Collagenase, Type 2 LS004177 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 2
CompanyWorthington Biochemical Corporation
Price $900.00Supplier Page View Company Product Page
Catalog NumberLS004177
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size5 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) A Simplified Langendorff-Based Method for Mouse Cardiac Myocyte IsolationBio ProtocJuly 20, 2026Mie S. Larsen, Morten B. Thomsen, Tamzin Zawadzki(Le Genest-Saint-Isle, France) Reagents 1. Collagenase II (Worthington Biochemical, catalog number: LS004177) Note: Store as a lyophilized powder at 2–8 °C. Stable for long-term storage when kept dry and protected

(2) Structural and functional benchmarking of monolayer- and bioreactor-generated hiPSC-derived cardiomyocytesAPL BioengJuly 9, 2026Yongjun Jang, Kevin Shani, Anna Clouvel-Gervaiseau, Maksymilian Prondzynski, Simone Nuebling, Christopher J. Shin, Yichong Wang, Giulio Ciucci, Michio Kawai, Yoonseo Lee, et al.overnight in 1 mg/ml trypsin at 4 °C with gentle agitation, followed by 1 mg/ml of collagenase II (#LS004177, Worthington Biochemical Corp., Lakewood, NJ) dissociation at 37 °C with several rounds of trituration.

(3) YAP1 Knockdown Reduces IL-1β-Induced Human Chondrocyte Inflammation and Promotes Human MSC ChondrogenesisPharmaceuticals (Basel)May 29, 2026Liru Wen, Sibylle Grad, Laura B. Creemers, Martin J. StoddartTGF-β1 protein (Cat# 30R-AT072) were supplied by Fitzgerald (New York, NY, USA). Collagenase II (Cat# LS004177) was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA). Lipofectamine™ RNAiMAX Transfection Reagent

(4) Boosting Sensory Nerve‐to‐Bone Interactions Enhances Hedgehog Mediated Calvarial Bone RepairAdvanced ScienceApril 20, 2026Zhao Li, Xin Xing, Beicheng Du, Myles Zhou, Austin Z. Chen, Mary Archer, Chunbao Rao, Manyu Zhu, Masnsen Cherief, Aaron W. JamesLakewood, NJ, USA, LS004197) and collagenase type II (1 mg/mL; Worthington Biochemical Corporation, LS004177). Cell fractions (from sequential digestions 1–6) were collected and resuspended in red blood cell lysis

(5) The Secretome From Mechanically Loaded Myoblasts Enhances Tenocyte-Mediated Wound Healing in a 3D In Vitro Tendon ModelFASEB JMarch 31, 2026Junhong Li, Paula Milena Giraldo-Osorno, Christer Thomsen, Pernilla Eliasson, Ludvig J. BackmanDenmark, 21041025) supplemented with 1 mg/mL type II collagenase (Worthington Biochemical, NJ, USA, LS004177), 20% FBS (Thermo Fisher Scientific, A5256801), and 1% penicillin–streptomycin (Pen Strep; Thermo Fisher Scientific

(6) Respiratory viral infections prime accelerated lung cancer growthCellMarch 11, 2026Wei Qian, Xiaoqin Wei, Andrew J. Barros, Xiangyu Ye, Haibo Zhang, Qing Yu, Samuel P. Young, Eric V. Yeatts, Yury Park, Chaofan Li, et al.tlrl-b5lps AddaVax,InvivoGen,Cat# vac-adx-10 "Collagenase, Type 2",Worthington Biochemical Corporation,Cat# LS004177 Fc Receptors Blocker,Innovex,Cat# NB309 Fetal Bovine Serum,Cytiva,Cat# SH30070.03 RPMI 1640 Medium,Gibco,Cat#

(7) The Secretome From Mechanically Loaded Myoblasts Enhances Tenocyte‐Mediated Wound Healing in a 3D In Vitro Tendon ModelFASEB JMarch 9, 2026Junhong Li, Paula Milena Giraldo‐Osorno, Christer Thomsen, Pernilla Eliasson, Ludvig J. BackmanDenmark, 21041025) supplemented with 1 mg/mL type II collagenase (Worthington Biochemical, NJ, USA, LS004177), 20% FBS (Thermo Fisher Scientific, A5256801), and 1% penicillin–streptomycin (Pen Strep; Thermo Fisher Scientific

(8) The asymmetrical ROS–METTL3–ESR1 axis in paraspinal muscle progenitor cells determines the progression of adolescent idiopathic scoliosisExperimental & Molecular MedicineMarch 5, 2026Bin Li, Amila Kuati, Wenyuan Sui, Jinkui Cai, Tianyuan Zhang, Yaolong Deng, Junlin Yang, Xingzuan Lin, Xiexiang Shaoenzymatically dissociated using collagenase II and dispase (Worthington Biochemical, 700-800 U/ml, cat. no. LS004177; Life Technologies, 11 U/ml, cat. no. 17105-041). The resulting digest was passed through a 40-μm cell

(9) Aryl hydrocarbon receptor activation alters emergency hematopoiesis during influenza A virus infectionToxicological SciencesFebruary 18, 2026Kristina N Fenner, Alicia M Healey, Anthony M Franchini, Craig N Morrell, B Paige Lawrenceinstilled with 1 ml RPMI 1640 containing 1 mg/ml collagenase type 2 (Worthington Biochemical Corp, #LS004177) and 30 µg/ml DNAse I (Sigma, #10104159001) via the trachea. To prevent backflow and loss of cellular

(10) LncRNA HOXC-AS3 prevents chondrocyte senescence and osteoarthritis progression through miR-615-3p sponging and RRBP1 interactionCell & BioscienceFebruary 15, 2026Chan Wang, Jun Zhou, Lulu Chen, Changyan Ma, Zhan Dong, Dengshun Miaocartilage tissues were subjected to enzymatic digestion with 0.2% collagenase II (Worthington Biochemical, LS004177) for 12 h at 37 °C as previously described [14]. Cell culture and treatments The human chondrocyte

(11) Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co‐Culture SystemCell ProliferationJanuary 1, 2026Baochen Bai, Jiting Li, Ze Wang, Yuhan Yang, Jieqing He, Gonglie Chen, Yufan Zhang, Yan Qi, Zhongjun Wan, Lin Cai, et al.digested with 5 mL lysis buffer containing 0.1% trypsin (T8150, Solarbio) and 0.056% collagenase type II (LS004177, Worthington Biochemical) for 4 min at 37°C. The above steps were repeated 10–13 times until the hearts

(12) Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responsesCellDecember 24, 2025Diana Castaño, Emily Bettini, Binod Kumar, Aleksey Chudnovskiy, Anna Siv, Giulia Protti, Sandra Nakadakari-Higa, Simona Ceglia, Nina De Luna, Joy E. Chiu, et al.Pharma Solutions,N/A CL264,InvivoGen,Cat#tlrl-c264e-5 Collagenase II,Worthington Biochemical,Cat#LS004177 cOmplete protease inhibitor cocktail tables,Roche,Cat#11697498001 Cytofix/Cytoperm,BD Biosciences,Cat#554655 Dispase,Sigma

(13) MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor and its 3D orientation in cellsNature CommunicationsDecember 21, 2025Alexander H. Clowsley, Anna Meletiou, Radoslav Janicek, Alexandre F. E. Bokhobza, Evelina Lučinskaitė, Gabriela Bleuer, Isabelle Jansen, Peter P. Jones, William E. Louch, Christian Soellerisolation buffer which included collagenase type II (160 U/mL, Worthington Biochemical Corporation, LS004177) and protease type XIV (0.21 U/mL, Sigma, P5147) for ~15 min. After digestion the heart was cut down

(14) Sympathetic nerve inhibition enhances calvarial bone repair via senescent macrophage-induced osteogenesis and angiogenesisCell Death DiscovDecember 10, 2025Lei Zhao, Zhaoning Xu, Peiru Zhao, Zhiying Pang, You You, Chao Wu, Di Zhu, Meiling Su, Ning Zhang, Jian Luo, et al.of collagenase Type I/II (1 mg/mL, Worthington Biochemical Corporation, Lakewood, NJ; LS004197 and LS004177) and Dispase II (2 mg/mL) for three rounds of 15 min each. Three animals were included in each group:

(15) Tanshinone IIA suppresses fibro-adipogenic progenitor differentiation and attenuates fat infiltration in rotator cuff injury via Wnt/β-catenin pathwayRegenerative therapyDecember 1, 2025Zhang Y, Li X, Zhang R, Wang M, Wang T, Liu G, Kuati A, Mao W.minced into 1–2 mm3 pieces. Then 700 U/ml collagenase II (Worthington Biochemical Corporation, cat#LS004177) and 11U/ml dispase (Life Technologies, cat#17105-041) were used to digest these minced pieces. The

(16) Respiratory viral infections prime accelerated lung cancer growthbioRxivSeptember 11, 2025Wei Qian, Xiaoqin Wei, Andrew J Barros, Xiangyu Ye, Qing Yu, Samuel P Young, Eric V Yeatts, Yury Park, Chaofan Li, Gislane Almeida-Santos, et al.E. coli O55:B5,InvivoGen,Cat# tlrl-b5lps "Collagenase, Type 2",Worthington Biochemical Corporation,Cat# LS004177 Fc Receptors Blocker,Innovex,Cat# NB309 Fetal Bovine Serum,Cytiva,Cat# SH30070.03 RPMI 1640 Medium,ThermoFisher Scientific

(17) The air pollutant phenanthrene disrupts calcium cycling and alters repolarization in female sheep ventricular cardiomyocytesPhysiol RepAugust 26, 2025C. R. Marris, S. N. Kompella, A. W. Trafford, J. C. Hancox, H. A. Shielscalcium‐free solution containing the digestive enzymes collagenase type II (0.096 mg.mL−1, cat no: LS004177, Worthington Biochemical Corporation) and protease type XIV (0.02 mg.mL−1, cat no: P5147, Sigma‐Aldrich)

(18) Protocol for in situ transplantation of adipose stem cells into mouse epididymal white adipose tissueSTAR ProtocAugust 15, 2025Xiyan Liao, Qin Zeng, Haowei Zhang, Limin Xie, Tuo DengExpress,Gibco,Cat# 12604039 Matrigel,Corning,Cat# 356237 Collagenase II,Worthington Biochemical,Cat# LS004177 Isoflurane solution,RWD Life Science,Cat# R510-22-10 ,, Experimental models: Organisms/strains,Experimental models

(19) Unravelling the Role of PIEZO1 in Adipogenesis of Fibrogenic/Adipogenic Progenitors for Muscle Fat Infiltration After Rotator Cuff TearJ Cachexia Sarcopenia MuscleJuly 29, 2025Xingzuan Lin, Qingfa Song, Amila Kuati, Zhanghua Li, Hao Zhou, Shujing Feng, Minhui Wang, Siyuan Zhu, Guoqing Cui, Jianhua Wang, et al.mechanically minced and digested with collagenase II (Worthington Biochemical, 700–800 U/mL, Cat. No. LS004177) for 1 h. Subsequently, the muscle fragments were treated with a mixture of collagenase II (70–80 U/mL)

(20) Tumour initiated purinergic signalling promotes cardiomyocyte RBFOX1 degradation and cardiotoxicity from DNA damaging anticancer agentsNat CommunJuly 25, 2025Saymon Tejay, Maria Areli Lorenzana-Carrillo, Guocheng Huang, Seyed Amirhossein Tabatabaei Dakhili, Yuan -Yuan Zhao, Farah Eaton, Michelle Mendiola Pla, Dawn E. Bowles, Adam Kinnaird, D. Ian Paterson, et al.control and transgenic mice by digestion with type II collagenase (Worthington Biochemical Corporation, LS004177) using a modified Langendorff perfusion apparatus as previously established8. Briefly, after heparin

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Collagenase, Type 2 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 2 from Worthington Biochemical Corporation

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