Collagenase, Type 4 LS004188 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 4
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004188
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥160 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Multiparametric Flow Cytometry Panel for Characterization of Mouse T Cell Differentiation and NK Cell Maturation Following Inflammatory ChallengeMethods ProtocJune 12, 2026Tim Bozic, Bostjan Markelc, Simona Kranjc Brezar, Ziva Pisljar, Tanja Jesenko, Maja Cemazaraeruginosa 10 (LPS) (Cat. N. L9143-10MG; Sigma-Aldrich, Darmstadt, Germany).Collagenase IV (1 g) (Cat. N. LS004188; Worthington Biochemical, Lakewood, NJ, USA).Hyaluronidase (100 mg) (Cat. N. H3506; Sigma-Aldrich, Darmstadt,

(2) Overexpression of small-conductance Ca2+-activated K+ channel 2 attenuates pain-like behavior in female mice with cystitisJCI InsightMay 22, 2026Guadalupe Manrique-Maldonado, Xuejiao Sun, Allison L. Marciszyn, Nicolas Montalbetti, Marcelo D. CarattinoNeurobasal-A Medium supplemented with 10 mg of collagenase type 4 (Worthington Biochemical, catalog LS004188) and 5 mg of trypsin (Worthington Biochemical, catalog LS003703). The cell culture dish flask containing

(3) Homeostatic mature dendritic cells instruct fibroblast specialization via Notch2 signaling to establish T cell nichesImmunityApril 23, 2026Nagham Alouche, Hélène Cannelle, Stéphanie Favre, Marija Jokić, José A. Villegas, Sandra Maréchal, Clint De Nolf, Maeva Delacrétaz, Alexandros Sifis, Chloé Chapuis, et al."Collagenase D, from Clostridium histolyticum",Roche,Cat#11088858001 "Type 4 collagenase, from Clostridium histolyticum",Worthington Biochemical,Cat#LS004188 Diphtheria toxin,Sigma Aldrich,Cat#D0564 Dapi,Sigma Aldrich,Cat#D9542 Tn5 transposase,Illumina,Cat#FC-121-1031 TD

(4) Astragalus Polysaccharides Induce Immunogenic Cell Death in Melanoma: A Mechanism Mediated by cGAS/STING Activation via Intratumoral Microbiota ModulationCancer MedApril 14, 2026Xi Qiao, Guiqing Ding, Qijin Lu, Yuxin Chen, Xiaohan Wang, Yuanhua Wang, Jinyun Ma, Xiaodong Chengrefer to Table 1. 2.7 Flow Cytometry Tumor tissues were dissociated with Collagenase Type IV 1 mg/mL (LS004188, Worthington Biochemical, USA), Neutral protease 1 mg/mL (88922N, Worthington Biochemical, China), and

(5) Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon RepairJournal of Orthopaedic ResearchApril 7, 2026Varun Arvind, Elaine Nagahara, Hui Zhang, Peter Shyu, X. Edward Edward Guo, Alice H. Huang(Worthington Biochemical, cat # LS004196) and I mg/mL Collagenase IV (Worthington Biochemical, cat # LS004188) for 4 hours at 37°C on a rocker. Isolated cells were then washed and resuspended in buffer (2% FBS

(6) RUNX2 enhances vascular remodeling to promote endothelial proliferation in acute liver injuryInt J Biol SciMarch 28, 2026Jong-Min Lee, Yoon-Su Ha, Seung-Jun Lee, Hyun-Yi Kim, Anish Ashok Adpaikar, Eun-Jung Kim, Keishi Otsu, Xiangguo Che, Dai Hoon Han, Young Her, et al.endothelial cell isolation Mouse livers were digested by retrograde perfusion with collagenase type 4 (LS004188, Worthington Biochemical Corporation, NJ, USA) through the portal vein. The dissociated cell mixture

(7) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.Collagenase Type I and Collagenase Type IV (Worthington Biochemical Corporation, cat. nos. LS004196 and LS004188) for 30 min at 37 °C with shaking at 125 rpm. Digestion was quenched with ice-cold PBS containing 2%

(8) Pain Outcome Determines the Sensitivity to Peripheral Opioid Antagonism of Morphine, Ibuprofen, and Their Combination in Laparotomized MicePharmaceuticsMarch 21, 2026Makeya A. Hasoun, Miriam Santos-Caballero, Miguel Á. Huerta, María Robles-Funes, Amada Puerto-Moya, M. Carmen Ruiz-Cantero, Enrique J. Cobos, Rafael González-Canoharvested samples underwent enzymatic dissociation in a solution containing 1 mg/mL collagenase IV (LS004188, Worthington, Lakewood, NJ, USA) and 0.1% DNase I (LS002007, Worthington Biochemical Corporation, Lakewood,

(9) CAPZA1 deficiency disrupts sperm flagellar structure and motility, potentially involving the p300/SLC7A11 pathwayFront Endocrinol (Lausanne)March 4, 2026Hui Lu, Guoxuan Li, Jiajia Hu, Hailing Ruan, Liqiang Zhao, Yejuan Li, Anguo Wang14060-09). Tissue fragments were digested with 0.5 mg/mL of collagenase IV (Worthington Biochemical, LS004188) at 37 °C for 10 min in a shaking incubator (Thermo Fisher Scientific, MaxQ 4450), followed by 2 mL

(10) GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic AdenocarcinomaCancer ResearchMarch 2, 2026Ziheng Chen, I-Lin Ho, Jintan Liu, Melinda Soeung, Er-Yen Yen, Sara Sainani, Charles A. Dyke, Rutvi Shah, Hyuk Jean Kwon, Guangchun Han, et al.containing DNase I (Roche Diagnostics #4536282001) and 2 mg/mL collagenase type IV (Worthington Biochemical #LS004188) 40 minutes at 37°C under agitation. The digestion buffer was then washed out with PBS, followed by

(11) Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polaritybioRxivFebruary 28, 2026Keith A. Breau, Emma G. Dunahey, Henry V. Fosnocht, Scott T. Magness, Timothy C. Elstonwith collagenase IV (750 U/mL final concentration) at 37 C for 20 minutes (Worthington Biochemical #LS004188). The resulting cells were rinsed with PBS, incubated in warm TrypLE Express (ThermoFisher Scientific

(12) CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assemblyNatureFebruary 25, 2026Alyssa J. Mathiowetz, Emily S. Meymand, Güneş Parlakgül, Niek van Hilten, Emily F. Torres, Leonardo L. Artico, Kirandeep K. Deol, Mike Lange, Stephany P. Pang, Cody E. Doubravsky, et al.NaHCO3, BSA (fatty acid-free) 7.2 mg ml−1 and 0.18 mg ml−1 type IV collagenase (Worthington Biochemical, LS004188)), BSA and collagenase were added immediately before use. The buffers were kept at ~37 °C during the

(13) Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combinationiScienceFebruary 20, 2026David J. Friedman, Sina Ramin, Tiffany Blair, Gwen Kramer, Shelly Bambina, Alejandro F. Alice, Jason Baird, Terry Medler, Kristina H. Young, Marka R. Crittenden, et al.containing enzyme digestion mixture, consisting of with 250 U/ml collagenase IV (Worthington Biochemical, LS004188), 30 U/ml DNase I (MilliporeSigma, 453628200), 5 mM CaCl2, 5% heat-inactivated FBS (Gibco, 16000-069),

(14) Chimeric MHC class I- and II-restricted non-self epitopes broaden antitumor T cell reactionsJ Exp MedFebruary 2, 2026Rongsheng Zhang, Rong Ma, Merrin M.L. Leong, Ian R. Watson, Kei Iida, Tomonori Yaguchi, Fumihiko Matsuda, Tasuku Honjo, Kenji Chamotoand cut into 1–2-mm pieces with scissors, followed by digestion with collagenase type IV (catalog #LS004188; Worthington Biochemical Corporation) using a gentle MACS Dissociator (Miltenyi Biotec). Establishment

(15) Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatmenteLifeJanuary 20, 2026Nicholas Tolman, Taibo Li, Revathi Balasubramanian, Guorong Li, Rebecca Pfeiffer, Violet Bupp-Chickering, Ruth A Kelly, Marina Simón, John Peregrin, Christa Montgomery, et al.University) Single cell dissociation was performed using Collagenase IV (Worthington Biochemical Cat. no. LS004188), Dispase II (Sigma Cat. No. D4693) and DNAse I (Roche Cat no. 50225500) for 60 min and then trypsin/EDTA

(16) Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatmentElifeJanuary 20, 2026Nicholas Tolman, Taibo Li, Revathi Balasubramanian, Guorong Li, Rebecca Pfeiffer, Violet Bupp-Chickering, Ruth A Kelly, Marina Simón, John Peregrin, Christa Montgomery, et al.University) Single cell dissociation was performed using Collagenase IV (Worthington Biochemical Cat. no. LS004188), Dispase II (Sigma Cat. No. D4693) and DNAse I (Roche Cat no. 50225500) for 60 min and then trypsin/EDTA

(17) GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic AdenocarcinomaCancer ResDecember 2, 2025Ziheng Chen, I-Lin Ho, Jintan Liu, Melinda Soeung, Er-Yen Yen, Sara Sainani, Charles A. Dyke, Rutvi Shah, Hyuk Jean Kwon, Guangchun Han, et al.containing DNase I (Roche Diagnostics #4536282001) and 2 mg/mL collagenase type IV (Worthington Biochemical #LS004188) 40 minutes at 37°C under agitation. The digestion buffer was then washed out with PBS, followed by

(18) Protocol for generation of immortalized retinal, choroid, and brain endothelial cell linesSTAR ProtocolsDecember 1, 2025Laura Hannig, Jakob S. Bernhard, Wolfgang Lezuo, Robin Heiden, Mario Vallon, Süleyman Ergün, Carola Y. Förster, Anja Schlecht, Barbara M. Braunger(Life Technologies),15630080 Ampuwa,Fresenius,1080181 Bovine serum albumin (BSA),Merck KGaA (Millipore),81-053-3 Collagenase IV,Worthington Biochemical,LS004188 DNase I,Worthington Biochemical,LS006342 Dulbecco’s modified Eagle’s medium (DMEM),Thermo Fisher Scientific,41965-039 Fetal

(19) Daytime SHP2 inhibitor dosing, when immune cell numbers are elevated, shrinks neurofibromasLife Science AllianceDecember 1, 2025Niousha Ahmari, Kwangmin Choi, Jianqiang Wu, Tilat A Rizvi, Mark Jackson, Leah J Kershner, Mi-Ok Kim, Xiyuan Zhang, Eva Dombi, Jack Shern, et al.collagenase A (100 mg/ml) (# 10103586001; Sigma-Aldrich), 100 μl collagenase type 4 (100 mg/ml) (# LS004188; Worthington Biochemical), 40 μl soybean trypsin inhibitor (100 μg/ml) (# T9128; Sigma-Aldrich), 10

(20) A combined genomic arrhythmia propensity score delineates cumulative riskCell Reports MedicineNovember 18, 2025Tanner O. Monroe, Megan J. Puckelwartz, Lorenzo L. Pesce, Samuel D. Kearns, Patrick Page, Nora Ibrahim, Zachary T. Weber, Emmanuel I. Ugwor, Marcelo A. Nóbrega, Prince Kannankeril, et al.dissociated to single cells using 200units/mL collagenase IV (Worthington Biochemical Corporation cat# LS004188) in Hank’s Balanced Salt Solution (HBSS cat# 14025092, Thermo with 10mM HEPES, 2μM Thiazovivin (TZV)

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Collagenase, Type 4 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 4 from Worthington Biochemical Corporation

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