Fig 1: (a–c) bar plots showing the signal variation for different flow rates (5.4 μL/s and 9.0 μL/s) and several microfluidic channel heights (100 μm, 200 μm, 300 μm) for biotin–streptavidin, Eotaxin, and CD79b interactions, respectively. The Transport Reaction Constant (TRc) values are displayed at the top of each bar to indicate the relationship between reaction rates, film thickness, and mass transport effects.