PureLink RNase A (20 mg/mL) 12091-021 from Thermo Fisher Scientific

Product Specs
ItemPureLink RNase A (20 mg/mL)
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog Number12091-021
FormatLiquid
Brand/TrademarkInvitrogen™
Quantity10 mL
Concentration20 mg/mL

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Carmofur prevents cell cycle progression by reducing E2F8 transcription in temozolomide-resistant glioblastoma cellsCell Death DiscovDecember 12, 2023Cyntanna C. Hawkins, Amber B. Jones, Emily R. Gordon, Yuvika Harsh, Julia K. Ziebro, Christopher D. Willey, Corinne Griguer, David K. Crossman, Sara J. Cooper, Sasanka Ramanadham, et al.UK, cat #ab14083) in the presence of PureLink RNase A at 1 mg/mL (Invitrogen, Waltham, MA, USA cat# 12091-021) for one hour before analyzing using flow cytometry. FACSymphony A5 Cell Analyzer (BD Biosciences, San

(2) Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formationNature Cardiovascular ResearchDecember 11, 2023Bianca de Jonckheere, Ferdinand Kollotzek, Patrick Münzer, Vanessa Göb, Melina Fischer, Kristina Mott, Cristina Coman, Nina Nicole Troppmair, Mailin-Christin Manke, Monika Zdanyte, et al.(Cell signaling 5872S), FcR blocking reagent mouse (Miltenyi Biotec 130-092-575), PureLink RNase A (Invitrogen 12091-021), propidium iodide (Invitrogen P1304MP), EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific 11851185),

(3) Neuronal-specific methylome and hydroxymethylome analysis reveal replicated and novel loci associated with alcohol use disordermedRxivNovember 30, 2023Diego Andrade-Brito, Diana L Nunez-Rios, Jose Jaime Martinez-Magana, Sheila T Nagamatsu, Gregory Rompala, Lea Zillich, Stephanie Witt, Shaunna L Clark, Maria C Lattig Matiz, Janitza L. Montalvo-Ortiz, et al.Next, 500 μL and 50 μL proteinase K (Cat. #69504, Qiagen, Valencia, CA) and 20 mg/mL RNAse A (Cat. #12091021; Thermo-Fischer, Waltham, MA) were used to refloat the pellet. TheDNeasy Blood and Tissue Kit (Cat.

(4) Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish GonadsInternational Journal of Molecular SciencesNovember 6, 2023Alejandro Valdivieso, Marta Caballero-Huertas, Javier Moraleda-Prados, Francesc Piferrer, Laia Ribasstandard phenol–chloroform–isoamyl alcohol protocol (PCI 25:24:1, v/v/v) with 0.5 μg ribonuclease A (12091021, PureLink RNase A, Life Technologies, Carlsbad, CA, USA) was implemented to eliminate RNA traces. Five

(5) Liquid biopsy epigenomic profiling for cancer subtypingNature MedicineNovember 1, 2023Sylvan C. Baca, Ji-Heui Seo, Matthew P. Davidsohn, Brad Fortunato, Karl Semaan, Shahabbedin Sotudian, Gitanjali Lakshminarayanan, Miklos Diossy, Xintao Qiu, Talal El Zarif, et al.SDS and 0.6 mg ml−1 Proteinase K (Qiagen, 19131) and 0.4 mg ml−1 RNaseA (Thermo Fisher Scientific, 12091021) for 10 min at 37 °C, for 1 h at 50 °C and for 90 min at 65 °C. DNA was purified through phenol extraction

(6) Profiling neuronal methylome and hydroxymethylome of opioid use disorder in the human orbitofrontal cortexNature CommunicationsJuly 28, 2023Gregory Rompala, Sheila T. Nagamatsu, José Jaime Martínez-Magaña, Diana L. Nuñez-Ríos, Jiawei Wang, Matthew J. Girgenti, John H. Krystal, Joel Gelernter, Victor E. Alvarez, David Benedek, et al.down to 500 µL and 50 µL proteinase K (Cat. #69504, Qiagen, Valencia, CA) and 20 mg/mL RNAse A (Cat. #12091021; Thermo-Fischer, Waltham, MA) were added. The samples were processed following the manufacturer’s protocol

(7) Targeting Extracellular RNA Mitigates Hepatic Lipotoxicity and Liver Injury in NASHCellsJuly 24, 2023Archana Tewari, Sangam Rajak, Sana Raza, Pratima Gupta, Bandana Chakravarti, Jyotika Srivastava, Chandra P. Chaturvedi, Rohit A. Sinhawas continued up to 24 h to maintain a sustained release of RNase 1 (Invitrogen, Waltham, MA, USA, #12091021) in the culture medium. 2.2. MTT Assay HepG2 and AML12 cells (1 × 104), seeded into 96-well plates,

(8) Chromatin-RNA in situ Reverse Transcription Sequencing (CRIST-seq) Approach to Profile the Non-coding RNA Interaction NetworkBIO-PROTOCOLJuly 20, 2023shilin zhang, Xue Wen, Lei zhou, Hui Li, Wei Li, Andrew R. Hoffman, Ji-Fan Hu, Jiuwei Cuimagnetic beads (Millipore, catalog number: LSKMAGAG10)RNase A (20 mg/mL) (Invitrogen, catalog number: 12091021)AMPure XP beads (Beckman Coulter, catalog number: A63881)NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina

(9) Ubx5‐Cdc48 assists the protease Wss1 at DNA‐protein crosslink sites in yeastThe EMBO JournalJuly 3, 2023Audrey Noireterre, Nataliia Serbyn, Ivona Bagdiul, Françoise Stutz3,500 g for 10 min was performed, and cells were resuspended in 250 μl NaCi +5 μl RNase A (Invitrogen, 12091‐021) and incubated for 1 h at 37°C. Staining with 25 μg/ml Propidium Iodide (PI, Sigma, P4170) was performed

(10) Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in miceOncology reportsJuly 1, 2023Kim S, Song YK, Cho CS, Kim HJ, Fang S, Jo DH, Kim H.48 h and harvested. The harvested cells were washed and fixed. Next, 200 µl RNase (1 mg/ml; cat. no. 12091021; Thermo Fisher Scientific, Inc.) was added. The samples were incubated at 37°C for 30 min and stained

(11) Selective Killing of BRCA2-Deficient Ovarian Cancer Cells via MRE11 BlockadeInternational Journal of Molecular SciencesJune 30, 2023Adel Alblihy, Reem Ali, Mashael Algethami, Alison A. Ritchie, Ahmed Shoqafi, Shatha Alqahtani, Katia A. Mesquita, Michael S. Toss, Paloma Ordóñez-Morán, Jennie N. Jeyapalan, et al.Millipore, Watford, UK). For the cell cycle analysis, the cells were treated with 20 mg/mL of RNase A (12091021, Invitrogen), and then 10 mg/mL of Propidium Iodide (P4170, Sigma Aldrich, Gillingham, UK) was added

(12) Dependency of Tamoxifen Sensitive and Resistant ER+ Breast Cancer Cells on Semaphorin 3C (SEMA3C) for GrowthCellsJune 25, 2023Satyam Bhasin, Christopher Dusek, James W. Peacock, Artem Cherkasov, Yuzhuo Wang, Martin Gleave, Christopher J. OngSigma-Aldrich, Oakville, ON, Canada), Charcoal Strip Serum (12676029; Gibco, Grand Island, NY, USA), RNase A (12091021; Invitrogen, Carlsbad, CA, USA), Propidium Iodide (P3566; Invitrogen, Carlsbad, CA, USA) and Insulin-transferrin-selenium

(13) Characterization of a novel genus of jumbo phages and their application in wastewater treatmentiScienceJune 16, 2023Ming Hu, Bo Xing, Minghua Yang, Rui Han, Huazheng Pan, Hui Guo, Zhen Liu, Tao Huang, Kang Du, Shangyi Jiang, et al.recombinant proteins" ,, PEG8000,SIGMA,P5413-2KG DNase I,SIGMA,Cat # DN25 RNase A,Invitrogen,Cat # 12091021 Proteinase K,NEB,P8107S LB Broth (Lennox),Hopebio,HB0128 Agar,Sangon Biotech,A505255-0250 Sodium

(14) Swi/Snf Chromatin Remodeling Regulates Transcriptional Interference and Gene RepressionbioRxivApril 30, 2023Kaitlin Morse, Sarah Swerdlow, Elçin Ünalethanol and resuspended in 1X NEBuffer #2 (B7002S, New England Biolabs) with 2 μl RNase A (20 mg/ml, 12091021, Invitrogen). Samples were incubated for 30 min and run on a 2% agarose gel to examine digestion efficiency.

(15) Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesisNature CommunicationsApril 25, 2023Taiyu Chen, Marta Hojka, Philip Davey, Yaqi Sun, Gregory F. Dykes, Fei Zhou, Tracy Lawson, Peter J. Nixon, Yongjun Lin, Lu-Ning Liumin to pellet DNA. The resulting DNA was pre-eluted in 200 μL 1 M NaCl and 5 μL RNAse (Invitrogen, 12091021) at 65 °C for 5 h. Overall, 600 μL pre-cooled ethanol was added to the completely eluted sample, followed

(16) A maladaptive pleural environment suppresses preexisting anti-tumor activity of pleural infiltrating T cellsFrontiers in ImmunologyMarch 30, 2023Vera S. Donnenberg, James D. Luketich, Ibrahim Sultan, John Lister, David L. Bartlett, Sohini Ghosh, Albert D. Donnenbergstaining buffer, and resuspended in staining buffer containing PureLink RNAse A (Invitrogen, Cat. No. 12091-021, 1:400, 10 min 37°C) and washed. Finally, cells were resuspended in staining buffer to a concentration

(17) Unravelling the clinicopathological and functional significance of replication protein A (RPA) heterotrimeric complex in breast cancersnpj Breast CancerMarch 30, 2023Mashael Algethami, Michael S. Toss, Corinne L. Woodcock, Chandar Jaipal, Juliette Brownlie, Ahmed Shoqafi, Adel Alblihy, Katia A. Mesquita, Andrew R. Green, Nigel P. Mongan, et al.Ser139 (16202 A, Millipore, UK). For cell cycle analysis, cells were treated with 20 mg/ml RNase A (12091021, Invitrogen) and then 10 mg/ml Propidium Iodide (P4170, Sigma Aldrich) was added to determine the cell

(18) Interactions between FUS and the C-terminal Domain of Nup62 are Sufficient for their Co-phase Separation into Amorphous AssembliesJournal of molecular biologyMarch 15, 2023Kumar MS, Stallworth KM, Murthy AC, Lim SM, Li N, Jain A, Munro JB, Fawzi NL, Lagier-Tourenne C, Bosco DA.buffer for 1 h at ambient temperature. GST-FUS and GST were pretreated with 50 μg/ml RNase (Invitrogen, 12091021) for 30 min before mixing with MBP-Nup62 constructs to avoid any potential confounding effects of trace

(19) Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activationMolecular neurodegenerationMarch 7, 2023Todorova V, Stauffacher MF, Ravotto L, Nötzli S, Karademir D, Ebner LJA, Imsand C, Merolla L, Hauck SM, Samardzija M, et al.}$$\end{document}∘C for 45 min with shacking at 300 rpm and RNase A (100 mg/ml; Thermo Fisher Scientific, 12091021) was added to the samples. DNA isolation was performed with QIAamp DNA Blood Mini Kit (Qiagen, 51104)

(20) High-resolution mapping of mitotic DNA synthesis under conditions of replication stress in cultured cellsSTAR ProtocolsMarch 1, 2023Florian J. Groelly, Rebecca A. Dagg, Jonathan Mailler, Thanos D. Halazonetis, Madalena TarsounasPropidium iodide solution (1 mg/mL),Sigma-Aldrich,Cat# P4864 PureLink RNase A (20 mg/mL),Thermo Fisher,Cat# 12091021 Triton-X-100,Sigma-Aldrich,Cat# X100-500ML PBS,Thermo Fisher,Cat# BR0014G CuSO4,Sigma-Aldrich,Cat#

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PureLink RNase A (20 mg/mL) from Thermo Fisher Scientific

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PureLink RNase A (20 mg/mL) from Thermo Fisher Scientific

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