Collagenase, Type 2 LS004176 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 2
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004176
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size1 gm

Add to Compare List

Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) The cysteine metabolic regulation through CSE-transsulfuration pathway is essential in iron-induced oxidative damage of heartRedox RepDecember 31, 2026Yong Zhang, Yijun Xin, Jie Zhou, Guang Yang, Siying Liwere first excised, then minced with scissors, and digested using a pre-prepared collagenase type 2 (LS004176, Worthington Biochemical) stock solution (pre-digestion for 5 min, followed by formal digestion for

(2) Cytoplasmic RBM20 granules modulate non-RBM20 target splicing through interactions with other splicing factorsNucleic Acids ResAugust 4, 2026Yanghai Zhang, Zachery R Gregorich, Chunling Liu, Eli J Larson, Ying Ge, Wei GuoNext, hearts were perfused with digestion buffer [300 U/ml, 50 ml/heart of collagenase type II (cat# LS004176, Worthington Biochemical Corporation, Lakewood, NJ, USA) in perfusion buffer] and the calcium concentration

(3) Clinical OCT computation-modeled prognosis of coronary plaque progression with validation by fusogenic macrophage nano-targetingNano ConvergJuly 30, 2026Sewoom Baek, Hyun-Su Ha, Seyong Chung, Ji Youn Lee, Hyeongyun Choi, Chansik Kim, Seok Joon Lee, Seul-Gee Lee, Yong-Joon Lee, Jung-Sun Kim, et al.in 10 mL RPMI 1640 medium (11,875,093, Gibco, Waltham, MA, USA) with 1 mg mL⁻1 collagenase type II (LS004176, Worthington Biochemical Corporation, NJ, USA) without FBS for 30 min under gentle shaking. Enzymatic

(4) CD157+ vascular endothelial stem cells represent a conserved subpopulation with an angiogenic gene expression profileStem Cell ReportsJuly 14, 2026Tomohiro Iba, Taku Wakabayashi, Rie Ito, Ai Sugawara, Satoshi Fujimura, Mika Sawane, Kazuaki Yoshioka, Aya Matsui, Jun-ichi Morishige, Naoto Nagata, et al.,Thermo Fisher Scientific,Cat# 17105041 Collagenase,Wako,Cat# 034-22363 Collagenase Type II,Worthington Biochemical,Cat# LS004176 Collagenase type XI,Sigma-Aldrich,Cat# H3506 Soybean trypsin inhibitor,Worthington Biochemical,Cat#

(5) Ginsenoside Rb1-Enriched Saponin Fraction Inhibits M1 Macrophage Polarization by Suppression of TLR4 Trafficking in Metabolic Dysfunction-Associated Alcoholic Liver DiseaseNutrientsJuly 13, 2026Tae-Un Kim, Jae-Hyuk Yim, Woo Jun Kim, Seoung-Woo Lee, Hee-Yeon Kim, Kyung-Ku Kang, Min-Soo Seo, Man Hee Rhee, Su-Min Baek, Seong-Kyoon Choi, et al.previous study [20,27]. Liver perfusion was conducted using 1× EGTA buffer and collagenase type II (LS004176; Worthington Biochemical Corporation, Lakewood, NJ, USA) to obtain liver cell suspensions. Perfusion

(6) Aging Enhances Serotonergic Signaling via 5-HT7 Receptors Underlying Mechanical AlloknesisAging CellJuly 13, 2026Qiaofeng Zhao, Alberto Leguina-Ruzzi, Mitsutoshi Tominaga, Yayoi Kamata, Atsuko Kamo, Huiying Wan, Bin Yin, Yuping Ran, Kenji Takamoricolumns of C57BL/6J mice under sterile conditions and enzymatically dissociated using collagenase (LS004176, Worthington Biochemical Corporation, Columbus, OH, USA), L‐cysteine (C7352‐25, Sigma‐Aldrich, St. Louis,

(7) Structural and functional benchmarking of monolayer- and bioreactor-generated hiPSC-derived cardiomyocytesAPL BioengJuly 9, 2026Yongjun Jang, Kevin Shani, Anna Clouvel-Gervaiseau, Maksymilian Prondzynski, Simone Nuebling, Christopher J. Shin, Yichong Wang, Giulio Ciucci, Michio Kawai, Yoonseo Lee, et al.changed every 2 days until day 15. On day 15, 2D-Mono was dissociated with 300 U/ml of collagenase (#LS004176, Worthington Biochemical Corp.) (30 min in incubator), filtered (70 μm), and cryopreserved in STEMdiff™ Cardiomyocyte Freezing Medium

(8) Tunable Self-Assembling Cellular Microarray for Single-Neutrophil Vital and Suicidal Extracellular TrapsLab on a ChipJuly 6, 2026Jacob Doon-Ralls, Sophia Mayone, Xilal Y. Rima, Dharti Shantaram, Kim Truc Nguyen, Ajeet Singh, Bradley J. Needleman, Sabrena Noria, Stacy Brethauer, Kyle A. Perry, et al.BP1605-100; 5 mM adenosine, Thermo Scientific A10781.09; and collagenase II, Worthington Biochemical LS004176) for 45 minutes at 37 °C. Digested AT was filtered through 500 μm and 300 μm filters, respectively,

(9) FAK modulates immune response and fibroblast activation in biomaterial-induced fibrosisBiomaterialsJuly 1, 2026Marc A. Fernández-Yagüe, Graham F. Barber, Aránzazu del Campo, Andrés J. GarcíaU/mL Dispase II (ThermoFisher, 17105041), 0.2 % Type II Collagenase (Worthington Biochemical Corp., LS004176) at 37 °C for 1 h. The digested tissue suspension was then passed through a 35 μm cell strainer (Falcon,

(10) FGF13 Deficiency Ameliorates Paclitaxel‐Induced Neuropathic Pain by Inhibiting VASH1‐Mediated Microtubule Detyrosination to Promote MitophagyAdvanced ScienceJune 18, 2026Yiming Dong, Yidan Wang, Simeng Lv, Zishan Dong, Kaixi Zhi, Xiuhua Guo, Xuyan Li, Ruoxi Yu, Yiyi Zhang, Siyuan Cheng, et al.Balanced Salt Solution supplemented with collagenase Type II (2.5 mg/mL, Worthington Biochemical, USA; LS004176) and Dispase (7.5 mg/mL, Sigma‐Aldrich, St. Louis, MO, USA; 04942078001), followed by incubation at

(11) Mitochondrial protein OPA3 sustains cardiac function by regulating calcium handling in male miceNature CommunicationsJune 17, 2026Na Geng, Taiwei Chen, Hao Li, Shan Lu, Yuehong Wang, Ling Gao, Donald M. Bers, Xiyuan Luthe heart. The aortic arch was cannulated using a 20 G needle and perfused with type II collagenase (LS004176; Worthington Biochemical Corp., USA) solution (1 mg/ml) for 10 min. The digested ventricles were pulled

(12) COX2 + / PDGFRα + fibroblasts selectively localize near bile ducts and interact with immune cells in early liver fibrosis in micePhysiological ReportsMay 24, 2026Momo Goto, Tamaki Kurosawa, Hiroyuki Koike, Noriyuki Kaji, Ichiro Manabe, Taiki Mihara, Takashi Chaen, Yumiko Oishi, Masatoshi Horiinto the heart, and the liver was perfused with 20 mL of pre‐warmed (37°C) 0.05% collagenase type 2 (# LS004176, Worthington Biochemical Corporation, Lakewood, NJ, USA) in DMEM at a controlled flow rate not exceeding

(13) Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male miceBone ResApril 28, 2026Monica Pata, Diep Ngoc Thi Pham, Julie Lacombe, B. Ashok Reddy, Young Woong Kim, Abeer Gamal Ali Ahmed, Monzur Murshed, Mathieu Ferrondigested at 37 °C twice for 10 min and twice for 30 min with a solution of collagenase type 2 (0.1 mg/mL; LS004176, Worthington Biochemical Corporation, Lakewood, NJ) containing 0.25% trypsin. Only the last two digestions

(14) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,CellROX Orange Flow Cytometry Assay Kit,Invitrogen,C10493, "Chemical compound, drug",Collagenase Type 2,Worthington Biochemical Corporation,LS004176, "Chemical compound, drug",Dispase,Worthington Biomedical,LS02109, "Chemical compound, drug",Deoxyribonuclease,Worthington

(15) IRF9-dependent transcriptional regulation of SLC40A1 suppresses ferroptosis in dilated cardiomyopathyScientific ReportsApril 27, 2026Zhi Hu, Tingzhong Wang, Qun Lu, Qiang Song, Jianhua Huoenzymatic digestion (15–20 min each, 37 °C) with collagenase II (250 U/mL; Worthington Biochemical, Cat# LS004176) and pancreatin (60 µg/mL; Sigma-Aldrich, Cat# P3292). Supernatant from each round was collected into

(16) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,CellROX Orange Flow Cytometry Assay Kit,Invitrogen,C10493, "Chemical compound, drug",Collagenase Type 2,Worthington Biochemical Corporation,LS004176, "Chemical compound, drug",Dispase,Worthington Biomedical,LS02109, "Chemical compound, drug",Deoxyribonuclease,Worthington

(17) Circulating cytokines as triggers of endothelial dysfunction and sex-specific interstitial cell response in fibrocalcific aortic valve diseaseInt J Biol SciApril 23, 2026Vincenza Valerio, Francesca Bertolini, Valentina Rusconi, Mattia Chiesa, Ilaria Massaiu, Paola Gripari, Valentina Mantegazza, Cristina Gatto, Michele Ciccarelli, Arianna Galotta, et al.12491023) containing 2 mg/mL type II collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA; Cod: LS004176), 10% fetal bovine serum (FBS, Microtech, Naples, Italy; Cod: RM10432) and 1% penicillin-streptomycyn

(18) SLC1A5 prevents aortic aneurysm and dissection by glutaminolytic-epigenetic orchestration of vascular smooth muscle cell homeostasisNature CommunicationsApril 15, 2026Pinglian Yang, Zhechang Gao, Weile Ye, Chunhong Zhou, Zhihua Zheng, Meiming Su, Yu He, Zhuoming Li, Jaroslav Pelisek, Ke He, et al.removed the adventitia and scraped off endothelium after treating with collagenase type II (1 mg/mL, LS004176, Worthington Biochemical Corporation, NJ, USA) in Hank’s balanced salt solution (CR14175, Cienry, Zhejiang,

(19) Apolipoprotein C3 drives adverse cardiac remodeling in ischemic heart failureBMC MedicineApril 9, 2026Yufei Han, Yixue Zhao, Zihao Zhou, Changyun Liu, Jinxuan Chen, Guolin Miao, Ling Zhang, Yuhui Wang, Jianping Li, Wei Huang, et al.underwent sequential enzymatic digestion cycles (6 min/cycle) in 5 mL of buffer [0.6 mg/mL Collagenase II (LS004176, Worthington Biochemical Corporation) and 1 mg/mL pancreatin (T8150, Solarbio) dissolved in sterile

(20) Inhibition of adenylyl cyclase 1 or exchange protein activated by cAMP restores ATP‐sensitive potassium channel activity after chronic opioid exposureBritish Journal of PharmacologyApril 7, 2026Amanda H. Klein, S. M. Sabbir Alam, Kayla Johnson, Christian Kriner, Brie Beck, Bethany Nelson, Cassidy Hill, Belle Meyer, Jonas Mellang, Val J. Wattsincubated with papain (LS003126, Worthington Biochemical, Lakewood, NJ, USA) and then a collagenase (LS004176, Worthington Biochemical) solution in HBSS for 10 min at 37°C. DRG were triturated with EMEM media (#10–010-CV,

Be the First to Write a Review!

Collagenase, Type 2 from Worthington Biochemical Corporation

Description

Worthington Biochemical Corporation

Citations (211)

Reviews

Collagenase, Type 2 from Worthington Biochemical Corporation

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map