IL-4 Protein, Mouse (HEK293, His) HY-P70653 from MedChemExpress

Product Specs
ItemIL-4 Protein, Mouse (HEK293, His)
CompanyMedChemExpress
Price $250.00Supplier Page View Company Product Page
Catalog NumberHY-P70653
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
TypeProtein
Size50 µg
Conjugate/TagHis
Molecular Weight15-19 kDa
Source/Expression SystemHEK293
ActivityThe cell proliferation assay using M-NFS-60 mouse lymphoblast cells has an ED50 value of 0.035 ng/mL. Mouse IL-4-His on HIS1K Biosensor can bind Mouse IL-4RA-Fc with an affinity constant of 0.001 nM
SpeciesMouse
NCBI Full Gene Nameinterleukin 4
NCBI Gene AliasesIl-4, Il, BSF-1

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(1) Trem2 activation by renal tubular debris sustains Arg1+ macrophage survival and promotes tubular epithelial repair in renal ischemia-reperfusion injuryFront ImmunolApril 10, 2026Jiayu Wang, Zhiyi Zhao, Runyi Wang, Xin Zheng, Xiaopeng Humedium (Procell, CM-0190). RAW264.7 cells were treated with 20 ng/ml IL-4 protein (MedChemExpress, HY-P70653) for 24 hours to induce polarization. TCMK-1 cell debris were quantified under a microscope and added

(2) IMP2 enhances M2 macrophage polarization via LKB1-AMPK-mediated mitochondrial dynamics and fatty acid β-oxidation to ameliorate diabetic osteoporosisCellular and Molecular Life SciencesFebruary 27, 2026Lingshuang Li, Yajun Cui, Yu Ji, Ke Ma, Xuejie Lin, Ting Liu, Junyang Sun, Hongrui Liu, Jie Guo, Minqi Li5% CO₂. They were seeded at a density of 1 × 10⁵/mL in 6-well plates. After 24 h, 20 ng/mL IL-4 (MCE HY-P70653) and 20 ng/mL IL-13 (MCE HY-P70460) were added to induce for 48 h. The supernatant was collected as

(3) Maimendong Decoction Suppresses Non-Small Cell Lung Cancer Growth by Promoting Dendritic Cell Maturation via the SIRT1/p65 Acetylation PathwayiScienceFebruary 20, 2026Qinfeng Zhou, Yuchen Song, Yang Guo, Yuwen Hu, Zicheng Zhang, Keke Zhao, Lijun Ai, Kaixuan Wang, Cong Wang, Lei Wang, et al.L210KJ Recombinant mouse GM-CSF,MedChemExpress,Cat# HY-P7361 Recombinant mouse IL-4,MedChemExpress,Cat# HY-P70653 Cisplatin (DDP),Macklin,Cat# D807330 Proteinase K,Beyotime,Cat# ST532 Bovine serum albumin,Cell Signaling Technology

(4) Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma DedifferentiationAdvanced ScienceNovember 1, 2025Kang Ning, Bu Zou, Yongchao Yu, Taonong Cai, Zhenyu Luo, Yu Guo, Yi Wu, Xiujiao Shen, Hao Li, Mengyuan Fang, et al.macrophages. After a 24‐h resting period, cells were further stimulated with interleukin‐4 (IL‐4, MCE, HY‐P70653, 20 ng ml−1) and interleukin‐13 (IL‐13, MCE, HY‐P72595, 20 ng ml−1) for an additional 48 h to promote

(5) Macrophage-derived exosomes carrying miR-30a-5p alleviates IL-13/IL4-induced epithelial-mesenchymal transition in mouse bronchial epithelial cells via Runx1BMC Pulm MedOctober 28, 2025Bin Wu, Danhong Li, Yu Wang, Jieru Xu, Lihong Li, Mengjie Wangat 10 µM was used to inhibit EXO synthesis [27]. IL-13 (HY-P70460, MCE, USA) of 10 ng/mL and IL-4 (HY-P70653, MCE) of 10 ng/mL was used to induce EMT. Cells were co-cultured for 48 h. Isolation and identification

(6) Bioengineered hybrid dual-targeting nanoparticles reprogram the tumour microenvironment for deep glioblastoma photodynamic therapyNat CommunAugust 18, 2025Rongrong Zhao, Ying Hou, Boyan Li, Ziwen Pan, Jiawei Qiu, Qingtong Wang, Yanhua Qi, Zhe Han, Hongyu Zhao, Hong Liu, et al.R&D Systems (AF808, USA). Azide-choline (AEcho, HY-W588725), lipopolysaccharides (LPS, HY-D1056), IL4 (HY-P70653), cyanine 5.5 (HY-D0924), and cyanine5 DBCO (HY-D1583) were purchased from MCE (China). The Cell Counting Kit-8

(7) Microglial exosome TREM2 ameliorates ferroptosis and neuroinflammation in alzheimer's disease by activating the Wnt/β-catenin signalingSci RepJuly 10, 2025Ling Zhu, Tao Zhou, Lei Wu, Xuanang Zhu, Lvan Chen, Mi Zhang, Jing Zhou, Fan WangAll cells were grown in a 5% CO2, 37 °C incubator. The BV2 cells were treated with IL-4 (20 ng/mL, HY-P70653, MCE) for 48 h to induce their transformation into the M2 phenotype29. Following the previously described

(8) Piperlongumine Inhibits Lung Cancer Growth by Inducing Endoplasmic Reticulum Stress Leading to Suppression of M2 Macrophage PolarizationBiol Proced OnlineMay 22, 2025Yixin Zhou, Wenjin Teng, Jianchun Wu, Yingbin Luo, Yuli Wang, Yan LiUSA. It was dissolved in PBS to a 20 mM/L stock concentration. Interleukin-4 (IL-4)( catalog number: HY-P70653) was purchased from MedChemExpress, USA. The lyophilized powder was reconstituted in sterile deionized

(9) A bifunctional fusion membrane-based biocompatible nanovaccine to potentiate cancer immunotherapyTheranosticsApril 21, 2025Wei Fu, Xing Cai, Jinru Yang, Lian Yang, Yaoyu Pan, Zhan TuoSigma-Aldrich (96992). GM-CSF was purchased from MCE (HY-P7361). Interleukin-4 (IL-4) was purchased from MCE (HY-P70653). Polystyrene and polyethylene were purchased from Sigma-Aldrich (450383, 428043). FeCl3 and dimethoxymethane

(10) A double-edged sword role of IFN-γ-producing iNKT cells in sepsis: Persistent suppression of Treg cell formation in an Nr4a1-dependent manneriScienceDecember 20, 2024Yingyu Qin, Yilin Qian, Shengqiu Liu, Rong ChenRecombinant Protein,UA BiOSCIENCE,Cat# UA040172 Mouse IL-4 Recombinant Protein,MedChemExpress,Cat# HY-P70653 α-Galactosylceramide (KRN7000),Avanti Research,Cat# 867000 ,, Critical commercial assays,Critical

(11) Programmable Bacteria with Dynamic Virulence Modulation System for Precision Antitumor ImmunityAdvanced ScienceJuly 26, 2024Leyang Wu, Lin Li, Liyuan Qiao, Chenyang Li, Shuhui Zhang, Xingpeng Yin, Zengzheng Du, Ying Sun, Jiahui Qiu, Xiaoyao Chang, et al.Assays The macrophage line RAW264.7 was induced to the M2‐type using IL4 protein (MedChemExpress, HY‐P70653, Shanghai, China). Total intracellular RNA was extracted using a total RNA isolation kit (Vazyme, RC112),

(12) Novel prognostic biomarker TBC1D1 is associated with immunotherapy resistance in gliomasFront ImmunolMarch 11, 2024Daqiang Song, Qian Yang, Liuying Li, Yuxian Wei, Chong Zhang, Huimin Du, Guosheng Ren, Hongzhong Lito obtain mature M0 macrophages by day 7, while M2 macrophages were induced by IL4 protein (1 ng/mL, HY-P70653, MedChemExpress), M1 macrophages by lipopolysaccharide stimulation, and MDSC differentiation was initiated

(13) Regulation of IL-4 Expression in Immunity and DiseasesAdvances in experimental medicine and biologyJanuary 1, 2016Ho IC, Miaw SC.

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IL-4 Protein, Mouse (HEK293, His) from MedChemExpress

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IL-4 Protein, Mouse (HEK293, His) from MedChemExpress

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