Fig 1: Identification of a spatially defined NRTN – GRFA2/RET interaction and experimental validation in NB cells. (A) Chord plot showing the number of predicted outgoing signaling interactions from the AC‐like cluster. Number of interactions with NE1 and NE2‐CA and examples of common interactions given. See supplementary material, Table S3 for detailed results. (B) Spatial gene expression plots showing NRTN, GFRA2, and RET expression in sections, as indicated. (C) Bar plot indicating percentages of NRTN expressing primary NB tumors with high (>90th percentile), intermediate, and low (<10th percentile) AC‐like signatures, as indicated. p values calculated using chi‐square test. (D) Kaplan–Meier survival plots comparing overall survival between NB patients with high (>90th percentile), intermediate (10th–90th percentile), and low (<10th percentile) gene expression, as indicated. p values calculated using the log rank test. (E) Western blot showing expression of GFRA2 in four different NB cell lines, as indicated. Normalized densitometry values indicated below each blot. (F, G) Time‐dependent effect of NRTN (100 ng/ml; n = 3) and/or selpercatinib (500 nm; n = 3) application on (F) relative wound density (RWD; measured using a scratch/wound migration assay), and (G) cell growth (measured on Incucyte S3 system) on different NB cell lines, as indicated. p values for indicated timepoints calculated using an unpaired, two‐sided Student's t‐test.