Fig 1: The NET-CCDC25 axis downregulates activating receptors of NK cell.a RT-qPCR screening assay assessing the transcription of activating receptor, cytotoxic granule protein and anti-tumor cytokine genes (Left), and inhibitory receptor genes (Right) in human NK cells treated with or without NETs (n = 4 biologically independent experiments). b Flow cytometry analysis showing the expression of NKG2D, NKp46, NKp44, 2B4 and DNAM-1 on human NK cells (CD3- CD56+) treated as indicated in vitro (n = 3 biologically independent experiments). MFI, median fluorescence intensity. c Flow cytometry analysis showing the expression of NKG2D, NKp46, 2B4 and DNAM-1 in murine NK cells, untreated or pre-treated with αCCDC25 antibody or IgG Isotype control, followed by NET stimulation (n = 3 biologically independent experiments). MFI, median fluorescence intensity. d–g Flow cytometry analysis showing the frequency of CD107a+ human NK cells (d, e) or murine NK cells (f, g) pre-treated with either IgG or αCCDC25 antibody, prior to stimulation with NETs or DNase I-digested NETs (n = 3 biologically independent experiments). h, i, Flow cytometry analysis showing the frequency of CD107a+ NK cells (CD3- NK1.1+), the expression of NKG2D (h), and the frequency (i) of the liver-infiltrating NK cells from C57BL/6J mice on day 30 post-inoculation with EO771 cells, with αCCDC25 antibody or Isotype-IgG administration (n = 5 mice per group). MFI, median fluorescence intensity. Data are presented as mean ± SD (a–c, e, g, h, i) and statistical analysis was performed using Welch’s unpaired two-tailed t test (a, NKG2D in h), unpaired two-tailed t test (CD107a in h) or one-way ANOVA with Tukey’s test (b, c, e, g). Source data are provided as a Source Data file.