Fig 2: MiR-145a-5p can partially reverse the chondrogenic inhibition caused by IL-1 b.(A) MiR-145a-5p in ATDC5 cells was detected via RNA FISH after treatment with IL-1 β. (B) Fluorescence intensity was calculated using ImageJ. (C) The expression levels of miR-145a-5p, Sox9, Acan, and Col2 in the Ctrl and IL-1 β groups were detected via qRT‒PCR. (D) The expression levels of Sox9, Col2 and Acan in the miR-NC and miR-mimic groups with or without IL-1 β were detected. (E) Alcian blue staining was performed in the four groups. (*p < 0.05, **p < 0.01, ***p < 0.005 and ****p < 0.001).