Fig 1: Wnt3a induces formation of an NKD1+ subpopulation with odontoblastic differentiation potential in DPSCs. a Cluster-resolved UMAP of scRNA-seq data from DPSCs treated with blank, Wnt3a, Wnt5a, and Wnt10a under odontogenic conditions. b Bar plot showing the proportional distribution of identified clusters. c GO enrichment analysis of upregulated genes of cluster 3. d FeaturePlot visualization of NKD1 expression pattern. e Immunofluorescence analysis of NKD1 co-localization with DSPP. White arrowheads indicate cells positive for both NKD1 and DSPP. f Flow cytometry quantification of NKD1+DSPP+ cell proportions. (Data are presented as the mean of >3 biological replicates ± SD. ****P < 0.000 1)
Fig 2: Wnt3a induces odontoblastic differentiation of DPSCs. a Uniform manifold approximation and projection (UMAP) of single-cell transcriptomic sequencing data from DPSCs treated with blank, Wnt3a, Wnt5a, and Wnt10a under odontogenic induction conditions. b The correlation heatmap of scRNA-seq datasets. c Volcano plot illustrating the differentially expressed genes (DEGs) of Wnt3a, Wnt5a, and Wnt10a-treated groups compared with control group. d Gene Ontology (GO) enrichment analysis of upregulated DEGs in Wnt3a-treated groups. Alkaline phosphatase (ALP) staining at day 7 (e) and Alizarin Red S (ARS) staining at day 21 (f) post-treatment. g Protein expression and quantitative analysis of odontoblast differentiation-related markers in DPSCs after 7-day treatment. (Data are presented as the mean of >3 biological replicates ± SD. ****P < 0.000 1)
Supplier Page from Abcam for Recombinant Human Wnt10a protein