Fig 1: Dose-response curve depicting anti-SLIT2 mAb (AB7665) binding in the SLIT2/ROBO1 assay. Error bars indicate the standard deviation (n = 3).
Fig 2: (A) Scatterplot showing single-dose inhibition data screened at 100 μM of the MedChem Express protein-protein interaction inhibitor library using our SLIT2/ROBO1 TR-FRET assay. (B) Correlation plot of the screened chemical library duplicate runs in the SLIT2/ROBO1 TR-FRET assay.
Fig 3: Our established workflow for the SLIT2/ROBO1 TR-FRET assay.
Fig 4: Variation in the TR-FRET ratio in the SLIT2/ROBO1 as a function of the acceptor-to-donor ratio. Error bars indicate the standard deviation (n = 3).
Fig 5: Schematic representation of the TR-FRET assay designed for identifying small molecules that disrupt the SLIT2/ROBO1 interaction.
Supplier Page from Sino Biological, Inc. for Human ROBO1 Protein (ECD, Fc Tag)