Fig 1: MiR-143-3p inhibited the osteogenic differentiation of BMSCs through targeting Igfbp5. (A) Prediction of miR-143-5p target genes by databases. (B) Gene sequences for the dual-luciferase reporter assay. (C) Fluorescence intensity of the dual-luciferase reporter assay. (D) IGFBP5 expression levels in BMSCs. Effects of mimic and inhibitor on IGFBP5 mRNA (E) and protein (F) levels. (G) Immunofluorescence images of IGFBP5 in periodontal tissues of mice with periodontitis. Scale bar: 200 μm. (H) Relative fluorescence intensity of IGFBP5 in bone tissue. Effects of IGFBP5 and its inhibitor on mRNA levels of BMSC mineralization markers (I), ALP activity (J), ARS and ALP staining (K), and protein levels of mineralization markers (L). n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001