Fig 2: Effects of miR-34a and FGF21 in ApoE−/− mice and HAoECs. (A) Venn plots for GeneCards database search for senescence and AS-related miRNA. (B) Top 10 miRNAs with the highest correlation scores associated with senescence in the common 276 miRNAs. (C) QPCR was used to detect the expression level of miR-34a in ApoE−/− mice at 12 weeks and HAoECs at 24 h. n = 3. (D) QPCR analysis of FGF21 mRNA expression in ApoE−/− mice at 12 weeks and HAoECs at 24 h. n = 3. (E) QPCR was used to detect the expression levels of miR-34a in HAoECs after treatment with the miR-34a mimic or inhibitor at 24 h. n = 3. (F) QPCR analysis of FGF21 mRNA expression in HAoECs after treatment with the miR-34a mimic or inhibitor at 24 h. n = 3. (G) The CCK-8 method was used to evaluate the effect of the miR-34a mimic on the proliferation of HAoECs at 96 h. n = 3. (H) CCK-8 was used to detect the effect of the miR-34a inhibitor on the proliferation of HAoECs at 96 h. n = 3. (I) A representative image of SA-β-Gal-stained HAoECs after treatment with the miR-34a mimic or inhibitor at 24 h. n = 5. Bar = 100 μm. (J) Statistical analysis of the stained area in (I). n = 5. (K) A representative image of γ-H2AX staining of HAoECs after treatment with the miR-34a mimic or inhibitor at 24 h. Bar = 20 μm. (L) Statistical analysis of the number of DNA damage foci in (K). n = 5. (M) QPCR analysis of the mRNA expression of the inflammatory factors IL-1β, IL-6, IL-8 and TNF-α in HAoECs after miR-34a mimic treatment at 24 h. n = 3. (N) QPCR analysis of the mRNA expression of the inflammatory factors IL-1β, IL-6, IL-8 and TNF-α in HAoECs after miR-34a inhibitor treatment at 24 h. n = 3. (O) QPCR was used to detect the relative expression levels of the cell cycle-related genes p53, p21, p16 and Rb in HAoECs after miR-34a mimic treatment at 24 h. n = 3. (P) QPCR was used to detect the relative expression levels of the cell cycle-related genes p53, p21, p16 and Rb in HAoECs in different treatment groups after miR-34a-5p inhibitor treatment at 24 h. n = 3. (Q) Western blotting was used to detect the protein expression of p53, p21, and Rb in HAoECs after miR-34a mimic or inhibitor treatment at 24 h. n = 3. (R) Quantitative analysis of the results in (Q). *p < 0.05, **p < 0.01, ***p < 0.001, vs. the NFD group or the control group or mimic-NC; ##p < 0.01, ###p < 0.001, vs. HFD group or H2O2 group or inhibitor-NC.