Collagenase, Type 4 LS004188 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 4
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004188
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥160 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Astragalus Polysaccharides Induce Immunogenic Cell Death in Melanoma: A Mechanism Mediated by cGAS/STING Activation via Intratumoral Microbiota ModulationCancer MedApril 14, 2026Xi Qiao, Guiqing Ding, Qijin Lu, Yuxin Chen, Xiaohan Wang, Yuanhua Wang, Jinyun Ma, Xiaodong Chengrefer to Table 1. 2.7 Flow Cytometry Tumor tissues were dissociated with Collagenase Type IV 1 mg/mL (LS004188, Worthington Biochemical, USA), Neutral protease 1 mg/mL (88922N, Worthington Biochemical, China), and

(2) Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon RepairJournal of Orthopaedic ResearchApril 7, 2026Varun Arvind, Elaine Nagahara, Hui Zhang, Peter Shyu, X. Edward Edward Guo, Alice H. Huang(Worthington Biochemical, cat # LS004196) and I mg/mL Collagenase IV (Worthington Biochemical, cat # LS004188) for 4 hours at 37°C on a rocker. Isolated cells were then washed and resuspended in buffer (2% FBS

(3) RUNX2 enhances vascular remodeling to promote endothelial proliferation in acute liver injuryInt J Biol SciMarch 28, 2026Jong-Min Lee, Yoon-Su Ha, Seung-Jun Lee, Hyun-Yi Kim, Anish Ashok Adpaikar, Eun-Jung Kim, Keishi Otsu, Xiangguo Che, Dai Hoon Han, Young Her, et al.endothelial cell isolation Mouse livers were digested by retrograde perfusion with collagenase type 4 (LS004188, Worthington Biochemical Corporation, NJ, USA) through the portal vein. The dissociated cell mixture

(4) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.Collagenase Type I and Collagenase Type IV (Worthington Biochemical Corporation, cat. nos. LS004196 and LS004188) for 30 min at 37 °C with shaking at 125 rpm. Digestion was quenched with ice-cold PBS containing 2%

(5) Pain Outcome Determines the Sensitivity to Peripheral Opioid Antagonism of Morphine, Ibuprofen, and Their Combination in Laparotomized MicePharmaceuticsMarch 21, 2026Makeya A. Hasoun, Miriam Santos-Caballero, Miguel Á. Huerta, María Robles-Funes, Amada Puerto-Moya, M. Carmen Ruiz-Cantero, Enrique J. Cobos, Rafael González-Canoharvested samples underwent enzymatic dissociation in a solution containing 1 mg/mL collagenase IV (LS004188, Worthington, Lakewood, NJ, USA) and 0.1% DNase I (LS002007, Worthington Biochemical Corporation, Lakewood,

(6) CAPZA1 deficiency disrupts sperm flagellar structure and motility, potentially involving the p300/SLC7A11 pathwayFront Endocrinol (Lausanne)March 4, 2026Hui Lu, Guoxuan Li, Jiajia Hu, Hailing Ruan, Liqiang Zhao, Yejuan Li, Anguo Wang14060-09). Tissue fragments were digested with 0.5 mg/mL of collagenase IV (Worthington Biochemical, LS004188) at 37 °C for 10 min in a shaking incubator (Thermo Fisher Scientific, MaxQ 4450), followed by 2 mL

(7) GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic AdenocarcinomaCancer ResearchMarch 2, 2026Ziheng Chen, I-Lin Ho, Jintan Liu, Melinda Soeung, Er-Yen Yen, Sara Sainani, Charles A. Dyke, Rutvi Shah, Hyuk Jean Kwon, Guangchun Han, et al.containing DNase I (Roche Diagnostics #4536282001) and 2 mg/mL collagenase type IV (Worthington Biochemical #LS004188) 40 minutes at 37°C under agitation. The digestion buffer was then washed out with PBS, followed by

(8) CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assemblyNatureFebruary 25, 2026Alyssa J. Mathiowetz, Emily S. Meymand, Güneş Parlakgül, Niek van Hilten, Emily F. Torres, Leonardo L. Artico, Kirandeep K. Deol, Mike Lange, Stephany P. Pang, Cody E. Doubravsky, et al.NaHCO3, BSA (fatty acid-free) 7.2 mg ml−1 and 0.18 mg ml−1 type IV collagenase (Worthington Biochemical, LS004188)), BSA and collagenase were added immediately before use. The buffers were kept at ~37 °C during the

(9) Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combinationiScienceFebruary 20, 2026David J. Friedman, Sina Ramin, Tiffany Blair, Gwen Kramer, Shelly Bambina, Alejandro F. Alice, Jason Baird, Terry Medler, Kristina H. Young, Marka R. Crittenden, et al.containing enzyme digestion mixture, consisting of with 250 U/ml collagenase IV (Worthington Biochemical, LS004188), 30 U/ml DNase I (MilliporeSigma, 453628200), 5 mM CaCl2, 5% heat-inactivated FBS (Gibco, 16000-069),

(10) Chimeric MHC class I- and II-restricted non-self epitopes broaden antitumor T cell reactionsJ Exp MedFebruary 2, 2026Rongsheng Zhang, Rong Ma, Merrin M.L. Leong, Ian R. Watson, Kei Iida, Tomonori Yaguchi, Fumihiko Matsuda, Tasuku Honjo, Kenji Chamotoand cut into 1–2-mm pieces with scissors, followed by digestion with collagenase type IV (catalog #LS004188; Worthington Biochemical Corporation) using a gentle MACS Dissociator (Miltenyi Biotec). Establishment

(11) Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatmenteLifeJanuary 20, 2026Nicholas Tolman, Taibo Li, Revathi Balasubramanian, Guorong Li, Rebecca Pfeiffer, Violet Bupp-Chickering, Ruth A Kelly, Marina Simón, John Peregrin, Christa Montgomery, et al.University) Single cell dissociation was performed using Collagenase IV (Worthington Biochemical Cat. no. LS004188), Dispase II (Sigma Cat. No. D4693) and DNAse I (Roche Cat no. 50225500) for 60 min and then trypsin/EDTA

(12) Daytime SHP2 inhibitor dosing, when immune cell numbers are elevated, shrinks neurofibromasLife Science AllianceDecember 1, 2025Niousha Ahmari, Kwangmin Choi, Jianqiang Wu, Tilat A Rizvi, Mark Jackson, Leah J Kershner, Mi-Ok Kim, Xiyuan Zhang, Eva Dombi, Jack Shern, et al.collagenase A (100 mg/ml) (# 10103586001; Sigma-Aldrich), 100 μl collagenase type 4 (100 mg/ml) (# LS004188; Worthington Biochemical), 40 μl soybean trypsin inhibitor (100 μg/ml) (# T9128; Sigma-Aldrich), 10

(13) Protocol for generation of immortalized retinal, choroid, and brain endothelial cell linesSTAR ProtocolsDecember 1, 2025Laura Hannig, Jakob S. Bernhard, Wolfgang Lezuo, Robin Heiden, Mario Vallon, Süleyman Ergün, Carola Y. Förster, Anja Schlecht, Barbara M. Braunger(Life Technologies),15630080 Ampuwa,Fresenius,1080181 Bovine serum albumin (BSA),Merck KGaA (Millipore),81-053-3 Collagenase IV,Worthington Biochemical,LS004188 DNase I,Worthington Biochemical,LS006342 Dulbecco’s modified Eagle’s medium (DMEM),Thermo Fisher Scientific,41965-039 Fetal

(14) A combined genomic arrhythmia propensity score delineates cumulative riskCell Reports MedicineNovember 18, 2025Tanner O. Monroe, Megan J. Puckelwartz, Lorenzo L. Pesce, Samuel D. Kearns, Patrick Page, Nora Ibrahim, Zachary T. Weber, Emmanuel I. Ugwor, Marcelo A. Nóbrega, Prince Kannankeril, et al.dissociated to single cells using 200units/mL collagenase IV (Worthington Biochemical Corporation cat# LS004188) in Hank’s Balanced Salt Solution (HBSS cat# 14025092, Thermo with 10mM HEPES, 2μM Thiazovivin (TZV)

(15) Multiomic and electrophysiologic analyses reveal that an inherited MRC2 variant causes fibroblast dysfunction and increased atrial fibrillation susceptibilityAmerican Journal of Physiology-Heart and Circulatory PhysiologyNovember 1, 2025Kevin S. Ho, Joshua A. Keefe, Shuai Zhao, Mohit M. Hulsurkar, Sung Yun Jung, Md. Abul Hassan Samee, Xander H. T. Wehrenswere excised and placed in dissociation solution containing 1 mg (280 U) per mL collagenase, type 4 (LS004188, Worthington Biochemical Corp., Lakewood, NJ), and 0.7 mg (1 U) per mL dispase II (D4693, Sigma-Aldrich,

(16) Regenerative Potential of Neonatal Tendons Is Regulated by Intrinsic Resistance to Inflammation and Supportive T‐Cell–Secreted FactorsThe FASEB JournalOctober 31, 2025Giulia Crosio, Emily R. King, Alice H. Huangcentrifuge tubes with 1mg/ml collagenase IV and 5mg/ml collagenase I (Worthington Biochemical, cat: LS004188 andLS004196, respectively) in serum-free Dulbecco’s Modified Eagle Medium (Fisher, ref: 11965–092) with

(17) The immunological paradox of tubal ectopic pregnancy: unraveling immune tolerance amidst inflammation via single-cell sequencingBMC Pregnancy ChildbirthOctober 8, 2025Suming Xu, Ningxin Zhang, Yaoqin Wang, Xingyu Bi, Yonglian Wang, Xianping Wang, Pengfei Zhu, Xueqing Wuthen incubated in a dissociation solution containing 0.35% Collagenase IV (Worthington Biochemical, LS004188) and 120 U/mL DNase I (Roche, 11284932001) at 37℃ in a water bath shaker (100 rpm) for ~ 20 min. The

(18) Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatmentbioRxivSeptember 25, 2025Nicholas Tolman, Taibo Li, Revathi Balasubramanian, Guorong Li, Rebecca Pfeiffer, Violet Bupp-Chickering, Ruth A. Kelly, Marina Simón, John Peregrin, Christa Montgomery, et al.University): Single cell dissociation was performed using Collagenase IV (Worthington Biochemical Cat. no. LS004188), Dispase II (Sigma Cat. No. D4693) and DNAse I (Roche Cat no. 50225500) for 60 min and then trypsin/EDTA

(19) Chronic Waterborne Exposure to Polystyrene Microplastics Induces Kupffer Cell Polarization Imbalance and Hepatic Lipid AccumulationThe FASEB JournalSeptember 15, 2025Ting Li, Zhenghong Yao, Yuxin Huang, Ran Li, Lu Zhang, Rucheng Chen, Weijia GuDevelopment Center (Tianjin, China). Other reagents included DMEM (#8123594, Gibco, China), collagenase (#LS004188, Worthington Biochemical Corporation, USA), Deoxyribonuclease I (#LS002138, Worthington Biochemical Corporation

(20) Porphyrin-based nanotechnology: a minimally invasive approach for drug delivery and cholesteatoma treatmentFrontiers in SurgerySeptember 3, 2025Dina Ashraf Mahmoud, Lili Ding, Zannatul Ferdous, Zhifen Zhang, Juan Chen, Jennifer L. Spiegel, Edward Alexander Sykes, Robert Harrison, Gang Zheng, Trung Lewere then carefully diced and put in 2 ml Eppendorf of 200 U/ml collagenase IV in DMEM/F12 (Cat. No. LS004188, Worthington Biochemical Corporation, 730 Vassar Ave, NJ 08701, USA) overnight at 4 ℃, employing a shaker

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Collagenase, Type 4 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 4 from Worthington Biochemical Corporation

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