Fig 1: Tumor cell‐derived GA‐dependent MIF mediates the formation of an immunosuppressive network. A) PPI network of GA‐related genes. B) MIF was screened as an immune‐related regulator of the reported molecular partner of P115. C) MIF protein expression in BRCA and normal breast tissue in the HPA database. D) Survival curve of OS with high‐ and low‐expression of MIF in BRCA for GSE25037. E) Comparison of immunotherapy predictions between high‐ and low‐ expression of MIF according to the TIDE algorithm in the TCGA‐BRCA cohort. F) Survival curve of DFI after chemotherapy/targeted therapy/immunotherapy/hormone therapy with high‐ and low‐expression of MIF in BRCA. G) MIF‐mediated cell interaction from GSE176068. H) Showing of MIF ligand and receptor pairs (L‐R pairs), with tumor cells as sender and other cell types as receiver from GSE176068. I) Correlation between MIF expression and immune cells in BRCA based on the TIMER database. J) Flow chart of primary cell extraction and culture of multiple immune cells, cytokine‐stimulated differentiation, and exogenous rMIF intervention in immune cell differentiation. K) Flow cytometry statistical results of different immune cell differentiation outcomes induced by different cytokines and rMIF intervention.
Fig 2: Tumor cell GA‐targeting self‐assembled peptide reshapes MIF‐mediated immunosuppressive network. A) Immunohistochemical staining of P115 and MIF after saline, NF‐1, or NF‐2 treatment in the 4T1 tumor‐bearing model. B) Representative immunofluorescence images and C) WB assay of P115 and MIF after PBS, NF‐1, or NF‐2 treatment in 4T1 cells. D) ELISA analysis of secreted MIF of 4T1 cells in the supernatant after different treatments. E) Schematic diagram of co‐culture of 4T1 cells which were treated with peptides to reduce 4T1 cells‐derived MIF or not with different immune cells. F) Representative flow cytometry and corresponding statistical results of CD8+ T cells, mDCs, Macrophages, NK cells, MDSCs, and Tregs. G) Schematic diagram of tumor cell apoptosis and MIF‐mediated immunosuppressive network reshaping induced by tumor cell GA‐targeting self‐assembled peptide NF‐1. Statistical p‐values: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, no significance.
Supplier Page from TargetMol for MIF Protein, Mouse, Recombinant