Collagenase, Type 2 LS004176 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 2
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004176
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) The cysteine metabolic regulation through CSE-transsulfuration pathway is essential in iron-induced oxidative damage of heartRedox RepDecember 31, 2026Yong Zhang, Yijun Xin, Jie Zhou, Guang Yang, Siying Liwere first excised, then minced with scissors, and digested using a pre-prepared collagenase type 2 (LS004176, Worthington Biochemical) stock solution (pre-digestion for 5 min, followed by formal digestion for

(2) FAK modulates immune response and fibroblast activation in biomaterial-induced fibrosisBiomaterialsJuly 1, 2026Marc A. Fernández-Yagüe, Graham F. Barber, Aránzazu del Campo, Andrés J. GarcíaU/mL Dispase II (ThermoFisher, 17105041), 0.2 % Type II Collagenase (Worthington Biochemical Corp., LS004176) at 37 °C for 1 h. The digested tissue suspension was then passed through a 35 μm cell strainer (Falcon,

(3) COX2 + / PDGFRα + fibroblasts selectively localize near bile ducts and interact with immune cells in early liver fibrosis in micePhysiological ReportsMay 24, 2026Momo Goto, Tamaki Kurosawa, Hiroyuki Koike, Noriyuki Kaji, Ichiro Manabe, Taiki Mihara, Takashi Chaen, Yumiko Oishi, Masatoshi Horiinto the heart, and the liver was perfused with 20 mL of pre‐warmed (37°C) 0.05% collagenase type 2 (# LS004176, Worthington Biochemical Corporation, Lakewood, NJ, USA) in DMEM at a controlled flow rate not exceeding

(4) Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male miceBone ResApril 28, 2026Monica Pata, Diep Ngoc Thi Pham, Julie Lacombe, B. Ashok Reddy, Young Woong Kim, Abeer Gamal Ali Ahmed, Monzur Murshed, Mathieu Ferrondigested at 37 °C twice for 10 min and twice for 30 min with a solution of collagenase type 2 (0.1 mg/mL; LS004176, Worthington Biochemical Corporation, Lakewood, NJ) containing 0.25% trypsin. Only the last two digestions

(5) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,CellROX Orange Flow Cytometry Assay Kit,Invitrogen,C10493, "Chemical compound, drug",Collagenase Type 2,Worthington Biochemical Corporation,LS004176, "Chemical compound, drug",Dispase,Worthington Biomedical,LS02109, "Chemical compound, drug",Deoxyribonuclease,Worthington

(6) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,CellROX Orange Flow Cytometry Assay Kit,Invitrogen,C10493, "Chemical compound, drug",Collagenase Type 2,Worthington Biochemical Corporation,LS004176, "Chemical compound, drug",Dispase,Worthington Biomedical,LS02109, "Chemical compound, drug",Deoxyribonuclease,Worthington

(7) IRF9-dependent transcriptional regulation of SLC40A1 suppresses ferroptosis in dilated cardiomyopathyScientific ReportsApril 27, 2026Zhi Hu, Tingzhong Wang, Qun Lu, Qiang Song, Jianhua Huoenzymatic digestion (15–20 min each, 37 °C) with collagenase II (250 U/mL; Worthington Biochemical, Cat# LS004176) and pancreatin (60 µg/mL; Sigma-Aldrich, Cat# P3292). Supernatant from each round was collected into

(8) Circulating cytokines as triggers of endothelial dysfunction and sex-specific interstitial cell response in fibrocalcific aortic valve diseaseInt J Biol SciApril 23, 2026Vincenza Valerio, Francesca Bertolini, Valentina Rusconi, Mattia Chiesa, Ilaria Massaiu, Paola Gripari, Valentina Mantegazza, Cristina Gatto, Michele Ciccarelli, Arianna Galotta, et al.12491023) containing 2 mg/mL type II collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA; Cod: LS004176), 10% fetal bovine serum (FBS, Microtech, Naples, Italy; Cod: RM10432) and 1% penicillin-streptomycyn

(9) Apolipoprotein C3 drives adverse cardiac remodeling in ischemic heart failureBMC MedicineApril 9, 2026Yufei Han, Yixue Zhao, Zihao Zhou, Changyun Liu, Jinxuan Chen, Guolin Miao, Ling Zhang, Yuhui Wang, Jianping Li, Wei Huang, et al.underwent sequential enzymatic digestion cycles (6 min/cycle) in 5 mL of buffer [0.6 mg/mL Collagenase II (LS004176, Worthington Biochemical Corporation) and 1 mg/mL pancreatin (T8150, Solarbio) dissolved in sterile

(10) Inhibition of adenylyl cyclase 1 or exchange protein activated by cAMP restores ATP‐sensitive potassium channel activity after chronic opioid exposureBritish Journal of PharmacologyApril 7, 2026Amanda H. Klein, S. M. Sabbir Alam, Kayla Johnson, Christian Kriner, Brie Beck, Bethany Nelson, Cassidy Hill, Belle Meyer, Jonas Mellang, Val J. Wattsincubated with papain (LS003126, Worthington Biochemical, Lakewood, NJ, USA) and then a collagenase (LS004176, Worthington Biochemical) solution in HBSS for 10 min at 37°C. DRG were triturated with EMEM media (#10–010-CV,

(11) TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regenerationCommunications BiologyApril 2, 2026Neena Lala-Tabbert, Allan Humphrey, Diana Ratsun, Nathalie Earl, Martine St-Jean, Eric C. LaCasse, Robert G. Kornelukof C57BL/6 wild-type and TWEAK KO were dissected and digested in a 0.2% Collagenase Type 2 solution (LS004176, Worthington Biochemical). Cells were washed with serum-free high glucose DMEM and then enriched for

(12) Role of A-delta low threshold mechanoreceptors and tropomyosin receptor kinase B plasticity in at-level aversive pain after spinal cord injuryExperimental NeurologyApril 1, 2026Shangrila Parvin, Kyeongran Jang, Sandra M. Garrawaywith Dispase II (2.5 μ/mL; #04942078001, Sigma-Aldrich, St. Louis, MO) and collage-nase (200 μ/mL; #LS004176, Worthington Biochemical, Lakewood, NJ), dissociated cells were seeded at low density on 22 × 22 mm

(13) Semi-automated isolation of parenchymal and non-parenchymal liver cells from mice and humans with enhanced stellate cell fractionCell & BioscienceMarch 14, 2026Anne Dropmann, Bedair Dewidar, Kerry Gould, Raquel L. Baccetto, Christoph Meyer, Tiziana Caccamo, Pia Erdoesi, Şamil Ayvaz, Andrea Scheffschick, Georg Damm, et al.at 7 ml/min, followed by collagenase II buffer (Worthington Biochemical Corporation, USA, cat. no. LS004176) at 4 ml/min for approximately 5 min each in healthy livers or 8 min each in injured livers. To relieve

(14) Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shiftsCell MetabolismMarch 10, 2026Snigdha Tiash, Saket Awadhesbhai Patel, Marjori Russo, Anusha Suresh, Bo Pang, Yun-Ling Pai, Wentong Jia, Rachael L. Field, Terri Pietka, Nada A. Abumrad, et al.11088858001 Collagenase A,Millipore Sigma,Cat # C5138 Collagenase Type 2,Worthington Biochemical,Cat# LS004176 "Dispase II (neutral protease, grade II), Roche",Millipore Sigma,Cat# 4942078001 DNaseI,Millipore

(15) Anti-angiogenic effects of p,p’-Dichlorodiphenyldichloroethylene (p,p’-DDE) in the human microvasculatureToxicology in VitroMarch 1, 2026Bulbul Ahmed, Melissa G. Farb, Kimberly Chen, Luise Pernar, Brain Carmine, Donald T. Hess, Jennifer Schlezinger, Noyan Gokceminced using scissors and digested with 1 mg/mL collagenase type II (Worthington Biochemical, Cat No. LS004176) for 15 to 30 minutes at 37°C in a shaking water bath. After digestion, the tissue suspension was filtered

(16) Functional human salivary gland organoids for tissue regeneration in chemically defined culture systemsCell Reports MedicineFebruary 17, 2026Xinyuan Zhang, Yingyue Chai, Jiaxuan Qiu, Xiaofeng Shan, Shang Xie, Yi Sui, Zhigang Caiproteins" ,, Collagenase Type I,Worthington Biochemical,Cat#LS004194 Collagenase Type II,Worthington Biochemical,Cat#LS004176 Collagenase Type IV,Worthington Biochemical,Cat#LS004186 A83-01,Sigma-Aldrich,Cat#SML0788 Recombinant

(17) Subcellular proteomic analyses reveal REEP5 knockdown in the mouse heart disrupts mitochondrial networksMolecular & Cellular ProteomicsFebruary 9, 2026Michelle Di Paola, Cristine J. Reitz, Uros Kuzmanov, Kateleen Jia, Anthony O. Gramolinisubsequently digested with pre-warmed collagenase type II (475 U/mL; Worthington Biochemical Corporation, #LS004176) buffer containing blebbistatin (Cayman Chemical Corporation, #13013). The cell suspension was passed

(18) Stimulator of Interferon Genes Is a Fine-Tuner, but Not a Prime Mover, of Kidney InflammationThe American Journal of PathologyFebruary 1, 2026Madiha Zahra Syeda, Emily S.H. Yeung, Lisa Y.Q. Hong, Suzanne L. Advani, Youan Liu, Laurette Geldenhuys, Ferhan S. Siddiqi, Veera Ganesh Yerra, Sri Nagarjun Batchu, Andrew Advanipenicillin-streptomycin, before perfusion with 20 mL digestion buffer containing collagenase type II (catalog number LS004176; lot number 40H20247; Worthington Biochemical Corp., Lakewood, NJ) in phosphate-buffered saline. Both

(19) The ubiquitin ligase KLHL6 drives resistance to CD8+ T cell dysfunctionNatureJanuary 14, 2026Hongcheng Cheng, Yapeng Su, Xiaoli Pan, Yue Xu, Ermei Xie, Jing Du, Daniel G. Chen, Xiaomeng Dai, Raphael Gottardo, Philip D. Greenberg, et al.flow cytometry on day 4 post-ACT. The tumours were digested by Type II collagenase (Worthington Biochemical, LS004176) and processed with Percoll (Cytiva, 17089109). Adoptively transferred OT-I T cells were isolated from

(20) KIF13B Attenuates Sepsis-Induced Myocardial Dysfunction through the Stabilization of PLIN5Research (Wash D C)January 12, 2026Lianxin Zhang, Guolin Miao, Si Mei, Yufei Han, Yitong Xu, Wenxi Zhang, Jingxuan Chen, Kaikai Lu, Yinqi Zhao, Zihao Zhou, et al.underwent sequential enzymatic digestion cycles (6 min/cycle) in 5 ml of buffer (0.6 mg/ml collagenase II [LS004176, Worthington Biochemical Corporation‌] and 1 mg/ml pancreatin [T8150, Solarbio] dissolved in sterile

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Collagenase, Type 2 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 2 from Worthington Biochemical Corporation

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