Fig 1: Our established workflow for the SLIT2/ROBO1 TR-FRET assay.
Fig 2: (A) The screening funnel for testing the MedChem Express library using our SLIT2/ROBO1 TR-FRET assay. (B) Chemical structure of SMIFH2. (C) Dose-dependent screening of SMIFH2 in the SLIT2/ROBO1 TR-FRET assay. Error bars indicate the standard deviation (n = 3).
Fig 3: Dose-response curve depicting anti-SLIT2 mAb (AB7665) binding in the SLIT2/ROBO1 assay. Error bars indicate the standard deviation (n = 3).
Fig 4: Schematic representation of the TR-FRET assay designed for identifying small molecules that disrupt the SLIT2/ROBO1 interaction.
Fig 5: (A) Scatterplot showing single-dose inhibition data screened at 100 μM of the MedChem Express protein-protein interaction inhibitor library using our SLIT2/ROBO1 TR-FRET assay. (B) Correlation plot of the screened chemical library duplicate runs in the SLIT2/ROBO1 TR-FRET assay.
Supplier Page from Sino Biological, Inc. for Human SLIT2 Protein (His Tag)