Arg-C, Sequencing Grade V1881 from Promega

Product Specs
ItemArg-C, Sequencing Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV1881
Size10microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Activation of automethylated PRC2 by dimerization on chromatinMolecular CellOctober 17, 2024Paul V. Sauer, Egor Pavlenko, Trinity Cookis, Linda C. Zirden, Juliane Renn, Ankush Singhal, Pascal Hunold, Michaela N. Hoehne-Wiechmann, Olivia van Ray, Farnusch Kaschani, et al.incubation buffer (50 mM Tris-HCl (pH 7.6–7.9), 5 mM CaCl2, 2 mM EDTA) containg 150 ng ArgC (Promega V1881; activated in Arg-C activation buffer (50 mM Tris-HCl (pH 7.6–7.9), 50 mM DTT, 2 mM EDTA). To help bead

(2) Characterization of the Pristionchus pacificus “epigenetic toolkit” reveals the evolutionary loss of the histone methyltransferase complex PRC2GENETICSMay 7, 2024Audrey L Brown, Adriaan B Meiborg, Mirita Franz-Wachtel, Boris Macek, Spencer Gordon, Ofer Rog, Cameron J Weadick, Michael S Werneran endoprotease that cleaves polypeptides after Arg and Lys. Ten micrograms of Arg-C (Promega, cat. #V1881) was resuspended in 10 µl buffer (50 mM ammonium bicarbonate, 5 mM CaCl2, 2 mM EDTA) following the manufacturer’s

(3) Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiencyiScienceApril 19, 2024Benjamin M. Lorton, Christopher Warren, Humaira Ilyas, Prithviraj Nandigrami, Subray Hegde, Sean Cahill, Stephanie M. Lehman, Jeffrey Shabanowitz, Donald F. Hunt, Andras Fiser, et al.,Promega Corporation,https://www.promega.com/products/mass-spectrometry/proteases-and-surfactants/arg_c_-sequencing-grade/?catNum=V1881 Pierce Peptide Retention Time Calibration Mixture,Thermo Fisher Scientific,https://www.thermofisher.com/order/catalog/product/88321 "Formic

(4) Histidine Nτ-methylation identified as a new posttranslational modification in histone H2A at His-82 and H3 at His-39Journal of Biological ChemistrySeptember 1, 2023Takahiro Hayashi, Hiroaki Daitoku, Toru Uetake, Koichiro Kako, Akiyoshi Fukamizuare as follows: trypsin (Promega; V5111), chymotrypsin (Promega; V1061), and clostripain (Promega; V1881). Standards used in LC-MS/MS are as follows: L-lysine monohydrochloride (Sigma-Aldrich; L-5626), Nε-monomethyl-L-lysine hydrochloride

(5) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorbioRxivApril 26, 2023Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.Aldrich,Cat#43815 Iodoacetamide,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research

(6) Histone 4 lysine 5/12 acetylation enables developmental plasticity of Pristionchus mouth formNature communicationsApril 13, 2023Werner MS, Loschko T, King T, Reich S, Theska T, Franz-Wachtel M, Macek B, Sommer RJ.= 5 mM CaCl2, 0.2 mM EDTA, 5 mM DTT), which were digested with 1:50 Arg-C protease (Promega, cat. #V1881): histone protein at 37 °C for 12–16 h. Digest completion was assessed by running an analytical SDS-PAGE

(7) Reductive site-selective atypical C , Z -type/N2-C2 cleavage allows C-terminal protein amidationScience AdvancesApril 8, 2022Tim A. Mollner, Andrew M. Giltrap, Yibo Zeng, Yana Demyanenko, Charles Buchanan, Daniel Oehlrich, Andrew J. Baldwin, Daniel C. Anthony, Shabaz Mohammed, Benjamin G. DavismM EDTA in water) was added [1:10 (v/v)] before ArgC (1 μg; sequencing grade, Promega, catalog no. V1881) was added. The sample was shaken at 37°C (800 rpm) for 20 hours. The reaction was quenched by addition

(8) Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical localesProceedings of the National Academy of Sciences of the United States of AmericaFebruary 1, 2022Zhao Y, Miranda Herrera PA, Chang D, Hamelin R, Long MJC, Aye Y.Proteomics data are provided in a single Excel file (corresponding to Dataset S1). Asp-N (V1621) and Arg-C (V1881) for mass spectrometry experiments were from Promega. Information on cloning primers, shRNA sequences,

(9) The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clustersScience advancesFebruary 1, 2021Schober FA, Moore D, Atanassov I, Moedas MF, Clemente P, Végvári Á, Fissi NE, Filograna R, Bucher AL, Hinze Y, et al.(90056), and Pierce Lys-C, MS-grade (90051) from Thermo Fisher Scientific and Arg-C, sequencing grade (V1881) from Promega. Methylated peptides were enriched with the PTMScan Mono-Methyl Arginine Motif [mme-RG]

(10) Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike proteinSTAR ProtocolsDecember 30, 2020Katelyn E. Rosenbalm, Michael Tiemeyer, Lance Wells, Kazuhiro Aoki, Peng Zhaoreagent",Sigma-Aldrich,320331 Dithiothreitol,Sigma-Aldrich,43815 Iodoacetamide,Sigma-Aldrich,I1149 Trypsin,Promega,V5111 Lys-C,Promega,V1671 Arg-C,Promega,V1881 Glu-C,Promega,V1651 Asp-N,Promega,VA1160 Endoglycosidase H,Promega,V4871 PNGaseF,Promega,V4831 Chymotrypsin,Athens Research

(11) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorCell Host & MicrobeOctober 1, 2020Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.Aldrich,Cat#43815 Iodoacetamide,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research

(12) Human Influenza Virus Hemagglutinins Contain Conserved Oligomannose N-Linked Glycans Allowing Potent Neutralization by LectinsCell host & microbeMay 13, 2020Thompson AJ, Cao L, Ma Y, Wang X, Diedrich JK, Kikuchi C, Willis S, Worth C, McBride R, Yates JR 3rd, et al.A1542 Dithiothreitol (DTT),Fisher,Cat# BP172-5 Iodoacetamide,MilliporeSigma,Cat# I1149 Arg-C (protease),Promega,Cat# V1881 Elastase,Promega,Cat# V1891 Subtilisin,MilliporeSigma,Cat# P5380 Trypsin,Promega,Cat# V5111 Chymotrypsin,Promega,Cat#

(13) Positional effects of premature termination codons on the biochemical and biophysical properties of CFTRThe Journal of physiologyFebruary 1, 2020Yeh JT, Hwang TC.was digested by sequencing-grade modified trypsin (V5111; Promega, WI, USA), sequencing-grade Arg-C (V1881; Promega) or mass spec-grade Lys-C (VA1170; Promega) overnight according to the manufacturer’s protocol.

(14) Mechanism of adrenergic Ca_V1.2 stimulation revealed by proximity proteomicsNatureJanuary 1, 2020Liu G, Papa A, Katchman AN, Zakharov SI, Roybal D, Hennessey JA, Kushner J, Yang L, Chen BX, Kushnir A, et al.detergent-free PBS and subjected to on-bead digest with trypsin (Promega #V5111), LysC (Wako) or ArgC (Promega #V1881, ArgC digestion buffer 50 mM Tris-HCL pH 7.8, 5 mM CaCl2, 2 mM EDTA, 2% acetonitrile (v/v)) separately

(15) RNA exploits an exposed regulatory site to inhibit the enzymatic activity of PRC2Nature Structural & Molecular BiologyMarch 4, 2019Qi Zhang et al.five 8-watt tube lamps. Digestion of the crosslinked ribonucleoprotein complexes by ArgC (Promega #V1881) and LysC (NEB #P8109S) was then performed by adding 5 μL of 100 ng/μL ArgC or LysC stock solution to

(16) Global site-specific analysis of glycoprotein N-glycan processingNature ProtocolsJune 1, 2018Liwei Cao et al.personal protective equipment, including lab coat, gloves, and safety glasses.Arg-C (Promega, cat. no. V1881)Trypsin (Promega, cat. no. V5111)EDTA disodium salt dihydrate (MilliporeSigma, cat. no. E5134)Ethylene

(17) Identification of RNA-binding domains of RNA-binding proteins in cultured cells on a system-wide scale with RBDmapNature ProtocolsDecember 1, 2017Alfredo Castello, Christian K. Frese, Bernd Fischer, Aino I Järvelin, Rastislav Horos, Anne-Marie Alleaume, Sophia Foehr, Tomaz Curk, Jeroen Krijgsveld, Matthias W HentzeD-glucose (Sigma-Aldrich, cat. no. D0422). 8 x Endoproteinase ArgC Sequencing Grade (Promega, cat. no. V1881) 9 x Endoproteinase Lys-C (Wako, cat. no. 129-02541). 10 x Ethylenediaminetetraacetic acid (EDTA)

(18) N-terminal cysteine acetylation and oxidation patterns may define protein stabilityNature communicationsJanuary 1, 0001"Heathcote KC, Keeley TP, Myllykoski M, Lundekvam M, McTiernan N, Akter S, Masson N, Ratcliffe PJ, Arnesen T, Flashman E."Afterwards, 15 mM of DTT was added to quench the remaining CAA and to activate the protease Arg-C (Promega, V1881), which was added in a 1:100 ratio (µg protease/µg protein). Protein samples were digested overnight

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Arg-C, Sequencing Grade

Arg-C, Sequencing Grade from Promega

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Arg-C, Sequencing Grade from Promega

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