Fig 1: Upregulation of PADI2, Citrullinated Peptides, and Citrullinated Vimentin in a silicosis model. A–F Immunofluorescence staining of Cit-peptide and vimentin (Vim) co-expression in silicotic rats and mice (scale bar = 50 μm). Western blot analysis showing the expression levels of PADI2, Cit-peptide, and Cit-Vim across groups (Results are presented as Mean ± SD, n = 6 per group). G–H Immunohistochemical staining of PADI2 and immunofluorescence staining of Cit-peptide and Vim co-expression in macrophage-depleted mice (scale bar = 50 μm). Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression (Results are presented as Mean ± SD, n = 3 per group)
Fig 2: PADI2 regulates citrullinated peptides and citrullinated vimentin expression. A Immunofluorescence staining of Cit-peptide and Vim co-expression (scale bar = 50 μm) and Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression in AFM-30a-treated mice. B Immunofluorescence staining of Cit-peptide and Vim co-expression (scale bar = 100 μm) and Western blot analysis of PADI2, Cit-peptide, and Cit-Vim expression in Padi2 knockout mice. Results are presented as Mean ± SD, n = 3 per group
Fig 3: Transcriptome Sequencing Reveals pathways and targets of Padi2 knock in Mitigating Pulmonary Fibrosis in Silicotic Mice. A Hierarchical cluster heatmaps showing gene expression in different groups. B–C GO and KEGG pathway analysis of downregulated and upregulated genes in the Padi2 knockout (KOS) group. D Heatmaps displaying significantly downregulated genes in the KOS Group. E–F Immunofluorescence staining of TNF-α expression (scale bar = 100 μm) in RAW 264.7 cells treated with Cit-Vim. G–H Western blot analysis of EGR1, TNF-α, IL-6 and IL-1β expression in RAW 264.7 cells treated with Cit-Vim. I–J Western blot analysis of TNF-α, IL-6 and IL-1β expression in Cit-Vim and siRNA-Egr1-treated RAW 264.7 cells. Results are presented as Mean ± SD, n = 3 per group
Supplier Page from Cayman Chemical for Citrullinated Vimentin (R144) (139-159)-biotin Peptide