Fig 1: BCKDK promotes RCC cell proliferation in vitro. A) BCKDK expression in HK2 and six RCC cell lines. B) Overexpression of BCKDK in Caki‐2 and ACHN cells. C) Plate colony formation images and colony count statistics of BCKDK‐overexpressing RCC cells. D) Representative fluorescent images showing EdU‐594 positive cells (red), total nuclei (Hoechst 33342, blue), and merged images of EdU‐tagged vector (control) and BCKDK‐overexpressing RCC cells. E) Soft agar assay of BCKDK‐overexpressing RCC cells. Colony numbers were quantified using Graph Prism 9.0.2 software. F) G. CCK‐8 assay detecting cell viability after BCKDK overexpression in RCC cells.
Fig 2: BCKDK deficiency inhibits RCC tumorigenesis. A) Western blot detection of BCKDK knockdown efficiency in RCC cells. B) Soft agar assay of BCKDK knockdown RCC cells. Colony numbers were quantified using Graph Prism 9.0 software. C) Representative images of plate colony formation and colony count statistics of BCKDK knockdown RCC cells. D) CCK‐8 assay measuring cell viability of BCKDK knockdown cells. E) CCK‐8 assay assessing the cell viability of RCC cells treated with BT2. F) Images of subcutaneous xenografts in mice injected with BCKDK knockdown and control 786‐O cells. G) Tumor volume of xenografted tumors from shCtrl and shBCKDK cells. H) Comparison of tumor weight in the shCtrl and shBCKDK groups at the final time point. I) Representative HE and IHC images of BCKDK and Ki‐67 expression in xenografted tumor tissues. Scale bar: 100 µm.
Fig 3: Schematic diagram of BCKDK promoting RCC progression via AKT‐related signaling pathways.
Supplier Page from Sino Biological, Inc. for BCKDK Protein