Nuclease, Micrococcal LS004797 from Worthington Biochemical Corporation

Product Specs
ItemNuclease, Micrococcal
CompanyWorthington Biochemical Corporation
Price $94.00Supplier Page View Company Product Page
Catalog NumberLS004797
Source/Expression SystemStaphylococcus aureus (Strain Foggi)
StorageStore at 2-8°C
Activity≥6,000 units per mg protein
Size15 ku

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg ExtractsGenes CellsApril 27, 2026Keishi Shintomi, Keita Ohsumi, Mari Iwabuchi, Tatsuya HiranomM Tris–HCl, 5 mM CaCl2, pH 7.9) and treated with micrococcal nuclease (Worthington Biochemical Co., LS004797) at 10, 50, and 250 units/mL for 5 min. Digestion was terminated by mixing the reaction mixture with

(2) Enhancer hubs govern chromatin topology and Th17 cell identitybioRxivApril 8, 2026Keith Siklenka, Chuangchuang Zhang, Liqing Li, Morgan E Parker, Naren U Mehta, Alejandro Barrera, Revathy Venukuttan, Gregory E Crawford, Charles A Gersbach, Maria Ciofani, et al.with few modifications. Specifically, we empirically determined MNase (Worthington Biochemical, Cat #LS004797) chromatin fragmentation conditions for Naïve and Th17 cells to be 2U MNase for 12 minutes at 37C per

(3) Urine cf-nucleosomes: A non-invasive window into human physiology and diseaseCell GenomicsOctober 8, 2025Matan Lotem, Israa Sharkia, Batia Azria, Esther Harpenas, Maayan Ormianer, Hadar Rosen, Tal Falick-Michaeli, Nir Friedmanβ-mercaptoethanol, 500 μM spermidine]. Chromatin was digested with Micrococcal nuclease [Worthington Biochemical LS004798] at 5 units per 1 million cells. Chromatin was collected by centrifugation (14,000xg, 10 min at 4C)

(4) The histone chaperone Spt6 controls chromatin structure through its conserved N-terminal domainMolecular CellSeptember 18, 2025James L. Warner, Vanda Lux, Václav Veverka, Fred WinstonNuclease,Thermo,88701 Zymolyase 100T,US Biological,Z1004 MNase,Worthington Biochemical Corporation,LS004797 Exonuclease III,NEB,M0206 RNase A/T1,Thermo,EN0551 Proteinase K,Roche,3115887001 "Histone H3 (recombinant,

(5) Divergent Mechanisms of H2AZ.1 and H2AZ.2 in PRC1-Mediated H2A UbiquitinationCellsJuly 23, 2025Xiangyu Shen, Chunxu Chen, Amanda E. Jones, Xiaokun Jian, Gengsheng Cao, Hengbin Wangpreviously described protocol [45]. Briefly, 2 × 108 cells were digested with 20 U of micrococcal nuclease (LS004797, Worthington Biochemical Corporation, Lakewood, NJ, USA) for 1 h to obtain mono-nucleosomes. Nucleosomes

(6) LDB1 establishes multi-enhancer networks to regulate gene expressionMolecular CellJanuary 16, 2025Nicholas G. Aboreden, Jessica C. Lam, Viraat Y. Goel, Siqing Wang, Xiaokang Wang, Susannah C. Midla, Alma Quijano, Cheryl A. Keller, Belinda M. Giardine, Ross C. Hardison, et al.on ice. Chromatin from permeabilized nuclei was digested with 10 U MNase (Worthington Biochemical, LS004798) for 10 min at 37C with 850rpm rotation. Digested fragments were de-phosphorylated with 5 U r-SAP (New England Biolabs

(7) Putative looping factor ZNF143/ZFP143 is an essential transcriptional regulator with no looping functionMolecular cellJanuary 2, 2025Narducci DN, Hansen AS.crosslinked chromatin was digested to mononucleosomes using micrococcal nuclease (Worthington Biochemical # LS004798) at a concentration determined by titration. Biotin-dATP and biotin-dCTP were incorporated into the

(8) Interplay between Two Paralogous Human Silencing Hub (HuSH) Complexes in Regulating LINE-1 Element SilencingNature CommunicationsNovember 3, 2024Zena D. Jensvold, Julia R. Flood, Anna E. Christenson, Peter W. Lewiscocktail, and 0.5 mM PMSF), and treated with 105 Units of MNase (Worthington Biochemical Corporation, LS004798) per 40 million cells for 10 min at 37 °C. Reaction was quenched by adding 10 mM EDTA, 5 mM EGTA, 80

(9) YY1-controlled regulatory connectivity and transcription are influenced by the cell cycleNature GeneticsSeptember 1, 2024Jessica C. Lam, Nicholas G. Aboreden, Susannah C. Midla, Siqing Wang, Anran Huang, Cheryl A. Keller, Belinda Giardine, Kate A. Henderson, Ross C. Hardison, Haoyue Zhang, et al.ice. Nuclei were pelleted, resuspended in MB 1 and treated with 10 U MNase (Worthington Biochemical, LS004797) for 10 min at 37 °C. MNase was titrated such that chromatin was digested to a 9:1 monomer:dimer ratio.

(10) Plasticity of the mitotic spindle in response to karyotype variationCurrent BiologyAugust 5, 2024Preethi Kunchala, Joseph M. Varberg, Eileen O’Toole, Jennifer Gardner, Sarah E. Smith, Melainia McClain, Sue L. Jaspersen, R. Scott Scott Hawley, Jennifer L. Gerton41103 5-Fluoroorotic Acid,Zymo Research,Cat# F9001 Micrococcal nuclease,Worthington Biochemical,Cat# LS004798 Propidium iodide solution,Sigma-Aldrich,Cat# P4864 Triton X-100,Sigma-Aldrich,Cat# T9284 NP-40 Alternative,Sigma-Aldrich,Cat#

(11) Comparison of cell response to chromatin and DNA damageNucleic Acids ResearchNovember 27, 2023Artyom Luzhin, Priyanka Rajan, Alfiya Safina, Katerina Leonova, Aimee Stablewski, Jianmin Wang, Denisha Robinson, Natalia Isaeva, Omar Kantidze, Katerina GurovaInc.). MNase-sequencing Micrococcal nuclease (MNase) was purchased from Worthington Biochemical Corp (Lakewood, NJ, Cat# LS004797). MNase digestion was done as described (32) followed by the analysis of fragment length by BioAnalyzer

(12) Measuring occupancies of the nucleosome and nucleosome-interacting factors in vivo in Saccharomyces cerevisiae genome-wideMethodsOctober 1, 2023Emily Biernat, Uzair Khan, Chhabi K. GovindMicrococcal nuclease (MNase, Stock: 45 kU; Worthington Biochemical, Lakewood NJ, USA, catalog number LS004798. To reconstitute MNase, mix 1.6 ml glycerol with 3.5 ml NP-S buffer, place the tube on the nutator until

(13) Lola-I is a promoter pioneer factor that establishes de novo Pol II pausing during developmentNature CommunicationsSeptember 21, 2023Vivekanandan Ramalingam, Xinyang Yu, Brian D. Slaughter, Jay R. Unruh, Kaelan J. Brennan, Anastasiia Onyshchenko, Jeffrey J. Lange, Malini Natarajan, Michael Buck, Julia Zeitlingerhomogenizer, then digested with a concentration gradient of MNase (Worthington Biochemical Corporation #LS004798) in NPS buffer for 30 min at 37 °C. All digestion concentrations were run on a gel and the concentration

(14) Global histone protein surface accessibility in yeast indicates a uniformly loosely packed genome with canonical nucleosomesEpigenetics & ChromatinDecember 1, 2021Luke T. Marr, Josefina Ocampo, David J. Clark, Jeffrey J. Hayessplit into two aliquots for digestion with either 480 or 960 units of MNase (Worthington Biochemical LS004798) at room temperature for 3 min. The amount of enzyme was empirically determined to yield predominantly

(15) Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organismsSTAR ProtocolsJune 1, 2021Laura E. McKnight, Johnathan G. Crandall, Thomas B. Bailey, Orion G.B. Banks, Kona N. Orlandi, Vi N. Truong, Drake A. Donovan, Grace L. Waddell, Elizabeth T. Wiles, Scott D. Hansen, et al.252549 Zymolyase (R) 100T,AMS Bio,Cat# 120493-1 "Nuclease, micrococcal",Worthington Biochemical,Cat# LS004798 Exonuclease III,New England Biolabs,Cat# M0206S RNase A,Fisher BioReagents,Cat# BP25391 Proteinase

(16) Basis of specificity for a conserved and promiscuous chromatin remodeling proteineLifeFebruary 12, 2021Drake A Donovan, Johnathan G Crandall, Vi N Truong, Abigail L Vaaler, Thomas B Bailey, Devin Dinwiddie, Orion GB Banks, Laura E McKnight, Jeffrey N McKnight,Proteoloc protease inhibitor cocktail (EDTA-free),Expedeon,Cat# 44214, "Chemical compound, drug","Nuclease, micrococcal",Worthington Biochemical,Cat# LS004798, "Chemical compound, drug",Zymolyase (R) 100T,AMS Bio,Cat# 120493-1, "Chemical compound, drug",Agencourt

(17) TATA and paused promoters active in differentiated tissues have distinct expression characteristicsMolecular Systems BiologyFebruary 5, 2021Vivekanandan Ramalingam, Malini Natarajan, Jeff Johnston, Julia Zeitlingerreplicate and then digested with a concentration gradient of MNase (Worthington Biochemical Corporation #LS004798) for 30 min at 37°C. All samples were run on a gel, and the digestion concentration to be sequenced

(18) A method for assessing histone surface accessibility genome-wideMethodsDecember 1, 2020Luke T. Marr, David J. Clark, Jeffrey J. Hayeswill yield predominantly mononucleosomes (~150 bp). Micrococcal nuclease (Worthington Biochemical; LS004798) is prepared as in [28] to (10 units/μl). MNase is then added in the appropriate amounts and samples

(19) H3 K27M and EZHIP Impede H3K27-Methylation Spreading by Inhibiting Allosterically Stimulated PRC2Molecular CellNovember 1, 2020Siddhant U. Jain et al.beta-mercaptoethanol) and treated with 750 units of Micrococcal Nuclease, MNase (Worthington Biochemical Corporation, LS004798) for 20 min at 37°C. MNase digestion was quenched and nuclear extract was prepared by adding 10 mM EDTA,

(20) S-phase-independent silencing establishment in Saccharomyces cerevisiaeeLifeJuly 20, 2020Goodnight D, Rine J.samples were incubated at 37°C for 5 min, followed by addition of 4 U MNase (Worthington Biochemical LS004798; Lakewood, NJ) per 1 × 107 cells. Samples were placed on an end-over-end rotator at 37°C for 20 min,

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Nuclease, Micrococcal from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Nuclease, Micrococcal from Worthington Biochemical Corporation

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