Fig 1: Expression of four different B7 family members on the cell surface of HNSCC cell lines. (A) Detroit 562 cells were stained with fluorophore-coupled antibodies specifically recognizing VISTA, PD-L2, B7-H2 and B7-H4 and subjected to flow cytometry. Histograms show results of HGF-treated cells (orange), those treated with HGF and Foretinib (blue) or untreated controls (red). Numbers indicate the median of the fluorescence intensity of the corresponding sample. Unstained sample of untreated control is shown for comparison. Relative fluorescence intensity on the x axis is plotted against cell count. (B) Overview of flow cytometry results in all six cell lines. Median values of fluorescence intensity were determined, and median values of corresponding unstained samples were subtracted. Mean values of the median fluorescence intensity of at least three independent experiments were plotted in relation to the untreated control (dashed line). For protein B7-H4, no staining could be determined on the cell surface of all cell lines investigated. The data of this figure can be found in the supplementary materials Table S1. #: no staining, *: p-value < 0.05 in a one-sample t-test.