Collagenase, Type 1 LS004196 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 1
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004196
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) The Mitochondrial NAD Transporter SLC25A51 in Adipocytes Regulates Adipose Tissue Mitochondrial Function and Systemic Metabolism During AgingAging CellApril 21, 2026Daiki Kojima, Keisuke Yaku, Shotaro Kosugi, Ryunosuke Mitsuno, Kenji Kaneko, Yoshinaga Kawano, Akihito Hishikawa, Seiya Mizuno, Manami Katoh, Akiko Satoh, et al.Isolation Adipose tissue samples were incubated in Krebs Ringer buffer containing 1 mg/mL of collagenase type I (#LS004196; Worthington Biochemical, Lakewood, NJ) at 37°C for 45 min. After collagenase digestion, adipocytes

(2) Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon RepairJournal of Orthopaedic ResearchApril 7, 2026Varun Arvind, Elaine Nagahara, Hui Zhang, Peter Shyu, X. Edward Edward Guo, Alice H. HuangFlow cytometry Dissected tendons were incubated in 5 mg/mL Collagenase I (Worthington Biochemical, cat # LS004196) and I mg/mL Collagenase IV (Worthington Biochemical, cat # LS004188) for 4 hours at 37°C on a rocker.

(3) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.digested with Collagenase Type I and Collagenase Type IV (Worthington Biochemical Corporation, cat. nos. LS004196 and LS004188) for 30 min at 37 °C with shaking at 125 rpm. Digestion was quenched with ice-cold PBS

(4) The uncoordinated‐5 homologue A is a key receptor in netrin‐ligand‐mediated fast‐twitch myotube formation in male micePhysiological ReportsFebruary 17, 2026Takahiro Maeno, Tomoki Ushijima, Koichi Ojima, Yohei Ogawa, Sayuki Hayashi, Hikaru Imakyure, Rika Osaki, Ryuki Oyama, Aoi Ogawa, Akimasa Takano, et al.Soleus and EDL muscles were dissected from mice and treated with 0.5% (w/v) type I collagenase (cat. no. LS004196, Worthington Biochemical, Freehold, NJ, USA) in Dulbecco's modified Eagle's medium (DMEM; cat. no. 0845816,

(5) The mechanosensitive channel TRPV4 inhibits pulmonary inflammation by limiting NF-κB signaling in alveolar macrophagesScience SignalingDecember 23, 2025Adam M. Boulton, Megan E. Grund, Yuxin Wang, Erica M. Orsini, Yan Liu, Susamma Abraham, Lisa M. Grove, Ryan Musich, Caitlin M. Snyder, Haley Ricci, et al.with cold PBS. Lung tissue was digested by mincing and incubating with collagenase I 1 mg/ml (catalog LS004196; Worthington Biochemical), DNase I 5 mg/ml (D4263; Sigma), and RBC lysis buffer (catalog 50-112-9743;

(6) Bone regeneration using adipose derived stem cell spheroids within 3D printed scaffolds in a rabbit radial defect modelSci RepDecember 16, 2025Yangwon Chae, Kwangsik Jang, Sol Lee, Yong-Hun Kim, Songwan Jin, Kyung Mi Shim, Seong Soo Kang, Se Eun Kimwas chopped and digested for 1 h at 37 °C in a shaking water bath using 2 mg/ml collagenase type I (#LS004196, Worthington Biochemical Corporation, Worthington, NJ, USA) in Hank’s Balanced Salt Solution (HBSS,

(7) Daytime SHP2 inhibitor dosing, when immune cell numbers are elevated, shrinks neurofibromasLife Science AllianceDecember 1, 2025Niousha Ahmari, Kwangmin Choi, Jianqiang Wu, Tilat A Rizvi, Mark Jackson, Leah J Kershner, Mi-Ok Kim, Xiyuan Zhang, Eva Dombi, Jack Shern, et al.containing 20 ml L-15 medium (# 11415064; Thermo Fisher Scientific [Gibco]), 10 mg collagenase type I (LS004196; Worthington Biochemical), and 50 mg Dispase II (04942078001; Sigma-Aldrich) in a 37°C incubator for

(8) Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte differentiationSTAR ProtocolsDecember 1, 2025Qiqi Tang, Juan Xu, Xinyu Yang, Hanni Li, Lingdi Wang, Lu Zhu(penicillin-streptomycin),MCE,Cat# HY-K1006 75% ethanol,N/A,N/A Collagenase type 1,Worthington Biochemical,Cat# LS004196 Red blood cell lysis buffer,Solarbio,Cat# R1010 "CL-316,243",Sigma-Aldrich,Cat# C5976 Oil red O,Sigma-Aldrich,Cat#

(9) Adipose cullin 3 mediates the antiobesity effect of pan neddylation inhibitorsProceedings of the National Academy of SciencesOctober 28, 2025Lijie Gu, Yanhong Du, Mohammad Nazmul Nazmul Hasan, Yung-Dai Clayton, Tiangang Litissues were finely minced and subjected to enzymatic digestion using 1.25 mg/mL collagenase type I (LS004196, Worthington Biochemical Corporation, Lakewood, NJ) at 37 °C for 50 to 60 min. The enzymatic digestion

(10) White and brown adipose tissue share a convergent fibro-adipogenic progenitor populationEMBO ReportsOctober 8, 2025Hoang V Bui, Julia K Hansen, Valentina Lo Sardo, Andrea Galmozzipyruvate,Gibco,11360-070 GlutaMAX supplement,Gibco,35050-061 "Collagenase, Type 1",Worthington Biochemical,LS004196 Gelatin from porcine skin,Sigma-Aldrich,G1890 Trypsin-EDTA (0.05%),Gibco,25300-054 Trypan Blue,Sigma-Aldrich,T8154 Software,Software,Software GraphPad

(11) Transplantation of Cultured Myoblasts Into Intact Skeletal Muscle and Analysis of Muscle Contraction Force in Mice ModelBIO-PROTOCOLAugust 20, 2025Kitora Dohi, Yasuro FuruichiJapan SLC, Inc.) Reagents 1. Type 1 collagenase (Worthington Biochemical Corporation, catalog number: LS004196) 2. DMEM GlutaMAX (Gibco, catalog number: 10569044) 3. Penicillin-Streptomycin-Amphotericin B suspension

(12) The histone H3 lysine 36 demethylase KDM2A/FBXL11 controls Polycomb-mediated gene repression and germ cell development in male miceNat CommunJuly 23, 2025Michael T. Bocker, Grigorios Fanourgakis, Kristie Wetzel, Pavel A. Komarov, Hélène Royo, Alexia Rohmer, Sunwoo Chun, Ching-Yeu Liang, Hubertus Kohler, Taiping Chen, et al.prepared by incubation of seminiferous tubules in 200 U/ml Collagenase type I (Worthington Biochemical LS004196), 5 μg/ml DNAse I (Roche 10104159001), and 0.05%Trypsin (Gibco 25200056) in GBSS (Sigma G9779) as described

(13) Epitenon-derived progenitors drive fibrosis and regeneration after flexor tendon injury in a spatially-dependent mannerNat CommunJuly 1, 2025Anne E. C. Nichols, Lauren Benoodt, Emmanuella Adjei-Sowah, Kyle Jerreld, Alexander Kollar, Constantinos Ketonis, Alayna E. Loiselle11885084, Gibco, Thermo Fisher Scientific) on ice, and digested in DMEM containing Collagenase Type I (LS004196, Worthington Biochemical, Lakewood, NJ, USA) and Collagenase Type IV (LS004188, Worthington Biochemical)

(14) Protocols to co-culture human primary lung cells in the simple-flow deviceSTAR ProtocJune 17, 2025Cinta Iriondo, Sem Koornneef, Kari-Pekka Skarp, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Robbert J. Rottierbuffer solution,"Gibco, Thermo Fisher Scientific",15630–056 Collagenase type I,Worthington Biochemical,"LS004196, 1g" "Dispase I, neutral protease, grade I",Roche,04942086001 Collagen R solution (0.4%),Serva,47256.01 Glacial

(15) Lack of pathogenic involvement of CCL4 and its receptor CCR5 in arthritogenic alphavirus diseaseImmunohorizonsJune 4, 2025Muddassar Hameed, Norman A Solomon, James Weger-Lucarellithe ankle, deskinned, and transferred to digestion media [RPMI-10, 2.5 mg/ml Collagenase I (Cat. No. LS004196, Worthington Biochemical Corporation), 17 μg/ml DNase I (Cat. No. LS006333, Worthington Biochemical Corporation

(16) Harnessing cell size to separate genetically and functionally distinct dental pulp-derived mesenchymal stromal cell subpopulationsJ Biol EngJune 3, 2025Yiyue Jiang, Zheng Zhang, Kangkang Ren, Shujuan Zhou, Yang Qiao, Weilu Huang, Nanyang Zhang, Hanyan Xu, Xinping Xu, Bing Wen, et al.then subjected to enzymatic digestion with Collagenase I solution (2 mg/mL, Worthington Biochemical, LS004196) at 37 oC for 1 h. Following digestion, the resulting single cells from the dental pulp tissue were

(17) Protocol for pull-down capture of ubiquitinated and NEDDylated liver proteins from transgenic biotinylated ubiquitin and NEDD8 miceSTAR ProtocolsJune 1, 2025L. Estefanía Zapata-Pavas, Teresa C. Delgado, María Luz Martínez-ChantarCoulter,Allegra X-22 Cell scrapers,Corning,Cat# CLS3010 "Collagenase, type 1",Worthington Biochemical,Cat# LS004196 N-ethylmaleimide (NEM),Sigma-Aldrich,Cat# 04260 Protease inhibitor cocktail (PI),Sigma-Aldrich,Cat#

(18) Functional tendon regeneration is driven by regulatory T cells and IL-33 signalingScience advancesApril 25, 2025Arvind V, Crosio G, Howell K, Zhang H, Montero A, Huang AH.hours at 37°C on a rocker. Collagenase solution was prepared with collagenase I (5 mg/ml) (catalog no. LS004196, Worthington Biochemical) and collagenase IV (1 mg/ml) (catalog no. LS004188, Worthington Biochemical)

(19) Ogerin induced activation of Gpr68 alters tendon healingFASEB BioAdvancesApril 3, 2025Andrew Rodenhouse, Gilbert Smolyak, Emmanuela Adjei‐Sowah, Neeta Adhikari, Samantha Muscat, Takuma Okutani, Constantinos Ketonis, Anne E. C. Nichols, Robert M. Kottmann, Alayna E. LoiselleBriefly, tendons were digested in low glucose DMEM (#11885084, Gibco) containing Collagenase I (5 mg/mL; #LS004196, Worthington Biochemical, Lakewood, NJ, USA) and IV (1 mg/mL; #LS004188, Worthington Biochemical) for

(20) Adhesion strength of tumor cells predicts metastatic disease in vivoCell ReportsMarch 25, 2025Madison A. Kane, Katherine G. Birmingham, Benjamin Yeoman, Neal Patel, Hayley Sperinde, Thomas G. Molley, Pranjali Beri, Jeremy Tuler, Aditya Kumar, Sarah Klein, et al.97061-370 Rat Collagen I,Corning,Cat#354236 Collagenase Type I,Worthington Biochemical Corporation,Cat#LS004196 Critical commercial assays,Critical commercial assays,Critical commercial assays RNeasy Mini Kit,Qiagen,Cat#74104 Deposited

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Collagenase, Type 1 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 1 from Worthington Biochemical Corporation

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