Fig 2: Correlation analysis between drug intervention and EMT.A UMAP plot of IL-17 and its receptor expression in EMT-associated cells. B UMAP plot of EMT score in EMT-associated cells. C UMAP plot showing the distribution of genes upregulated by ADA, SEC and UST in EMT-associated cells. Each dot denotes a single cell; the higher the cell score, the better the effect of the drug. D Correlation diagram of EMT and drug intervention.

Fig 3: Dynamic evolution process of EMT in psoriasis.A UMAP of 33118 FBs showing the EMT score. (B) Violin plot showing the EMT score of FBs, coloured by fibroblast subtypes. C UMAP plot of the 94,759 cells from 13 samples in the skin of mice, showing the EMT score. D Recluster and UMAP visualization of EMT-related subtype, including iFib1, iFib2, basal 3 hyperplasia KCs, EMT KCs, Mitotic_KCs and Spinous_KCs. Each dot denotes a single cell. E The EMT progress between fibroblasts and keratinocytes. F Temporal variation in an IL-17 signalling pathway marker (Il17ra), keratinocyte markers (Krt5), and EMT markers (Vim and Zeb2) in EMT-related subtypes. Each point indicates a single cell, coloured by cell subtype.

Fig 4: FB heterogeneity in psoriasis.A UMAP plot of 33,118 FBs coloured by cell subtype. Each dot denotes a single cell. B Heatmap showing differences in hallmark pathway activities scored per cell with GSVA among different fibroblast subtypes. (C) Dot plot of the expression levels of marker genes for FB subtypes defined in A. Dot size corresponds to the percent of expressing cells, and dot colour indicates the average expression levels. D UMAP plot of all fibroblast subtypes from the skin of the IMQ group and WT group, coloured by cell type. E Bulk RNA-seq and scRNA-seq analysis of the DEGs in the IMQ group and WT group (bulk RNA-seq data source GSM2299980 (WT), GSM2299981 (WT), GSM229998 (IMQ), GSM2299983 (IMQ)). F Violin plots showing the top DEGs between IMQ and WT in all fibroblast subtypes. G KEGG pathway map of IMQ vs. WT scRNA-seq in FBs. H Western blot image of Vim, Lcn2 and IL-17 expressions in HaCaT cells treated with IL-17 for indicated time. I–K Histogram of protein expression level in H. L–N Quantification of Vim, Lcn2 and IL-17 mRNA expressions in NHDF cells stimulated with IL-17 (20 ng/mL) for 6 h.