Fig 1: Amifostine inhibits the upregulation of CD44 and blocks its binding with MIF in macrophages.N = 6 in Panel E, ***p < 0.001.(A) Representative images of immunofluorescence staining of 8-oxoG.(B) Representative images of NRF2 immunofluorescence staining in macrophages.(C) Representative images of immunofluorescence staining of F4/80, CD44, and MIF in SI slides.(D) Representative images of organoids.(E) Bar graph showing the average area of organoids.
Fig 2: Activation of CD44 in macrophages is a vital factor in 15 Gy ABR-induced mouse death.N = 6 in Panels B–D; n = 12 in Panels G–I, ***p < 0.001.(A) Representative FACS images showing the gating for SI macrophages and the expression of CD74, CD44, and CXCR4.(B) Bar graph showing the mean fluorescence intensity (MFI) of CD74; (C) CD44; and (D) CXCR4.(E) Representative images of immunofluorescence staining of F4/80, CD44, and MIF in SI slides.(F) Experimental scheme of isotype/CD44 antibody treatment.(G) The survival rate of mice administered isotype/CD44 antibody immediately, (H) on the second day, and (I) on the fourth day after 15 Gy ABR exposure.
Fig 3: CellChat analysis of communications among all the clusters.(A) Heatmap showing the predicted interaction signaling pattern among all the clusters.(B) Dot plots showing the differentially enriched GO terms in macrophages.(C) Heatmap showing regulon analysis to evaluate the differential expression of transcription factors in macrophages.(D) Representative images of NRF2 immunofluorescence staining in macrophages.(E) The underlying signaling network of MIF with CD74−CD44/CXCR2/CXCR4.(F) Violin plots show the expression of MIF and its receptors.(G) Ligand‒receptor interaction between macrophages and neutrophils.(H) Violin plots showing gene expression in macrophages and neutrophils.
Supplier Page from MedChemExpress for MIF Protein, Mouse