Collagenase, Type 4 LS004186 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 4
CompanyWorthington Biochemical Corporation
Price $42.00Supplier Page View Company Product Page
Catalog NumberLS004186
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥160 units per mg dry weight
Size100 mg

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Resolving heterogeneity in Lymph Node Stromal Cells using high-dimensional analysis of non-optimized flow cytometry dataFront BioinformApril 14, 2026Mikala E. Heon, Eduardo Rosa-Molinarbefore transferring it to a tube containing 0.5 mg/mL Collagenase IV (Worthington Biochemical, Cat#LS004186) and 40 μg/mL DNAse 1 (Worthington Biochemical, Cat#LS002138) in αMEM. The tissue was digested at 37

(2) Transcriptional and spatial profiling of fibroblasts from human lungs highlights CTHRC1+ cells as fibrogenic signaling hubs in fibrosisbioRxivApril 10, 2026N.D. Vanegas-Avendano, H. Chen, J. Wellmerling, J. Rodriguez-Lopez, A. Ghobashi, V. Peters, C. Sen, B. F. Reader, K. Shilo, B. Gomperts, et al.(Miltenyi Biotec, 130–096-427) and a solution that included collagenase type IV (Worthington Biochemical, LS004186), elastase (Worthington Biochemical, LS002292), dispase (StemCell, 7913), and DNase I (Worthington Biochemical,

(3) Myofibroblast programming blocks differentiation of TLS-organizing fibroblastic reticular cells in pancreatic cancerCancer CellMarch 26, 2026Elijah Kirschstein, Olivia Harder, Jordan Krull, Madison Sikorski, Shrijan Khanal, Morgan Mack, Carl F. Ware, Elizabeth Evans, Michael J. Gough, Qin Ma, et al.(abraxane),Thermo Fisher Scientific,Cat #328420250 "Collagenase, Type IV",Worthington Biochemical,Cat#LS004186 DNase I,Worthington Biochemical,Cat #LS002004 Bovine serum albumin (BSA),Sigma-Aldrich,Cat #A9418 EDTA,Thermo Fisher Scientific

(4) IL-21 enhances the cytotoxicity of intratumoral CD8+ T cells, improving radiation efficacyJCI InsightFebruary 23, 2026Xin-yang Li, Xue-qi Xie, Bao-chao Wei, Xiao-zheng Sun, Min-xin Chen, Ru-fei Liu, Qing-xu Tao, Yi-heng Huang, Qian Wang, Shuang-shuang Ma, et al.forceps, and subsequently digested with 1 mg/mL collagenase type 4 (Worthington Biochemical Corp., LS004186) and 0.2 mg/mL DNase I (Sigma-Aldrich, DN25) at 37°C for 30 minutes. Digestion was terminated using

(5) Functional human salivary gland organoids for tissue regeneration in chemically defined culture systemsCell Reports MedicineFebruary 17, 2026Xinyuan Zhang, Yingyue Chai, Jiaxuan Qiu, Xiaofeng Shan, Shang Xie, Yi Sui, Zhigang Cai,Worthington Biochemical,Cat#LS004194 Collagenase Type II,Worthington Biochemical,Cat#LS004176 Collagenase Type IV,Worthington Biochemical,Cat#LS004186 A83-01,Sigma-Aldrich,Cat#SML0788 Recombinant human Wnt-3a,Novoprotein,Cat#C18K Recombinant human

(6) Controlling nephron precursor differentiation to generate proximal-biased kidney organoids with emerging maturityNature communicationsAugust 30, 2025Schnell J, Miao Z, Achieng M, Fausto CC, Koppitch K, Takhirov L, Wang V, De Kuyper F, Huang B, Schreiber M, et al.Native Bacillus licheniformis Protease (Creative BioMart, NATE-0633), 2.5 mg/mL collagenase type IV (Worthington Biochemical, LS004186), and 50 µg/mL DNase1 (Worthington Biochemical, LS002058) in 1xPBS−/−. FACS data were processed using

(7) Phosphatase and tensin homolog accelerate the inflammatory injury and glycolysis of chondrocytes through TP53 protein-mediated p38MAPK pathway activation in a high-glucose environmentCytojournalAugust 4, 2025Jian Ding, Chenghua Yang, Xiaofeng Liincubator for 30 min. After the digestion solution was aspirated and discarded, 0.2% collagenase type II (LS004186, Worthington Biochemical Corporation, New Jersey, USA) was added for digestion at 37°C in a constant

(8) Stromal lipid species dictate melanoma metastasis and tropismCancer CellJune 9, 2025Shilpa Gurung, Timothy Budden, Karthik Mallela, Benjamin Jenkins, Alex von Kriegsheim, Esperanza Manrique, David Millán-Esteban, Isabel Romero-Camarero, Fabio Amaral, Sarah Craig, et al.Red mitochondrial superoxide indicator,Thermo Fisher,M36008 Collagenase IV,Worthington Biochemical,LS004186 ONC201,Apex Bio,A8724 N-acetylcysteine,Sigma Aldrich,A0737 Critical commercial assays,Critical commercial

(9) Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancerCell ReportsMay 27, 2025Alan M. Elder, Heather R. Fairchild, Kelsey T. Kines, Lauren M. Cozzens, Alexandria R. Becks, Jill E. Slansky, Steven M. Anderson, Traci R. Lyons(Worthington Biochemical; Cat# LS004174), 500units/ml collagenase IV (Worthington Biochemical; Cat# LS004186), and 20 mg/ml DNase I (Worthington Biochemical; Cat# LS002138) per sample. Samples were incubated at

(10) Gfi1 controls the formation of effector-like CD8+ T cells during chronic infection and cancerNat CommunMay 15, 2025Oluwagbemiga A. Ojo, Hongxing Shen, Jennifer T. Ingram, James A. Bonner, Robert S. Welner, Georges Lacaud, Allan J. Zajac, Lewis Z. Shifine pieces, followed by digestion with 400 µ/mL collagenase D (Worthington Biochemical Corporation, #LS004186) and 20 µg/mL DNase I (Sigma, #10104159001) at 37 °C for 40 min with periodic shaking. EDTA (Sigma,

(11) Stratification of enterochromaffin cells by single-cell expression analysisElifeApril 4, 2025Yan Song, Linda J Fothergill, Kari S Lee, Brandon Y Liu, Ada Koo, Mark Perelis, Shanti Diwakarla, Brid Callaghan, Jie Huang, Jill Wykosky, et al.Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation, LS004186), and 100 U DNase I (Worthington Biochemical Corporation, LS006330) in DMEM/F12 medium for 20–30 min

(12) Protein kinase D1 in myeloid lineage cells contributes to the accumulation of CXCR3+CCR6+ nonconventional Th1 cells in the lungs and potentiates hypersensitivity pneumonitis caused by S. rectivirgulaFrontiers in ImmunologyOctober 11, 2024John D. Snyder, Tae Won Yoon, Sangmin Lee, Priyanka Halder, Elizabeth Ann Fitzpatrick, Ae-Kyung Yiwere perfused through the right ventricle with PBS and digested with collagenase (20 U/mL, type 4, LS004186, Worthington Biochemical Co, Lakewood, NJ) and deoxyribonuclease I (40 μg/mL, LS002004, Worthington Biochemical

(13) Tumor cell–intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenanceJournal of Experimental MedicineOctober 7, 2024Naijun Miao, Dongqing Cao, Jingsi Jin, Guizhi Ma, Haihui Yu, Junwen Qu, Guiping Li, Caixia Gao, Dong Dong, Fan Xia, et al.single-cell suspensions, tumor tissues were cut into small pieces and digested by 1 mg/ml collagenase I (LS004186; Worthington Biochemical) and 0.2 mg/ml DNase I (DN25; Sigma-Aldrich) for 30 min at 37°C. For staining,

(14) Optimized methods for mapping DNA double-strand-break ends and resection tracts and application to meiotic recombination in mouse spermatocytesbioRxivAugust 21, 2024Soonjoung Kim, Shintaro Yamada, Kaku Maekawa, Scott Keeneywithout Ca2+ and Mg2+ and store aliquots at −80 °C.Collagenase IV (Worthington Biochemical, cat. no. LS004186); prepare approximately 10 mg/ml stock solution by add 9 ml of GBSS to 100 mg powder and store aliquots

(15) NAD(+) metabolism enzyme NNMT in cancer-associated fibroblasts drives tumor progression and resistance to immunotherapy by modulating macrophages in urothelial bladder cancerJournal for immunotherapy of cancerJuly 27, 2024Yang M, Wang B, Hou W, Zeng H, He W, Zhang XK, Yan D, Yu H, Huang L, Pei L, et al.collagenases (collagenase I, LS004194, 0.17 mg/mL, collagenase II, LS004174, 0.056 mg/mL, collagenase IV, LS004186, 0.17 mg/mL, Worthington Biochemical Corporation, Lakewood, New Jersey, USA), deoxyribonuclease l (LS002138,

(16) Olfactory neuroblastoma mimics molecular heterogeneity and lineage trajectories of small-cell lung cancerCancer cellJune 10, 2024Finlay JB, Ireland AS, Hawgood SB, Reyes T, Ko T, Olsen RR, Abi Hachem R, Jang DW, Bell D, Chan JM, et al.(0.25%) (Thermo Fisher cat# 25200–072), 600 μL of collagenase type 4 (Worthington Biochemical cat# LS004186) from a 10 mg/mL stock prepared in HBSS without calcium and magnesium (Thermo Fisher cat# 14025), and

(17) Hematopoietic stem cell niche generation and maintenance are distinguishable by an epitranscriptomic programCellMay 23, 2024Gao L, Lee H, Goodman JH, Ding L.proteins","Chemicals, peptides, and recombinant proteins" Collagenase IV,Worthington Biochemical,Cat#LS004186 DNase I,MilliporeSigma,Cat#260913 Calcein-AM,BioLegend,Cat#425201 Liberase,Roche,Cat#5401119001 OCT

(18) Assessing the combined impact of fatty liver-induced TGF-β1 and LPS-activated macrophages in fibrosis through a novel 3D serial section methodologyScientific reportsMay 18, 2024Ishiyama S, Hayatsu M, Toriumi T, Tsuda H, Watanabe K, Kasai H, Kishigami S, Mochizuki K, Mikami Y.using flow cytometry. Liver samples were minced and incubated with 10 U/mL Collagenase (CLS4 Type IV, #LS004186, Worthington Biochemical Corporation, Lakewood, NJ) and 50 U/mL DNase I (#2270A, Takara Bio, Shiga Japan)

(19) Hepatic stellate cell-intrinsic role of SOCS1 in controlling hepatic fibrogenic response and the pro-inflammatory macrophage compartment during liver fibrosisFrontiers in ImmunologyOctober 4, 2023Rajani Kandhi, Mehdi Yeganeh, Akihiko Yoshimura, Alfredo Menendez, Sheela Ramanathan, Subburaj Ilangumaranwas digested by perfusion with freshly prepared Type IV collagenase (Worthington Biochemical; Cat # LS004186; 18 mg of 325 collagen digestion units) in 50 mL of prewarmed HHBSS containing 1.5 mM CaCl2 (wash buffer).

(20) Intertumoral lineage diversity and immunosuppressive transcriptional programs in well-differentiated gastroenteropancreatic neuroendocrine tumorsScience AdvancesSeptember 29, 2023Samantha E. Hoffman, Todd W. Dowrey, Carlos Villacorta Martin, Kevin Bi, Breanna Titchen, Shreya Johri, Laura DelloStritto, Miraj Patel, Colin Mackichan, Stephanie Inga, et al.cold enzymatic dissociation mix [collagenase type 4 (100 μg/ml; Worthington Biochemical Corporation, #LS004186) and deoxyribonuclease I (100 μg/ml; StemCell Technologies, #07900) in RPMI + Hepes (Thermo Fisher Scientific,

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Collagenase, Type 4 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 4 from Worthington Biochemical Corporation

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