Collagenase, Type 4 LS004188 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 4
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004188
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥160 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Poly-Pharmacologic Disruption of the Proliferative-to-Mesenchymal Fate Branch Point Reverses EndMT and Pulmonary HypertensionResearchSquareJuly 23, 2026Ruslan Rafikov, Tetiana Kolodiazhna, Tetiana Kolodiazhna, Mathews V Varghese, Takanori Varghese, Takanori Radhakrishnan, Sruthi Radhakrishnan, Joel Hinkle, Maki Niihori, Maki Rafikova, et al.enzymatically digested in a solution containing collagenase type IV (1 mg/mL; Worthington Biochemical, LS004188), neutral protease (1 mg/mL; Worthington Biochemical, LS02109), and DNase I (1 mg/mL; Roche, 82672500)

(2) Single-cell characterization of anterior segment development in the mouse reveals the cell types, pathways, and signals driving formation of the trabecular meshwork and Schlemm’s canalElifeJuly 21, 2026Revathi Balasubramanian, Nicholas Tolman, Taibo Li, Abdul Hannan, Violet Bupp-Chickering, Karina Polanco, Aakriti Bhandari, Sally Zhou, Marina Simón, John Peregrin, et al.,Thermo Fisher Scientific,Cat# S7020, "Chemical compound, drug",Collagenase Type 4,Worthington Biochemical,Cat# LS004188,1 mg/ml Other,DAPI,Thermo Fisher Scientific,Cat# 62248,(1:5000) Section ‘Immunohistochemistry’ Other,40

(3) Fluorescence tracking CD8 T cells from tumor-draining lymph nodes to irradiated tumors and their activation in the tumor environmentJ ImmunolJuly 17, 2026David Friedman, Gwen Kramer, Alejandro F Alice, Jason Baird, Sina Ramin, Terry Medler, Kristina H Young, Madeleine G Kaiser, Clemens Grassberger, Marka R Crittenden, et al.Miltenyi Biotec containing enzyme digest mix with 250 U/mL collagenase IV (Worthington Biochemical; #LS004188), 30U/mL DNase I (Millipore-Sigma; #4536282001), 5 mM CaCl2, 5% heat-inactivated fetal bovine serum

(4) Atg5 deficiency alters myofibroblast accumulation and alveolar regeneration in lung fibrosisStem Cell ReportsJuly 14, 2026Xue Li, Guiying Xu, Qi Wang, Qingwen Ma, Xue Liu, Hongwei Li, Sisi Wang, Yimeng Liu, Yueyue Jin, Xuan Miao, et al.,Worthington Biochemical Corporation,Cat#: LS002279 Collagenase Type 4,Worthington Biochemical Corporation,Cat#: LS004188 Dispase® II (neutral protease),Roche,Cat#: 04942078001 HiScript® Ⅲ RT SuperMix,Vazyme,Cat#: R323-01 SYBR

(5) Convection-Enhanced Delivery of Tumor-Infiltrating Lymphocytes Enhances Intratumoral Distribution and Therapeutic Efficacy in an Orthotopic Rat Glioma ModelBiomedicinesJune 28, 2026Yuan Zhou, Liwen Zhu, Xinglei Liu, Chunxia Ji, Jiakai Yao, Di Chen, Yu Yaoand enzymatically digested using collagenase IV (Worthington Biochemical, Lakewood, NJ, USA; Cat. No. LS004188) and DNase I (Grade II, Roche, Basel, Switzerland; Cat. No. 10104159001) at 37 °C for 30 min. After

(6) Circuit organization and transcriptomic heterogeneity of sympathetic circuits innervating cranial structuresCell ReportsJune 23, 2026Gokhan Senturk, Shawn Driscoll, Peter J. Osseward, Miriam Gullo, Karen Lettieri, Samuel L. Pfaff,Fisher Scientific,BP229-1 Bovine Serum Albumin (BSA),Sigma-Aldrich,A3059-50G Collagenase IV,Worthington,LS004188 Critical commercial assays,Critical commercial assays,Critical commercial assays Papain Dissociation System

(7) Tumor suppressor genotype influences the extent and mode of immunosurveillance in lung cancerNature CommunicationsJune 15, 2026Keren M. Adler, Haiqing Xu, Amy C. Gladstein, Valerie M. Irizarry-Negron, Maggie R. Robertson, Katherine R. Doerig, Dmitri A. Petrov, Monte M. Winslow, David M. Feldsermedia (-Mg2+ -Ca2+), 100 uL Trypsin-EDTA (0.25%), 100 uL Collagenase IV (Worthington Biochemical Corp., LS004188, from 10 mg/mL stock dissolved in HBSS), 0.5U Dispase (Corning, 354235, 5 U/mL stock). Samples were

(8) Single-cell RNA sequencing of terminal ileal biopsies identifies signatures of Crohn’s disease pathogenesisNature GeneticsJune 15, 2026Monika Krzak, Tobi Alegbe, D. Leland Taylor, Gareth-Rhys Jones, Mennatallah Ghouraba, Michelle Strickland, Bradley T. Harris, Reem Satti, Kenneth Arestang, Lucia Ramirez-Navarro, et al.in HBSS with Mg2+ and Ca2+ and without phenol red—including 5 mM CaCl2, 1.5 U µl−1 collagenase IV (LS004188; Worthington Biochemical) and 0.1 mg ml−1 DNase I (07900; Stem Cell Technologies). Next, 0.5 M EDTA

(9) Multiparametric Flow Cytometry Panel for Characterization of Mouse T Cell Differentiation and NK Cell Maturation Following Inflammatory ChallengeMethods ProtocJune 12, 2026Tim Bozic, Bostjan Markelc, Simona Kranjc Brezar, Ziva Pisljar, Tanja Jesenko, Maja Cemazaraeruginosa 10 (LPS) (Cat. N. L9143-10MG; Sigma-Aldrich, Darmstadt, Germany).Collagenase IV (1 g) (Cat. N. LS004188; Worthington Biochemical, Lakewood, NJ, USA).Hyaluronidase (100 mg) (Cat. N. H3506; Sigma-Aldrich, Darmstadt,

(10) Overexpression of small-conductance Ca2+-activated K+ channel 2 attenuates pain-like behavior in female mice with cystitisJCI InsightMay 22, 2026Guadalupe Manrique-Maldonado, Xuejiao Sun, Allison L. Marciszyn, Nicolas Montalbetti, Marcelo D. CarattinoNeurobasal-A Medium supplemented with 10 mg of collagenase type 4 (Worthington Biochemical, catalog LS004188) and 5 mg of trypsin (Worthington Biochemical, catalog LS003703). The cell culture dish flask containing

(11) Human epidermal growth factor receptor 3-targeted near-infrared photoimmunotherapy in a xenograft mouse model of breast cancerBMC CancerMay 18, 2026Makoto Kano, Aki Furusawa, Hiroshi Fukushima, Seiichiro Takao, Shuhei Okuyama, Hiroshi Yamamoto, Motofumi Suzuki, Ko Kitamura, Miyu Kano, Peter L Choyke, et al.100 mm³ in volume were excised and enzymatically dissociated using collagenase type IV (1 mg/mL, Cat# LS004188, Worthington Biochemical, Lakewood, NJ, USA) and DNase I (20 µg/mL, Cat# 10104159001, Millipore Sigma).

(12) Multicohort analysis unveils axon guidance pathways linking small for gestational age to spirometric restrictionNature CommunicationsMay 2, 2026James F. Read, Debra A. Stern, Tara F. Carr, Amber L. Spangenberg, Meiven Yang, Rosa I. Luna-Ramirez, Stefano Guerra, Wayne J. Morgan, Alex T. Binder, Jeffrey J. VanWormer, et al.G7021-100G], 10% FBS [Omega-Scientific, FB-11], 500U/ml Collagenase IV [Worthington Biochemical Corporation, LS004188] and 0.1 mg/ml DNase I [Sigma Aldrich, DN25]). Tissues in media were incubated for 30 min at 37 °C at

(13) Homeostatic mature dendritic cells instruct fibroblast specialization via Notch2 signaling to establish T cell nichesImmunityApril 23, 2026Nagham Alouche, Hélène Cannelle, Stéphanie Favre, Marija Jokić, José A. Villegas, Sandra Maréchal, Clint De Nolf, Maeva Delacrétaz, Alexandros Sifis, Chloé Chapuis, et al."Collagenase D, from Clostridium histolyticum",Roche,Cat#11088858001 "Type 4 collagenase, from Clostridium histolyticum",Worthington Biochemical,Cat#LS004188 Diphtheria toxin,Sigma Aldrich,Cat#D0564 Dapi,Sigma Aldrich,Cat#D9542 Tn5 transposase,Illumina,Cat#FC-121-1031 TD

(14) Astragalus Polysaccharides Induce Immunogenic Cell Death in Melanoma: A Mechanism Mediated by cGAS/STING Activation via Intratumoral Microbiota ModulationCancer MedApril 14, 2026Xi Qiao, Guiqing Ding, Qijin Lu, Yuxin Chen, Xiaohan Wang, Yuanhua Wang, Jinyun Ma, Xiaodong Chengrefer to Table 1. 2.7 Flow Cytometry Tumor tissues were dissociated with Collagenase Type IV 1 mg/mL (LS004188, Worthington Biochemical, USA), Neutral protease 1 mg/mL (88922N, Worthington Biochemical, China), and

(15) Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon RepairJournal of Orthopaedic ResearchApril 7, 2026Varun Arvind, Elaine Nagahara, Hui Zhang, Peter Shyu, X. Edward Edward Guo, Alice H. Huang(Worthington Biochemical, cat # LS004196) and I mg/mL Collagenase IV (Worthington Biochemical, cat # LS004188) for 4 hours at 37°C on a rocker. Isolated cells were then washed and resuspended in buffer (2% FBS

(16) RUNX2 enhances vascular remodeling to promote endothelial proliferation in acute liver injuryInt J Biol SciMarch 28, 2026Jong-Min Lee, Yoon-Su Ha, Seung-Jun Lee, Hyun-Yi Kim, Anish Ashok Adpaikar, Eun-Jung Kim, Keishi Otsu, Xiangguo Che, Dai Hoon Han, Young Her, et al.endothelial cell isolation Mouse livers were digested by retrograde perfusion with collagenase type 4 (LS004188, Worthington Biochemical Corporation, NJ, USA) through the portal vein. The dissociated cell mixture

(17) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.Collagenase Type I and Collagenase Type IV (Worthington Biochemical Corporation, cat. nos. LS004196 and LS004188) for 30 min at 37 °C with shaking at 125 rpm. Digestion was quenched with ice-cold PBS containing 2%

(18) Pain Outcome Determines the Sensitivity to Peripheral Opioid Antagonism of Morphine, Ibuprofen, and Their Combination in Laparotomized MicePharmaceuticsMarch 21, 2026Makeya A. Hasoun, Miriam Santos-Caballero, Miguel Á. Huerta, María Robles-Funes, Amada Puerto-Moya, M. Carmen Ruiz-Cantero, Enrique J. Cobos, Rafael González-Canoharvested samples underwent enzymatic dissociation in a solution containing 1 mg/mL collagenase IV (LS004188, Worthington, Lakewood, NJ, USA) and 0.1% DNase I (LS002007, Worthington Biochemical Corporation, Lakewood,

(19) CAPZA1 deficiency disrupts sperm flagellar structure and motility, potentially involving the p300/SLC7A11 pathwayFront Endocrinol (Lausanne)March 4, 2026Hui Lu, Guoxuan Li, Jiajia Hu, Hailing Ruan, Liqiang Zhao, Yejuan Li, Anguo Wang14060-09). Tissue fragments were digested with 0.5 mg/mL of collagenase IV (Worthington Biochemical, LS004188) at 37 °C for 10 min in a shaking incubator (Thermo Fisher Scientific, MaxQ 4450), followed by 2 mL

(20) GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic AdenocarcinomaCancer ResearchMarch 2, 2026Ziheng Chen, I-Lin Ho, Jintan Liu, Melinda Soeung, Er-Yen Yen, Sara Sainani, Charles A. Dyke, Rutvi Shah, Hyuk Jean Kwon, Guangchun Han, et al.containing DNase I (Roche Diagnostics #4536282001) and 2 mg/mL collagenase type IV (Worthington Biochemical #LS004188) 40 minutes at 37°C under agitation. The digestion buffer was then washed out with PBS, followed by

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Collagenase, Type 4 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 4 from Worthington Biochemical Corporation

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