Collagenase, Type 1 LS004196 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 1
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004196
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Tumor immune microenvironment reconstitution in patient-derived organoids enables therapy modeling for NSCLCCell Reports MethodsJune 15, 2026Enrique Podaza, Jared Capuano, Hui-Hsuan Kuo, Majd Al Assaad, Geoffrey Markowitz, M. Victoria Revuelta, John Nguyen, Adriana Irizarry, Hiranmayi Ravichandran, Sarah Ackermann, et al.U/ml Collagenase IV,Thermo Fisher Scientific,17104019 1mg/ml Collagenase I,Worthington Biochemical,LS004196 1X B27,Gibco,17504044 10% noggin conditioned media,In house,In house 10% R-spondin conditioned media,In

(2) Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunityJ Clin InvestJune 1, 2026Yuheng Liao, Hanze Wang, Hengxin Liu, Xi Chen, Renqiang Sun, Xie Li, Zhen Yang, Chenying Liu, Wei Wu, Ziqian He, et al.(0.2 mg/mL; Sigma-Aldrich, catalog DN25) and collagenase I (1 mg/mL; Worthington Biochemical, catalog LS004196) in cell culture incubator for 20 minutes, and filtered with 70 μm cell strainers (BD Biosciences, now

(3) Proteostasis sustains T cell differentiation potential and tumor-infiltrating lymphocyte functionCellMay 14, 2026Nicole E. Scharping, Xuezhen Ge, Maria Inês Matias, Fulin Jiang, Allison Cafferata, Maximilian Heeg, Alexander Monell, Giovanni Galletti, Kitty P. Cheung, Angelica Rock, et al.Calf Serum Iron Supplemented,R&D Systems,Cat#S11950H "Collagenase, Type 1",Worthington Biochemical,Cat# LS004196 "HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™",Fisher Scientific,Cat#BP310500 "L-Glutamine ≥99

(4) Valosin-Containing Protein Contributes to Plexiform Neurofibroma Formation and Represents a Novel Therapeutic TargetCellsMay 6, 2026Lalitha Gopalan, Youjin Na, Liang Hu, Ashley Hall, Mi-Ok Kim, Eva Dombi, Sara Szabo, Nancy Ratner, Gang Huang, Jianqiang Wu#10-045-CV) containing 0.5 mg/mL of collagenase type 1 (Worthington Biochemical, Lakewood, NJ, USA; #LS004196) and 2.5 mg/mL of Dispase protease type II (Roche, Indianapolis, IN, USA; #C756V28) at 37 °C for 3 h

(5) The Mitochondrial NAD Transporter SLC25A51 in Adipocytes Regulates Adipose Tissue Mitochondrial Function and Systemic Metabolism During AgingAging CellApril 21, 2026Daiki Kojima, Keisuke Yaku, Shotaro Kosugi, Ryunosuke Mitsuno, Kenji Kaneko, Yoshinaga Kawano, Akihito Hishikawa, Seiya Mizuno, Manami Katoh, Akiko Satoh, et al.Isolation Adipose tissue samples were incubated in Krebs Ringer buffer containing 1 mg/mL of collagenase type I (#LS004196; Worthington Biochemical, Lakewood, NJ) at 37°C for 45 min. After collagenase digestion, adipocytes

(6) Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon RepairJournal of Orthopaedic ResearchApril 7, 2026Varun Arvind, Elaine Nagahara, Hui Zhang, Peter Shyu, X. Edward Edward Guo, Alice H. HuangFlow cytometry Dissected tendons were incubated in 5 mg/mL Collagenase I (Worthington Biochemical, cat # LS004196) and I mg/mL Collagenase IV (Worthington Biochemical, cat # LS004188) for 4 hours at 37°C on a rocker.

(7) The heterogeneity of dermal mesenchymal cells reproduced in skin equivalents regulates barrier function and elasticityEMBO ReportsApril 1, 2026Shun Kimura, Sachiko Sekiya, Sawa Yamashiro, Tetsutaro Kikuchi, Masatoshi Haga, Tatsuya Shimizusolution,Kurabo,HK-3120 Trypsin-neutralizing solution,Kurabo,HK-3220 Collagenase type 1,Worthington Biochemical Corp,LS004196 Thrombin from bovine plasma,Sigma,T4648-1KU Software,Software,Software ImageJ FIJI,https://imagej.net/ij/, BellCurve

(8) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.digested with Collagenase Type I and Collagenase Type IV (Worthington Biochemical Corporation, cat. nos. LS004196 and LS004188) for 30 min at 37 °C with shaking at 125 rpm. Digestion was quenched with ice-cold PBS

(9) The uncoordinated‐5 homologue A is a key receptor in netrin‐ligand‐mediated fast‐twitch myotube formation in male micePhysiological ReportsFebruary 17, 2026Takahiro Maeno, Tomoki Ushijima, Koichi Ojima, Yohei Ogawa, Sayuki Hayashi, Hikaru Imakyure, Rika Osaki, Ryuki Oyama, Aoi Ogawa, Akimasa Takano, et al.Soleus and EDL muscles were dissected from mice and treated with 0.5% (w/v) type I collagenase (cat. no. LS004196, Worthington Biochemical, Freehold, NJ, USA) in Dulbecco's modified Eagle's medium (DMEM; cat. no. 0845816,

(10) The mechanosensitive channel TRPV4 inhibits pulmonary inflammation by limiting NF-κB signaling in alveolar macrophagesScience SignalingDecember 23, 2025Adam M. Boulton, Megan E. Grund, Yuxin Wang, Erica M. Orsini, Yan Liu, Susamma Abraham, Lisa M. Grove, Ryan Musich, Caitlin M. Snyder, Haley Ricci, et al.with cold PBS. Lung tissue was digested by mincing and incubating with collagenase I 1 mg/ml (catalog LS004196; Worthington Biochemical), DNase I 5 mg/ml (D4263; Sigma), and RBC lysis buffer (catalog 50-112-9743;

(11) Bone regeneration using adipose derived stem cell spheroids within 3D printed scaffolds in a rabbit radial defect modelSci RepDecember 16, 2025Yangwon Chae, Kwangsik Jang, Sol Lee, Yong-Hun Kim, Songwan Jin, Kyung Mi Shim, Seong Soo Kang, Se Eun Kimwas chopped and digested for 1 h at 37 °C in a shaking water bath using 2 mg/ml collagenase type I (#LS004196, Worthington Biochemical Corporation, Worthington, NJ, USA) in Hank’s Balanced Salt Solution (HBSS,

(12) Daytime SHP2 inhibitor dosing, when immune cell numbers are elevated, shrinks neurofibromasLife Science AllianceDecember 1, 2025Niousha Ahmari, Kwangmin Choi, Jianqiang Wu, Tilat A Rizvi, Mark Jackson, Leah J Kershner, Mi-Ok Kim, Xiyuan Zhang, Eva Dombi, Jack Shern, et al.containing 20 ml L-15 medium (# 11415064; Thermo Fisher Scientific [Gibco]), 10 mg collagenase type I (LS004196; Worthington Biochemical), and 50 mg Dispase II (04942078001; Sigma-Aldrich) in a 37°C incubator for

(13) Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte differentiationSTAR ProtocolsDecember 1, 2025Qiqi Tang, Juan Xu, Xinyu Yang, Hanni Li, Lingdi Wang, Lu Zhu(penicillin-streptomycin),MCE,Cat# HY-K1006 75% ethanol,N/A,N/A Collagenase type 1,Worthington Biochemical,Cat# LS004196 Red blood cell lysis buffer,Solarbio,Cat# R1010 "CL-316,243",Sigma-Aldrich,Cat# C5976 Oil red O,Sigma-Aldrich,Cat#

(14) Adipose cullin 3 mediates the antiobesity effect of pan neddylation inhibitorsProceedings of the National Academy of SciencesOctober 28, 2025Lijie Gu, Yanhong Du, Mohammad Nazmul Nazmul Hasan, Yung-Dai Clayton, Tiangang Litissues were finely minced and subjected to enzymatic digestion using 1.25 mg/mL collagenase type I (LS004196, Worthington Biochemical Corporation, Lakewood, NJ) at 37 °C for 50–60 minutes. The enzymatic digestion

(15) White and brown adipose tissue share a convergent fibro-adipogenic progenitor populationEMBO ReportsOctober 8, 2025Hoang V Bui, Julia K Hansen, Valentina Lo Sardo, Andrea Galmozzipyruvate,Gibco,11360-070 GlutaMAX supplement,Gibco,35050-061 "Collagenase, Type 1",Worthington Biochemical,LS004196 Gelatin from porcine skin,Sigma-Aldrich,G1890 Trypsin-EDTA (0.05%),Gibco,25300-054 Trypan Blue,Sigma-Aldrich,T8154 Software,Software,Software GraphPad

(16) Transplantation of Cultured Myoblasts Into Intact Skeletal Muscle and Analysis of Muscle Contraction Force in Mice ModelBio ProtocAugust 20, 2025Kitora Dohi, Yasuro FuruichiJapan SLC, Inc.) Reagents 1. Type 1 collagenase (Worthington Biochemical Corporation, catalog number: LS004196) 2. DMEM GlutaMAX (Gibco, catalog number: 10569044) 3. Penicillin-Streptomycin-Amphotericin B suspension

(17) Transplantation of Cultured Myoblasts Into Intact Skeletal Muscle and Analysis of Muscle Contraction Force in Mice ModelBIO-PROTOCOLAugust 20, 2025Kitora Dohi, Yasuro FuruichiJapan SLC, Inc.) Reagents 1. Type 1 collagenase (Worthington Biochemical Corporation, catalog number: LS004196) 2. DMEM GlutaMAX (Gibco, catalog number: 10569044) 3. Penicillin-Streptomycin-Amphotericin B suspension

(18) The histone H3 lysine 36 demethylase KDM2A/FBXL11 controls Polycomb-mediated gene repression and germ cell development in male miceNat CommunJuly 23, 2025Michael T. Bocker, Grigorios Fanourgakis, Kristie Wetzel, Pavel A. Komarov, Hélène Royo, Alexia Rohmer, Sunwoo Chun, Ching-Yeu Liang, Hubertus Kohler, Taiping Chen, et al.prepared by incubation of seminiferous tubules in 200 U/ml Collagenase type I (Worthington Biochemical LS004196), 5 μg/ml DNAse I (Roche 10104159001), and 0.05%Trypsin (Gibco 25200056) in GBSS (Sigma G9779) as described

(19) Epitenon-derived progenitors drive fibrosis and regeneration after flexor tendon injury in a spatially-dependent mannerNat CommunJuly 1, 2025Anne E. C. Nichols, Lauren Benoodt, Emmanuella Adjei-Sowah, Kyle Jerreld, Alexander Kollar, Constantinos Ketonis, Alayna E. Loiselle11885084, Gibco, Thermo Fisher Scientific) on ice, and digested in DMEM containing Collagenase Type I (LS004196, Worthington Biochemical, Lakewood, NJ, USA) and Collagenase Type IV (LS004188, Worthington Biochemical)

(20) Protocols to co-culture human primary lung cells in the simple-flow deviceSTAR ProtocJune 17, 2025Cinta Iriondo, Sem Koornneef, Kari-Pekka Skarp, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Robbert J. Rottierbuffer solution,"Gibco, Thermo Fisher Scientific",15630–056 Collagenase type I,Worthington Biochemical,"LS004196, 1g" "Dispase I, neutral protease, grade I",Roche,04942086001 Collagen R solution (0.4%),Serva,47256.01 Glacial

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Collagenase, Type 1 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 1 from Worthington Biochemical Corporation

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