Fig 1: Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples from PDGF-BB (HY-P7087, MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p-values are annotated for indicated comparisons.