (Promega, V1061), Asp-N (Promega, V1621), proteinase K (Promega, V3021), a-lytic (New England Biolabs, P8113). Peptides were extracted twice

(2) Tailoring a novel colorectal cancer stem cell-targeted therapy by inhibiting the SMYD3/c-MYC axisSignal transduction and targeted therapyJune 30, 2025Lepore Signorile M, Di Nicola E, Forte G, Sanese P, Fasano C, Disciglio V, De Marco K, Latrofa M, De Cecco L, Ficorilli M, et al.(A39271, Thermo Fisher Scientific), and double enzymatic digestion using the endoproteinases AspN (V1621, Promega), which selectively cleaves protein and peptide bonds N-terminal to aspartic acid residues,

(3) Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolismNature Cell BiologyMay 1, 2025Dohun Kim, Rushendhiran Kesavan, Kevin Ryu, Trishna Dey, Austin Marckx, Cameron Menezes, Prakash P. Praharaj, Stewart Morley, Bookyung Ko, Mona H. Soflaee, et al.reagent. Each replicate contained ~5 μg of ACP that was digested overnight with Asp-N peptidase (Promega, V1621) (ACP:Asp-N ratio is 20:1) in 50 mM MOPS pH 7.4 in a 100 μl reaction. The next day, 100 μl of ice-cold

(4) Global analysis of protein and small-molecule substrates of ubiquitin-like proteins (UBLs)Molecular & Cellular ProteomicsApril 18, 2025Guang-Can Shao, Zhen-Lin Chen, Shan Lu, Qing-Cui Wu, Yao Sheng, Jing Wang, Yan Ma, Jian-Hua Sui, Hao Chi, Xiang-Bing Qi, et al.humans. Experimental Procedures Reagents Lys-C (cat. #VA1170), trypsin (cat. #V5280), and Asp-N (cat. #V1621) were purchased from Promega. The anti-HA antibody was purchased from MBL (cat. #M180-3), the anti-GST

(5) O-GlcNAc modification differentially regulates microtubule binding and pathological conformations of tau isoforms in vitroJournal of Biological ChemistryFebruary 3, 2025Mohammed M. Alhadidy, Paul M. Stemmer, Nicholas M. Kanaanfor tandem MS Unmodified and Glc tau proteins were digested using a combination of Asp-N (Promega, #V1621) and rLys-C (Promega, #V167A). First, each recombinant tau protein sample (10 μg, n = 1) was subjected

(6) How J-chain ensures the assembly of immunoglobulin IgM pentamersThe EMBO JournalDecember 4, 2024Chiara Giannone, Xenia Mess, Ruiming He, Maria Rita Chelazzi, Annika Mayer, Anush Bakunts, Tuan Nguyen, Yevheniia Bushman, Andrea Orsi, Benedikt Gansen, et al.39450-01-6 "Trypsin, Sequencing Grade",Merck,Cat #11418475001 "Asp-N, Sequencing Grade",Promega,Cat #V1621 Q5® High-Fidelity DNA Polymerase,New England Biolabs,Cat #M0491S DPNI,New England Biolabs,Cat #R0176S T4 Polynucleotide Kinase

(7) Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profilingnpj Systems Biology and ApplicationsJuly 12, 2024Khang Lê Quý, Maria Chernigovskaya, Maria Stensland, Sachin Singh, Jinwoo Leem, Santiago Revale, David A. Yadin, Francesca L. Nice, Chelsea Povall, Danielle H. Minns, et al.followed by additional Trypsin digestion (Ct + Tryp), and AspN (AspN, Sequencing Grade, Promega Cat V1621). Liquid chromatography and tandem mass spectrometry All mass spectrometry experiments were performed

(8) The interaction between NLRP1 and oxidized TRX1 involves a transient disulfide bondCell Chemical BiologyMay 16, 2024Michael B. Geeson, Jeffrey C. Hsiao, Lydia P. Tsamouri, Daniel P. Ball, Daniel A. Bachovchin,Thermo Scientific,Cat#78430 Sequencing Grade Modified Trypsin,Promega,Cat#V5111 "Asp-N, Sequencing Grade",Promega,Cat#V1621 "Glu-C, Sequencing Grade",Promega,Cat#V1651 Critical commercial assays,Critical commercial assays,Critical

(9) Identification of tyrosine sulfation in the variable region of a bispecific antibody and its effect on stability and biological activitymAbsDecember 31, 2023Christopher B. Lietz, Ekaterina Deyanova, Younhee Cho, Jon Cordia, Sarah Franc, Sally Kabro, Steven Wang, David Mikolon, Douglas D. Bankstrypsin (Promega, Catalog # VA9000), chymotrypsin (Promega, Catalog # V1061) and AspN (Promega, Catalog # V1621) in protease to mAb-X molar ratios of 1:10, 1:20 and 1:50, respectively. Following overnight 37°C incubation

(10) Designer Adaptor Proteins for Functional Conversion of Peptides to Small-Molecule Ligands toward In-Cell Catalytic Protein ModificationACS Central ScienceNovember 22, 2023Akiko Fujimura, Hisashi Ishida, Tamiko Nozaki, Shuhei Terada, Yuto Azumaya, Tadashi Ishiguro, Yugo R. Kamimura, Tomoya Kujirai, Hitoshi Kurumizaka, Hidetoshi Kono, et al.ng/μL Trypsin Gold (Promega, V5280), 10 ng/μL Glu-C (Promega, V1651), and/or 2 ng/μL Asp-N (Promega, V1621) in 50 mM NH4HCO3 aq with 0.02 or 0.03% ProteaseMAX at 37 °C for 3 h to overnight. The digestion enzymes

(11) Identification of a Novel Acid Sphingomyelinase Activity Associated with Recombinant Human Acid CeramidaseBiomoleculesNovember 6, 2023He X, Schuchman EH.ratio of 1:10 (protein/enzyme; w:w) and the other half was digested overnight with Asp-N (Promega, #V1621) at a ratio of 1:25 (protein/enzyme; w:w) at 37 °C. Separation of the peptides was accomplished using

(12) Chloroplasts evolved an additional layer of translational regulation based on non-AUG start codons for proteins with different turnover ratesScientific ReportsJanuary 17, 2023Leelavathi Sadhu, Krishan Kumar, Saravanan Kumar, Abhishek Dass, Ranjana Pathak, Amit Bhardwaj, Pankaj Pandey, Nguyen Van Cuu, Bhupendra S. Rawat, Vanga Siva Reddyfor 15 min, dried, and digested with 50 µl enzyme solution containing 15 ng/μl Asparagine-N (Cat no. V1621, Promega) or Glu-C (Cat no. V165A, Promega) or trypsin in 25 mM NH4HCO3 for 18 to 20 h at 37 °C. Following

(13) PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domainBiochemical JournalSeptember 30, 2022Asad U. Malik, Athanasios Karapetsas, Raja S. Nirujogi, Deep Chatterjee, Toan K. Phung, Melanie Wightman, Robert Gourlay, Nicholas A Morrice, Sebastian Mathea, Stefan Knapp, et al.was purchased from New England Biolabs. Trypsin/Lys-C mix (#V5073), Chymotrypsin (#V1062) and Asp-N (#V1621) were purchased from Promega. Bryostatin-1 (#2383) was purchased from TOCRIS. Recombinant Rab7A protein

(14) TAILS Identifies Candidate Substrates and Biomarkers of ADAMTS7, a Therapeutic Protease Target in Coronary Artery DiseaseMolecular & cellular proteomics : MCPApril 1, 2022MacDonald BT, Keshishian H, Mundorff CC, Arduini A, Lai D, Bendinelli K, Popp NR, Bhandary B, Clauser KR, Specht H, et al.sample was digested with trypsin and the other half was digested with sequencing grade AspN (Promega, V1621) at 37 °C overnight and then mixed together after quenching the reactions the next day. Five percent

(15) Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical localesProceedings of the National Academy of Sciences of the United States of AmericaFebruary 1, 2022Zhao Y, Miranda Herrera PA, Chang D, Hamelin R, Long MJC, Aye Y.Bio-Rad. Proteomics data are provided in a single Excel file (corresponding to Dataset S1). Asp-N (V1621) and Arg-C (V1881) for mass spectrometry experiments were from Promega. Information on cloning primers,

(16) Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptorsiScienceDecember 17, 2021Guo L, Liang Y, Li H, Zheng H, Yang Z, Chen Y, Zhao X, Li J, Li B, Shi H, et al.88805 DL-dithiothreitol,Sigma,Cat# R0861 Iodoacetamide,Sigma,Cat# I10188 Trypsin,Promega,Cat# VA5111 Asp-N,Promega,Cat# V1621 Glu-C,Promega,Cat# V1651 Chymotrypsin,Promega,Cat# V1062 ,, Critical commercial assays,Critical

(17) Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular contextCell metabolismDecember 7, 2021Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, et al.Gel,Sigma-Aldrich,Cat.#A2220 Antimycin A,Sigma-Aldrich,Cat.#A8674 "AspN, Sequencing Grade",Promega,Cat.# V1621 BAMBANKER,Wako,Cat.#306-14684 Bis-Tris,Carl Roth,Cat. #9140.8 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

(18) Photoredox-Catalyzed Decarboxylative C-Terminal Differentiation for Bulk- and Single-Molecule ProteomicsACS Chemical BiologyNovember 19, 2021Le Zhang, Brendan M. Floyd, Maheshwerreddy Chilamari, James Mapes, Jagannath Swaminathan, Steven Bloom, Edward M. Marcotte, Eric V. AnslynThermo Scientific. LysArginase (Cat #EMS0008) was purchased from Millipore Sigma (WI, USA). AspN (Cat#V1621) was purchased from Promega Inc. (Cat #V1621). 2,2,2-trifluoroethanol (Cat #T63002) and tris(2-carboxyethyl)phosphine

(19) Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screenNature communicationsMarch 16, 2021Uliana F, Vizovišek M, Acquasaliente L, Ciuffa R, Fossati A, Frommelt F, Goetze S, Wollscheid B, Gstaiger M, De Filippis V, et al.proteases used in this study were purchased from commercial vendors, Trypsin (V5111), Glu-C (V1651), Asp-N (V1621) and Chymotrypsin (V1091) from Promega (USA) and Lys-C (125-05061) from Wako (Japan). The blood cascade

(20) Protein Turnover in Epithelial Cells and Mucus along the Gastrointestinal Tract Is Coordinated by the Spatial Location and MicrobiotaCell ReportsJanuary 28, 2020Liisa Arike, Andrus Seiman, Sjoerd van der Post, Ana M. Rodriguez Piñeiro, Anna Ermund, André Schütte, Fredrik Bäckhed, Malin E.V. Johansson, Gunnar C. Hansson,Thermo Scientific,24115 DTT,Sigma-Aldrich,D9163 Iodoacetamide,Sigma-Aldrich,I6125 LysC,Wako,125-05061 AspN,Promega,V1621 ,, Deposited Data,Deposited Data,Deposited Data ,, Proteomics data raw data,This paper,ProteomeXchange:

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Asp-N, Sequencing Grade

Asp-N, Sequencing Grade from Promega

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Asp-N, Sequencing Grade from Promega

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