Fig 1: Validation of PLA2G7-positive macrophages driving inflammatory activation in IPF through single-cell sequencing analysis. (A) Heatmap depicting marker gene expression for cell annotation in different cell types. (B) t-SNE dimensionality reduction landscape of single-cell sequencing data. (C) Differential expression of PLA2G7 in IPF and healthy samples. (D) Differential analysis of different cell types in IPF and healthy samples. (E) t-SNE dimensionality reduction landscape of single-cell sequencing data samples from mice. (F) t-SNE dimensionality reduction landscape of cell types in the single-cell sequencing data from mice. (G) Heatmap displaying the marker gene expression in different cell types. (H) t-SNE dimensionality reduction landscape after re-extraction of macrophages in the single-cell sequencing data from mice. (I) Bubble plot exhibiting the expression levels of PLA2G7 and SPP1 in different samples. (J) t-SNE plot displaying the expression levels of PLA2G7 in different mouse samples. (K) In human single-cell sequencing data, violin plot depicting PLA2G7 expression in PLA2G7-positive and PLA2G7-negative macrophages. (L) In human single-cell sequencing data, differential analysis of PLA2G7-positive and PLA2G7-negative macrophages. (M) In human single-cell sequencing data, GSEA of PLA2G7-positive and PLA2G7-negative macrophages. (N) H&E, Masson’s trichrome staining and Multiplex immunofluorescence in a mouse model. (O) Counting of Cd68 + Pla2g7 + cells in a mouse model. (P) ELISA for Pla2g7 expression levels in alveolar lavage fluid and lung tissues of the mouse model.