Fig 1: mtiRL enhances the binding between Yes1 and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that active GST‐Yes1 could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.