Neutral Protease (Dispase), Purified LS02104 from Worthington Biochemical Corporation

Product Specs
ItemNeutral Protease (Dispase), Purified
CompanyWorthington Biochemical Corporation
Price $352.00Supplier Page View Company Product Page
Catalog NumberLS02104
Source/Expression SystemBacillus polymyxa
StorageStore at 2-8°C
Activity≥4 units per mg dry weight
Size50 mg

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) The gain-of-function TREM2-T96K mutation increases risk for Alzheimer’s disease by impairing microglial functionNeuronJanuary 7, 2026Dominika J. Pilat, Hoang Le, Dmitry Prokopenko, Chih-Chung Jerry Jerry Lin, William A. Eimer, Luisa Quinti, Evan P. Gavrilles, Sheyla N. Garcia, Sara N. Heitman, Alzheimer’s Disease Neuroimaging Initiative (ADNI), et al.GentleMACS™ C-tubes (#130–093-237; Miltenyi Biotech) with pre-warmed RPMI 1640 containing Dispase (2 U/ml, LS02104; Worthington Biochemical Corporation) and Collagenase Type 3 (200 U/ml, # LS004182; Worthington Biochemical Corporation

(2) Protocol for generation of immortalized retinal, choroid, and brain endothelial cell linesSTAR ProtocolsDecember 1, 2025Laura Hannig, Jakob S. Bernhard, Wolfgang Lezuo, Robin Heiden, Mario Vallon, Süleyman Ergün, Carola Y. Förster, Anja Schlecht, Barbara M. Braungeranimal-free recombinant protein (VEGFa)",PeproTech,450-32 Neutral protease (Dispase),Worthington Biochemical,LS02104 Phosphate-buffered saline (PBS) - powder,A. Hartenstein GmbH,"A0964,9100" Polybrene,Merck KGaA (Millipore),TR-1003-G ,, Experimental models

(3) The recycling collagen receptor uPARAP is a unique mediator of stromal drug delivery to carcinoma cellsMolecular cancer therapeuticsJuly 16, 2025Nørregaard KS, Larsen IME, Jürgensen HJ, Blomquist MH, Çakılkaya P, Metrangolo V, Perlado AM, Krigslund O, Gårdsvoll H, Poulsen TT, et al.cat. no. LS002006, Worthington Biochemical), neutral protease (dispase, CAS no. 9001-92-7, cat. no. LS02100, Worthington Biochemical), BSA (cat. no A3059, CAS no. 9048-46-8, Sigma-Aldrich), protease inhibitor

(4) Protocol for isolation of endothelial cells from adult mouse brainSTAR ProtocolsJune 20, 2025Audrey G. Miglietta, Jennifer David-Bercholz, Roeben N. Munji, Robert S. Pulido, Allison L. Soung, Niccolò Terrando, Richard Daneman, Ting Yangremoval beads II∗,Miltenyi Biotec,Cat#130-096-433 Neutral protease (250 mg)∗,Worthington Biochemical,Cat#LS02106 PDS kit (5 vi) (papain)∗,Worthington Biochemical,Cat#LK003178 Sodium pyruvate (100 mM),Invitrogen,Cat#11360-070 TRIzol,Invitrogen,Cat#15-596-018 "Trypsin

(5) Single cell profiling of human airway identifies tuft-ionocyte progenitor cells displaying cytokine-dependent differentiation bias in vitroNat CommunJune 4, 2025Viral S. Shah, Avinash Waghray, Brian Lin, Atharva Bhagwat, Isha Monga, Michal Slyper, Bruno Giotti, Sunghyun Kim, Dawei Sun, Ke Xu, et al.containing collagenase (225 units/ml Worthington LS004188), dispase (2.5 units/ml Worthington Biochemical LS02100), elastase (2 units/ml Worthington Biochemical LS002279), pronase (1 mg/ml Roche11 459 643 001), DNAse

(6) R and S enantiomers of CBD3063, a CaV2.2 N-type calcium channel modulator, alleviate capsaicin-induced inflammatory painNeurobiol PainMay 16, 2025Santiago Loya-López, Erick J. Rodríguez-Palma, Aida Calderón-Rivera, Kimberly Gomez, Samantha Perez-Miller, Rajesh KhannaCat. No. LS004194, Worthington Biochemical, Lakewood, NJ) and neural protease (1.04 mg/mL; Cat. No. LS02104, Worthington Biochemical, Lakewood, NJ) for 50 min at 37 °C with gentle agitation. Subsequently, the

(7) Sympathetic Neurons Promote Small Cell Lung Cancer Through the Beta-2 Adrenergic ReceptorCancer DiscoveryMarch 3, 2025Tala FNU, Peiguo Shi, Wanwei Zhang, Sanny S.W. Chung, Christopher B. Damoci, Yinshan Fang, Qi-Yue Chen, Anjali Saqi, Yuefeng Huang, Xuebing Wu, et al.25200–056), type 4 collagenase (Worthington Biochemical, LS004188) and dispase (Worthington Biochemical, LS02104) in HBSS-free medium (Gibco, 14175–095) at 37°C for 30 min with intermittent pipetting. Cell suspensions

(8) Tissue origin of endothelial cells determines immune system modulation and regulation of HIF-1α-, TGF-β-, and VEGF signalingiScienceFebruary 21, 2025Robin Heiden, Laura Hannig, Jakob S. Bernhard, Mario Vallon, Anja Schlecht, Nico Hofmann, Süleyman Ergün, Franziska Hoschek, Maximilian Wagner, Andreas Neueder, et al.collagenase, type 4 (Worthington Biochemical, LS004188)) + 1 U/ml neutral protease (Worthington Biochemical, LS02104) + 5 U/ml DNase I (Worthington Biochemical, LS006342) by triturating with a 200 μL pipette tip (100

(9) Targeting Nav1.7 and Nav1.8 with a PIKfyve inhibitor to reverse inflammatory and neuropathic painNeurobiology of PainJanuary 1, 2025Erick J. Rodríguez-Palma, Santiago Loya-Lopez, Sophia M. Min, Aida Calderon-Rivera, Kimberly Gomez, Rajesh Khanna, Alison D. AxtmanCat. No. LS004194, Worthington Biochemical, Lakewood, NJ) and neural protease (1.04 mg/mL; Cat. No. LS02104, Worthington Biochemical, Lakewood, NJ) for 50 min at 37 °C with gentle agitation. Subsequently, the

(10) A Pvr–AP-1–Mmp1 signaling pathway is activated in astrocytes upon traumatic brain injuryeLifeOctober 31, 2024Tingting Li, Wenwen Shi, Margaret S Ho, Yong Q Zhangtube (1.5 mL) containing 200 µL AHS with Pronase (1 mg/mL; P5147, Sigma-Aldrich) and Dispase (1 mg/mL; LS02104, Worthington Biochemical Corporation). After tissue digestion for 30 min at 25°C, the enzyme medium

(11) Microglia are not necessary for maintenance of blood-brain barrier properties in health, but PLX5622 alters brain endothelial cholesterol metabolismNeuronSeptember 4, 2024Caterina P. Profaci, Sean S. Harvey, Kaja Bajc, Tony Z. Zhang, Danielle A. Jeffrey, Alexander Z. Zhang, Kelsey M. Nemec, Hayk Davtyan, Carleigh A. O’Brien, Gabriel L. McKinsey, et al.LS003089 Collagenase Type 2,Worthington Biochemical,Cat. # LS004176 Neutral Protease,Worthington Biochemical,Cat. # LS02104 ACK Lysis Buffer,Quality Biological,Cat. # 118-156-101 Myelin removal beads,Miltenyi Biotec,Cat. #

(12) The Transcriptional Landscape of Pericytes in Acute Ischemic StrokeTranslational Stroke ResearchAugust 1, 2024Carolina Buizza, Andreas Enström, Robert Carlsson, Gesine Paul(400 U/ml, Worthington Biochemical, cat. LS004188), dispase I (1.2 U/ml, Worthington Biochemical, cat. LS02104), and DNAse I (32 U/ml, Thermo Fisher Scientific, cat. 18047019) in 6 ml phosphate buffer saline (PBS)

(13) Heterogeneous fibroblasts contribute to fibrotic scar formation after spinal cord injury in mice and monkeysNature CommunicationsJuly 27, 2024Xiaoyu Xue, Xianming Wu, Yongheng Fan, Shuyu Han, Haipeng Zhang, Yuting Sun, Yanyun Yin, Man Yin, Bing Chen, Zheng Sun, et al.Then 1.0 mg/mL collagenase type II (LS004176, Worthington Biochemical) and 0.4 mg/mL neutral protease (LS02104, Worthington Biochemical) were added, and the sample was incubated for 30 min at at 37 °C with slow

(14) Vhl safeguards thymic epithelial cell identity and thymopoietic capacity by constraining Hif1a activity during developmentiScienceJuly 19, 2024Christiane Grammer, Julia A. Komorowska, Jeremy B. Swann,Worthington Biochemical Corporation,Cat#LS004189 neutral protease (dispase),Worthington Biochemical Corporation,Cat#LS02104 DNaseI,Roche,Cat#11284932001 EDTA,Carl Roth,Cat#8043.1 T7 RNA polymerase,Roche,Cat#10881767001 SP6 RNA polymerase

(15) Olfactory neuroblastoma mimics molecular heterogeneity and lineage trajectories of small-cell lung cancerCancer cellJune 10, 2024Finlay JB, Ireland AS, Hawgood SB, Reyes T, Ko T, Olsen RR, Abi Hachem R, Jang DW, Bell D, Chan JM, et al.calcium and magnesium (Thermo Fisher cat# 14025), and 600 μL of dispase (Worthington Biochemical cat# LS02104). Enzymatic digestion was quenched on ice with 500 μL of quench media containing 7.2 mL of Leibovitz’s

(16) Single-cell and single-nucleus RNA-sequencing from paired normal-adenocarcinoma lung samples provide both common and discordant biological insightsPLOS GeneticsMay 30, 2024Sébastien Renaut, Victoria Saavedra Armero, Dominique K. Boudreau, Nathalie Gaudreault, Patrice Desmeules, Sébastien Thériault, Patrick Mathieu, Philippe Joubert, Yohan BosséDNase I 100 μg/mL (Sigma Aldrich, cat. 11284932001), Dispase 100 μg/mL (Worthington Biochemical, cat. LS02100), Collagenase A 1500 μg/mL (Sigma Aldrich, cat.10103578001) and Collagenase IV 100 μg/mL (Worthington

(17) Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regenerationeLifeNovember 21, 2023Laura Celotto, Fabian Rost, Anja Machate, Juliane Bläsche, Andreas Dahl, Anke Weber, Stefan Hans, Michael BrandµM" "Chemical compound, drug",Neutral protease (Dispase),Worthington Biochemical Corporation,Catalog #LS02100,0.2 U/ml Commercial assay or kit,Papain dissociation System,Worthington Biochemical Corporation,Catalog

(18) A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stagesNeural DevelopmentAugust 24, 2022Marc Corrales, Benjamin T. Cocanougher, Andrea B. Kohn, Jason D. Wittenbach, Xi S. Long, Andrew Lemire, Albert Cardona, Robert H. Singer, Leonid L. Moroz, Marta Zlaticplaced in 18 μL of AHS on ice. Once all samples were prepared, 2 μL of 10 × neutral protease (Cat #: LS02100, Worthington Biochemical Corp, Lakewood, NJ, USA) was added to a final volume of 20 μL. The intact brain

(19) A-type FHFs mediate resurgent currents through TTX-resistant voltage-gated sodium channelseLifeApril 20, 2022Yucheng Xiao, Jonathan W Theile, Agnes Zybura, Yanling Pan, Zhixin Lin, Theodore R Cummins,Worthington Biochemical,Cat# LS004194, "Chemical compound, drug",Neutral protease,Worthington Biochemical,Cat# LS02104, "Software, algorithm",PulseFit,HEKA,, "Software, algorithm",PCLAMP,Molecular Devices,, "Software,

(20) ASCL1 represses a SOX9⁺ neural crest stem-like state in small cell lung cancerGenes & developmentJune 1, 2021Olsen RR, Ireland AS, Kastner DW, Groves SM, Spainhower KB, Pozo K, Kelenis DP, Whitney CP, Guthrie MR, Wait SJ, et al.HBSS with calcium and magnesium (Thermo Fisher 14025), and 600 μL of dispase (Worthington Biochemical LS02104). Enzymatic digestion was quenched on ice with 500 μL of quench media containing 7.2 mL of Leibovitz's

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Neutral Protease (Dispase), Purified from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Neutral Protease (Dispase), Purified from Worthington Biochemical Corporation

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