Fig 1: (a) Representative bright-field images showing morphological changes in BV2 microglia following recombinant OSM stimulation. Compared with untreated cells, OSM-treated BV2 cells exhibited enlarged soma, shortened processes, and a more amoeboid-like morphology, consistent with microglial activation. (b) CCK8 assay detects the cell viability of each group; (c, d) The representative images and quantitative analysis of protein expression of IL-6, TNF-α, and β-actin detected by Western blot; Data were expressed as the mean±SEM, *P < 0.05, **P < 0.01, vs. indicated groups n = 3 for each group.