Fig 1: Characterization of αDC1s. (A) Typical schema for DC generation and αDC1 polarization. (B) αDC1s were analyzed for markers of antigen presentation, DC maturation/activation, and co-stimulation by flow cytometry. (C) IL-12p70 secretion was determined by ELISA after αDC1 incubation with recombinant CD40L. (D) αDC1s loaded with or without SIINFEKL peptide and co-cultured with activated OT1 CD8+ T cells for 48 hours. Cell-free supernatant was analyzed for IFN-gamma and IL-2 secretion by ELISA. *P < 0.05, bars ± STDEV.
Supplier Page from Sino Biological, Inc. for Mouse CD40 Ligand Protein (ECD, His Tag)