Fig 1: Renin expression in mouse and human choroid plexus epithelial cells.a, Representative coronal images of mice choroid plexus tissue showing Ren1 mRNA (magenta), aquaporin 1 (AQP1; green), and DAPI (blue). Insets show higher-magnification views of the boxed region. Scale bars, 50 μm; inset, 25 μm. b, Ren1 mRNA expression across age (3, 6, 9, and 12 months) and sex. Data were analyzed by two-way ANOVA followed by Tukey’s post-hoc test (p=0.003; n = 4 – 8 animals per group as indicated). c, Representative images of choroid plexus explants isolated from the lateral ventricle (LV) and fourth ventricle (4V). Higher-magnification views of the boxed regions are shown below. Scale bars, 500 μm (low magnification) and 50 μm (high magnification). d, Representative western blots and quantification of renin protein expression in choroid plexus (ChP) and kidney (Kid) tissue from male and female mice. Recombinant mouse renin was included as a positive control. Two-way ANOVA followed by Uncorrected Fisher’s LSD revealed significant effects of sex (p=0.0266) and tissue type on renin protein abundance (p=0.0099). e,f, Representative coronal images of human choroid plexus showing REN mRNA (magenta) together with aquaporin 1 (AQP1; aqua) (e) or angiotensinogen (AGT; yellow) (f). DAPI is shown in blue. Insets show higher-magnification views of the boxed regions. Scale bars, 50μm; inset, 10μm.
Fig 2: Choroid plexus epithelial cells express various RAS components.a, Pseudobulk analysis of renin expression between species (mice and humans) and sex (female and male), but higher expression in vivo than in vitro. b, Single-cell resolution datasets from mice and humans reveal c, expression of renin (Ren1), angiotensin converting enzyme (Ace), and angiotensinogen (Agt) in CPECs and d, across the different datasets. e, Co-express Ace and Ren1 resulted in a correlation value of 0.04 whereas co-express Agt and Ren1 resulted in a correlation value less than 0.001. f, Ace and Agt expression across 4th ventricle (Ven), lateral ventricles, and 3rd ventricle. g, Single-cell resolution datasets containing at least 3 Ren1+CPECs. h, Approximately 1% of CPECs express renin (total CPECs = 46699; Ren1+ cells = 440) and around 34% express Ace (15824). i, Comparison of renin expressing CPECs between the 4th, lateral, and 3rd ventricles.
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