Fig 1: VEGFA Reverses the Effects of F3 Knockdown on CD31-Positive Vascular Changes. (A) Representative images of Safranin O/Fast Green staining of the tibial plateau in the Model, F3 siRNA, and F3 siRNA + VEGFA groups (overall view: magnification, 5×; scale bar = 500 µm; magnified view: magnification, 50×; scale bar = 50 µm). (B,C) Representative immunofluorescence images (B) and quantitative analysis of fluorescence intensity (C) for F3 protein in the tibial plateau across groups (magnification, 5×; scale bar = 200 µm). (D–F) Representative images (D) and double-immunofluorescence staining in the tibial plateau (magnification, 5×; scale bar = 200 µm), (E) quantitative evaluation of fluorescence intensity for CD31 (F) and COL2A1. not significant (ns) = p > 0.05, * p < 0.05, *** p < 0.001.